Using a non-radioisotopic, quantitative TRAP-based me- thod detecting telomerase activities in human hepatoma cells

ZHANG RU GANG; XING WANG WANG; JIN HUI YUAN

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Using a non-radioisotopic, quantitative TRAP-based me- thod detecting telomerase activities in human hepatoma cells

机译：使用非放射性同位素，基于定量TRAP的方法检测人肝癌细胞中的端粒酶活性

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A non-radioisotopic, quantitative TRAP-based telom- erase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with Conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found Telomerase activities were absent in normal human liver Cells, while detected in all of four human hepatoma cell Lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.

机译：主要通过使用SYBR Green-I染色代替放射性同位素建立了非放射性同位素，基于定量TRAP的端粒擦除活性测定法。与传统的基于放射性同位素的方法相比，其可重复性和准确性更高。使用这种方法，我们发现正常人肝细胞中不存在端粒酶活性，而在所有四种人肝癌细胞系（BEL-7404，SMMC-7721，QGY-7903和HCCM）中均检测到端粒酶活性，而无显着差异。

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来源
《Cell Research》 |2000年第1期|p.71-77|共7页
作者
ZHANG RU GANG; XING WANG WANG; JIN HUI YUAN;
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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Telmoerase; non-radioisotopic telmoerase assay; human liver cells;

机译：端粒酶非放射性同位素端粒酶测定;人肝细胞;

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1. Using a non-radioisotopic, quantitative TRAP-based mernthod detecting telomerase activities in human hepatoma rncells [J] . 细胞研究（英文版） . 2000,第001期

机译：使用非放射性同位素，定量基于TRAP的方法检测人肝癌细胞rncell中的端粒酶活性
2. Acyclic retinoid induces partial differentiation, down-regulates telomerase reverse transcriptase mRNA expression and telomerase activity, and induces apoptosis in human hepatoma-derived cell lines. [J] . Yasuda I, Shiratori Y, Adachi S, Journal of Hepatology: The Journal of the European Association for the Study of the Liver . 2002,第5期

机译：无环类维生素A诱导部分分化，下调端粒酶逆转录酶mRNA表达和端粒酶活性，并诱导人肝癌衍生的细胞系凋亡。
3. Effect of Lidamycin on Telomerase Activity in Human Hepatoma BEL-7402 Cells [J] . RUI-JUAN GAO, YUE-XIN LIANG, DIAN-DONG LI, 生物医学与环境科学（英文版） . 2007,第003期

机译：利达霉素对人肝癌BEL-7402细胞端粒酶活性的影响
4. DIAGNOSTIC VALUES OF COMBINED HEPATOMA-SPECIFIC α-FETOPROTEIN FRACTION AND TELOMERASE IN PATIENTS WITH HEPATOCELLULAR CARCINMA [C] . Wu Wei, Yao Dengfu, Qiu Liwei, Progress on post-genome technologies and modern natural products . 2011

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5. In vitro Evaluation of the Anti-cancer Potential of Miltirone in Human Hepatoma Cells. [D] . Zhou, Xuelin. 2012

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6. Telomerase Activity Detected by Quantitative Assay in Bladder Carcinoma and Exfoliated Cells in Urine [O] . Roberta Fedriga, Roberta Gunelli, Oriana Nanni, 2001

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7. Telomerase Activity Detected by Quantitative Assay in Bladder Carcinoma and Exfoliated Cells in Urine1 [O] . 2015

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8. Activity of Human Volunteer Sera to Candidate 'Plasmodium falciparum'Circumsporozoite Protein Vaccines in the Inhibition of Sporozoite Invasion Assay of Human Hepatoma Cells and Hepatocytes [R] . Hollingdale, M. R., Appiah, A., Leland, P., 1990

机译：人类志愿者血清对候选'恶性疟原虫'孢子孢子蛋白疫苗抑制人肝癌细胞和肝细胞子孢子入侵的活性

Using a non-radioisotopic, quantitative TRAP-based me- thod detecting telomerase activities in human hepatoma cells

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