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首页> 外文期刊>Bulletin of the National Research Centre >Transposon (Tn5) and UV Irradiation as Mutagenic Agents for Improving Photorhabdus sp. Sinai 1 (S1), an Insect-Pathogenic Bacterium
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Transposon (Tn5) and UV Irradiation as Mutagenic Agents for Improving Photorhabdus sp. Sinai 1 (S1), an Insect-Pathogenic Bacterium

机译：转座子（Tn5）和紫外线照射作为致突变剂，以改善光生藻。 Sinai 1（S1），一种昆虫致病性细菌

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Alocal isolate of symbiotic bacterium, Photorhabdus sp. Sinai 1 (S1) was isolated from Heterorliabditis sp. S1 insect-pathogenic nematodes and its character was detected. Antibacterial/antifungal antibiotic(s) and proteinase productions of Photorhabdus S1 play an important role in killing insects. Therefore, Photorhabdus sp. (S1) was treated with UV for different times. Survival percentage of the original strain dropped from 100% at zero time to 11.13%, 0.23 and 0.022% after UV treatment for 1, 3 and 5 min, respectively. However, 32 mutants were isolated, 18 after one min treatment and 14 after 3min treatmertt. On the other hand, wide ranges of proteinase production capacities were detected. Eight of the mutants isolated after UV treatment for 1 min, had lower production than SI, while 4 mutants had the same productivity as S1 Six mutants of 1 min UV treatment, were higher producers than S1. Moreover, 14 mutants were isolated after UV treatment for three min. Three of them had the same proteinase production as S1, 3 had lower production, and about 8 proved to be highly producer mutants. Mutant No. 20 from UV 3 min treatment group proved to be the best producer. It produced 25% over than S1. Suicidal plasmid pSUP2021 was mobilized from Escherichia coli to Photorhabdus by transconjugation and 15 transconjugants were isolated. All of them proved electrophoritically to have the plasmid. Fourteen transconjugants were tested for their proteinase productivity. However, only two transconjugants (14%) were higher proteinase producers, one of them reached 133.3 % in comparison with S1. Moreover, all transconjugants except one were higher antibacterial antibiotic producers than S1. Transconjugant No. 7 proved to be the best producer one. It produced about 150% antibacterial and 160% antifungal antibiotics in comparison with S1 parental strain. Results also indicated that, Photorliabdus Simulations due to transposon Tn5 mutagenesis were more powerful than those induced by UV in proteinase production. Transposon Tn5 mutagenesis is one of the most important tool for antibiotic improvement in Photorhabdus.
机译：共生细菌Photorhabdus sp。的局部分离株。 Sinai 1（S1）分离自Heterorliabditis sp.。 S1昆虫致病线虫和其特征被检测到。 Photorhabdus S1的抗菌/抗真菌抗生素和蛋白酶生产在杀死昆虫方面起着重要作用。因此，Photorhabdus sp。 （S1）用紫外线处理了不同的时间。紫外线处理1分钟，3分钟和5分钟后，原始菌株的存活率从零时的100％分别降至11.13％，0.23和0.022％。然而，分离到32个突变体，在处理1分钟后分离出18个，在处理3分钟后分离出14个。另一方面，检测到广泛的蛋白酶生产能力。 UV处理1分钟后分离出的8个突变体的产量低于SI，而4个突变体具有与S1相同的生产力。UV处理1 min的六个突变体的产量高于S1。此外，在UV处理3分钟后分离出14个突变体。其中三个具有与S1相同的蛋白酶生产，三个具有较低的蛋白酶生产，并且大约8个被证明是高产量突变体。紫外线3分钟处理组的20号突变体被证明是最好的生产者。它的产量比S1高25％。通过转缀合将自杀质粒pSUP2021从大肠杆菌动员到Photorhabdus，并分离出15个转缀合剂。所有这些均通过电泳证明具有质粒。测试了十四个转导结合剂的蛋白酶生产力。但是，只有两个转导结合体（14％）是较高的蛋白酶生产者，其中一个与S1相比达到133.3％。此外，除一个外，所有转导结合剂的抗菌素产生率均高于S1。事实证明，第7号转导结合体是最好的生产者。与S1亲本菌株相比，它产生约150％的抗菌和160％的抗真菌抗生素。结果还表明，转座子Tn5诱变引起的Photorliabdus模拟比蛋白酶诱导的UV诱导更强大。转座子Tn5诱变是Photorhabdus中改善抗生素的最重要工具之一。

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