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首页> 外文期刊>Cell Research >A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure
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A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

机译：经过体外受精程序，由单核卵母细胞衍生的高度纯合和孤雌性人类胚胎干细胞系

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Homozygous human embryonic stem cells (hESCs) are thought to be better cell sources for hESC banking because their human leukocyte antigen (HLA) haplotype would strongly increase the degree of matching for certain populations with relatively smaller cohorts of cell lines. Homozygous hESCs can be generated from parthenogenetic embryos, but only heterozygous hESCs have been established using the current strategy to artificially activate the oocyte without second polar body extrusion. Here we report the first successful derivation of a human homozygous ESC line (chHES-32) from a one-pronuclear oocyte following routine in vitro fertilization treatment. chHES-32 cells express common markers and genes with normal hESCs. They have been propagated in an undifferentiated state for more than a year ( > P50) and have maintained a stable karyotype of 46, XX. When differentiated in vivo and in vitro, chHES-32 cells can form derivatives from all three embryonic germ layers. The almost undetectable expression of five paternally expressed imprinted genes and their HLA genotype identical to the oocyte donor indicated their parthenogenetic origin. Using genome-wide single-nucleotide polymorphism analysis and DNA fingerprinting, the homozygosity of chHES-32 cells was further confirmed. The results indicated that 'unwanted' one-prbnuclear oocytes might be a potential source for human homozygous and parthenogenetic ESCs, and suggested an alternative strategy for obtaining homozygous hESC lines from parthenogenetic haploid oocytes.
机译：纯合的人类胚胎干细胞（hESC）被认为是hESC储存的更好的细胞来源，因为它们的人类白细胞抗原（HLA）单倍型将大大增加某些群体相对较小的细胞系的匹配程度。可以从孤雌生殖的胚胎中产生纯合的hESC，但是使用当前的策略仅在人工激活卵母细胞而无需第二极体挤出的情况下，才建立了杂合的hESC。在这里，我们报告常规体外受精治疗后，从一个原核卵母细胞中首次成功获得人纯合ESC系（chHES-32）。 chHES-32细胞表达具有正常hESC的共同标记和基因。它们已经以未分化状态繁殖了一年以上（> P50），并保持了46，XX的稳定核型。当在体内和体外分化时，chHES-32细胞可以从所有三个胚芽层形成衍生物。五个父本表达的印迹基因的几乎检测不到的表达及其与卵母细胞供体相同的HLA基因型表明其孤雌生殖起源。使用全基因组单核苷酸多态性分析和DNA指纹图谱，进一步证实了chHES-32细胞的纯合性。结果表明“不需要”的单核卵母细胞可能是人纯合和单性生殖ESC的潜在来源，并提出了从单性单倍体卵母细胞获得纯合hESC系的替代策略。

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