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首页> 外文期刊>Cell Research >Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization
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Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization

机译：抑制性消减杂交技术鉴定人子宫平滑肌瘤上调基因

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摘要

In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88 genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes were firstly associated with UL. Three genes with notable difference were selected for Northern confirmation. Our results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA_2) showed up-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obvious expression in prostate, testis, liver, heart and skeletal muscle.
机译：在人子宫平滑肌瘤（UL）中寻找差异表达的基因时，采用抑制消减杂交技术构建了一个UL上调文库，该文库代表了88个基因。经过反向Northern分析的两轮筛选后，证实有20个基因被上调，包括17个已知基因和3个功能未知的基因。所有这些基因首先与UL相关。选择具有显着差异的三个基因进行Northern确认。我们的结果证明了这二十个基因的真实性。一种名为磷脂酶A2（PLA_2）的基因在4/6的患者中表达上调，并且对组织分布的研究表明它在前列腺，睾丸，肝脏，心脏和骨骼肌中有明显的表达。

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