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首页> 外文期刊>Cell Research >Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells
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Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells

机译：Moschatin（一种从南瓜成熟种子（Cucurbita moschata）成熟的I型核糖体失活的新型蛋白）的纯化和表征，及其针对人黑素瘤细胞的免疫毒素的制备

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摘要

A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of approx 29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC_(50) of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M_(21) with a IC_(50) of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.
机译：已经使用硫酸铵沉淀，CM-纤维素52柱色谱，Blue Sepharose CL-6B Affinity柱色谱和FPLC大小排阻法成功地将南瓜成熟种子（Cucurbita moschata）的一种新型核糖体失活蛋白Moschatin纯化至均质。柱层析。 Moschatin是1型RIP，pI为9.4，分子量约为29 kD。它是一种rRNA N-糖苷酶，可有效阻断兔网织红细胞裂解物中的蛋白质合成，IC_（50）为0.26 nM。用抗人黑素瘤McAb Ng76成功制备了新型免疫毒素Moschatin-Ng76，它能有效抑制靶向黑素瘤细胞M_（21）的生长，IC_（50）为0.04 nM，比游离Moschatin低1500倍。 。结果表明，Moschatin可作为一种潜在的新型抗癌药。

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