The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation.

Yin ZM; Sima J; Wu YF; Zhu J; Jiang Y

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The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation.

机译：JNK2 C端片段对p53稳定性和细胞增殖的影响。

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The basal activity of JNK is low in normal growing cells and inactivated JNK targets p53 for ubiquitination. To elucidate if the C-terminal part of JNK is responsible for its binding to p53, the low background tet-off inducible NIH3T3 cell line was selected by luciferase reporter gene and a double stable C-JNK Aa (203-424) cell line was established. After withdrawing tetracycline, the C-JNK fragment expression was induced and cell growth was dramatically inhibited 24 h later. However, the expresion of p53 was found to be increased after the induction of C-JNK fragment, evaluated by transfecting p21waf-luciferase reporter genes. Our further studies showed that C-JNK fragment could form complex with p53 both in vivo and in vitro. Induction of C-JNK fragment in vivo can increase p53 stability by inhibiting p53 ubiquitination.

机译：在正常生长的细胞中，JNK的基础活性低，失活的JNK靶向p53进行泛素化。为了阐明JNK的C末端部分是否负责其与p53的结合，通过荧光素酶报道基因选择了低背景tet-off诱导型NIH3T3细胞系，并使用了双重稳定的C-JNK Aa（203-424）细胞系。成立。退出四环素后，诱导C-JNK片段表达，并在24小时后显着抑制细胞生长。然而，通过转染p21waf-萤光素荧光素酶报告基因评估了诱导C-JNK片段后p53的表达增加。我们的进一步研究表明，C-JNK片段在体内和体外均可与p53形成复合物。体内诱导C-JNK片段可通过抑制p53泛素化来增加p53稳定性。

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来源
《Cell Research》 |2004年第5期|P.434-438|共5页
作者
Yin ZM; Sima J; Wu YF; Zhu J; Jiang Y;
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作者单位

Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology, College of Life Science, Nanjing Normal University, Nanjing 210097, China.;

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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Carbon ion; Assault by stabbing; in vivo; cell proliferation; Genes; Reporter; 基因; 报告;

机译：Carbon ion;Assault by stabbing;in vivo;cell proliferation;Genes;Reporter;基因;报告;

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专利

1. The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation. [J] . Yin ZM, Sima J, Wu YF, Cell research. . 2004,第5期

机译：JNK2 C末端片段对p53稳定性和细胞增殖的影响。
2. The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation [J] . 细胞研究（英文版） . 2004,第005期

机译：JNK2 C末端片段对p53稳定性和细胞增殖的影响
3. JNK2: a negative regulator of cellular proliferation. [J] . Sabapathy K, Wagner EF Cell cycle . 2004,第12期

机译：JNK2：细胞增殖的负调节剂。
4. Investigation of Aβ42 C-Terminal Fragments as Inhibitors of Aβ42 Assembly and Neurotoxicity [C] . Huiyuan Li, Bernhard H. Monien, Aleksey Lomakin American Peptide Symposium . 2009

机译：Aβ42C末端片段的研究作为Aβ42组装和神经毒性的抑制剂
5. Stathmin Depletion from Cells Lacking p53 Leads to Delayed Cell Cycle Progression and Apoptosis Due to Increased Microtubule Stability. [D] . Carney, Bruce K. 2011

机译：缺乏p53的细胞中的stathmin耗竭会导致细胞周期进程的延迟和细胞凋亡，这归因于微管稳定性的提高。
6. Induced N- and C-terminal cleavage of p53: a core fragment of p53 generated by interaction with damaged DNA promotes cleavage of the N-terminus of full-length p53 whereas ssDNA induces C-terminal cleavage of p53. [O] . A L Okorokov, F Ponchel, J Milner 1997

机译：诱导的p53的N和C末端切割：与受损DNA相互作用产生的p53核心片段促进了全长p53的N末端的切割而ssDNA诱导p53的C末端切割。
7. The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation [O] . Zhi Min YIN, Jian SIMA, Yi Fan WU, 2004

机译：JNK2 C-末端片段对P53和细胞增殖稳定性的影响
8. Endothelial Cell Apoptosis Induced by Fibrinogen Gamma Chain C-Terminal Fragment. [R] . Takada, Y., Akakura, N. 2005

机译：纤维蛋白原γ链C末端片段诱导内皮细胞凋亡。

The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation.

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