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首页> 外文期刊>Bulletin of the National Research Centre >Chemical Composition, Antifungal and Radical Scavenging Activities of Egyptian Mandarin Petitgrain Essential Oil
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Chemical Composition, Antifungal and Radical Scavenging Activities of Egyptian Mandarin Petitgrain Essential Oil

机译：埃及普通小花香精油的化学成分，抗真菌和清除自由基的作用

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摘要

The composition and biological activities of Egyptian mandarin petitgrain essential oil and its fractions are reported. The gas chromatographic-mass spectroscopic (GC/MS) study of this oil and its oxygenated and non-oxygenated fractions revealed that the major components were methyl-n-methyl anthranilate (62.82%), m-terpincne (17.2%), p-cymene (3.52%) and α-pinene (2.67%) for total oil; methyl-n-methyl anthranilate (85.89%), caryophyllene oxide (1.85%) and hexdecaoic acid (1.37%) for oxygenated fraction compared to γ-terpinene (52.65%). caryophellene (10.3%), α-pinene (6.1%), p-cymene (5.91%) and gurunene (4.47%) for non-oxygenated fraction. The antifungal efficacy of the mandarin oil and its fractions when examined by disc diffuxion test at different comcentrations showed that the mandarine oil and its oxygenated fraction (at 75 μL) strongly inhibited the growth of all the tested fungi (75-99%). This activity might be due to the presence of high content of methyl-n-methyl anthranilate. In contrast, the non-oxygenated fraction was less effective against the tested fungi (5-26% inhibition) under the same conditions. The mandarin petitgrain oil and its fractions were further investigated for radical scavenging activities using 1,1-diphenylpicryl-2-picrylhydrazyl (DPPH). At a concentration of 2500 ppm, all the tested samples showed radical scavenging activity. Obviously, the oxygenated fraction (mainly methyl-n-methyl anthranilate) exhibited higher significant reduction of DPPH radicals (86%) as compared with BHA (85%) and gallic acid (76%). The present results support the view that the Egyptian mandarin petitgrain essential oil is highly bioavailable and may be considered as safe flavouring agent with pleasent aroma in addition to being antioxidant with free radical scavengers. Also its biological activity is shown to be particularly interseting for food and cosmetics industries.
机译：据报道埃及普通话小花香精油及其馏分的组成和生物活性。对该油及其含氧化合物和非含氧化合物的气相色谱-质谱（GC / MS）研究表明，主要成分为邻氨基苯甲酸甲酯-N-甲基（62.82％），间-叔戊烯（17.2％），对-甲苯磺酸。总油中异丙苯（3.52％）和α-pine烯（2.67％）;与γ-萜品烯（52.65％）相比，邻苯二甲酸甲酯（85.89％），石竹烯氧化物（1.85％）和十六碳酸（1.37％）的氧化部分更高。非氧化级分的邻苯二酚（10.3％），α-pine烯（6.1％），对伞花烯（5.91％）和古伦烯（4.47％）。通过圆盘扩散试验在不同浓度下对普通话油及其馏分的抗真菌功效表明，普通话油及其含氧馏分（75μL）强烈抑制所有被测真菌的生长（75-99％）。该活性可能是由于存在高含量邻氨基苯甲酸甲酯。相反，在相同条件下，未氧化的部分对测试真菌的效果较差（抑制率为5-26％）。进一步研究了普通柑桔油及其馏分的自由基清除活性，使用1,1-二苯基吡啶-2-吡啶并肼基（DPPH）。在2500 ppm的浓度下，所有测试样品均表现出自由基清除活性。显然，与BHA（85％）和没食子酸（76％）相比，含氧馏分（主要是邻氨基苯甲酸甲酯）显着降低了DPPH自由基（86％）。目前的结果支持了这样的观点，即埃及普通话petitgrain精油具有很高的生物利用度，除具有自由基清除剂的抗氧化剂作用外，还可被视为具有令人愉悦香气的安全调味剂。同样，它的生物活性对于食品和化妆品行业尤为重要。

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