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首页> 外文期刊>Cell Research >Spontaneous Ca2+ oscillations in subcellular compartments of vascular smooth muscle cells rely on different Ca2+ pools.
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Spontaneous Ca2+ oscillations in subcellular compartments of vascular smooth muscle cells rely on different Ca2+ pools.

机译：血管平滑肌细胞亚细胞区的自发Ca2 +振荡依赖于不同的Ca2 +库。

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摘要

Spontaneous Ca2+ oscillations in vascular smooth muscle cells have been modeled using a single Ca2+ pool. This report describes spontaneous Ca2+ oscillations dependent on two separate Ca2+ sources for the nuclear versus cytoplasmic compartments. Changes in free intracellular Ca2+ were monitored with ratiometric Ca2+- fluorophores using confocal microscopy. On average, spontaneous oscillations developed in 79% of rat aortic smooth muscle cells that were synchronous between the cytoplasm and nucleus. Reduction of extracellular Ca2+ (less than 1uM)decreased the frequency and amplitude of the cytoplasmic oscillations with 48% of the oscillations asynchronous between the nuclear and cytoplasmic compartments. Similar results were obtained with the Ca2+ channel blockers, nimodipine and diltiazem. Arg-vasopressin (AVP) induced a rapid release of intracellular Ca2+ stores that was greater in the nuclear compartment (4.20 +/- 0.23 ratio units, n = 56) than cytoplasm (2.54 +/- 0.28) in cells that had spontaneously developed prior oscillations. Conversely, cells in the same conditions lacking oscillations had a greater AVP-induced Ca2+ transient in the cytoplasm (4.99 +/- 0.66, n = 17) than in the nucleus (2.67 +/- 0.29). Pre-treatment with Ca2+ channel blockers depressed the AVP responses in both compartments with the cytoplasmic Ca2+ most diminished. Depletion of internal Ca2+ stores prior to AVP exposure blunted the nuclear response, mimicking the response of cells that lacked prior oscillations. Spontaneous oscillating cells had a greater sarcoplasmic reticulum network than cells that did not oscillate. We propose that spontaneous nuclear oscillations rely on perinuclear sarcoplasmic reticulum stores, while the cytoplasmic oscillations rely on Ca2+ influx.
机译：已使用单个Ca2 +库模拟了血管平滑肌细胞中的自发Ca2 +振荡。该报告描述了自发的Ca2 +振荡，取决于两个独立的Ca2 +来源，分别用于细胞核和细胞质区室。使用共聚焦显微镜通过比例式Ca2 +-荧光团监测游离细胞内Ca2 +的变化。平均而言，在79％的大鼠主动脉平滑肌细胞中会发生自发振荡，这些细胞在细胞质和细胞核之间同步发生。胞外Ca2 +的减少（小于1uM）降低了细胞质振荡的频率和幅度，其中48％的振荡在核和细胞质区室之间是异步的。使用Ca2 +通道阻滞剂，尼莫地平和地尔硫卓获得了相似的结果。精氨酸加压素（AVP）诱导细胞内Ca2 +储存的快速释放，其在核室（4.20 +/- 0.23比率单位，n = 56）中比在先前自发形成的细胞中的细胞质（2.54 +/- 0.28）更大。振荡。相反，在相同条件下没有振荡的细胞在细胞质中（4.99 +/- 0.66，n = 17）比在细胞核中（2.67 +/- 0.29）具有更大的AVP诱导的Ca2 +瞬变。用Ca2 +通道阻滞剂进行预处理可降低两个区室中的AVP反应，其中胞质Ca2 +减少最多。在AVP暴露之前内部Ca2 +储存的枯竭削弱了核反应，模仿了缺乏先前振荡的细胞的反应。自发振荡细胞比不振荡细胞具有更大的肌浆网。我们建议自发核振荡依赖于核周肌质网存储，而细胞质振荡依赖于Ca2 +涌入。

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