...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell Research >RAPD analysis of natural populations of Acanthopanax brachypus
【24h】

RAPD analysis of natural populations of Acanthopanax brachypus

机译：刺五加自然种群的RAPD分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Random Amplified Polymorphic DNA (RAPD) analysis is a new technology of molecular marking which has proved very powerful in detecting genetic diversity at the level of population. The genomic DNAs used in our experiment were extracted from fresh leaves taken from 59 individuals sampled from three natural populations in Yan An, Shanxi Province. Through more than 2,000 PCRs, deep-going RAPD analysis was carried out on DNA samples from 49 inviduals. The percentage of polymorphic RAPD loci found in thesethree populations were respectively 27.2 %, 18.6 % and 5.4 %; the average genetic distances within population, 0.055, 0.036 and 0.008; the average genetic distances between populations (I-II), (I-III) and (II-III), 0.105, 0.096 and 0.060. The genetic diversity of A. brachypus within and between populations was found, for the first time, to be rather poor, thus revealing innate factors as the cause contributing to its endangered status. In addition, our work also provides basic materials for elucidatingthe underlying cause of its endangerment and for its protection biology.
机译：随机扩增多态性DNA（RAPD）分析是一种分子标记的新技术，已证明在检测种群水平的遗传多样性方面非常有效。我们的实验中使用的基因组DNA是从山西延安三个自然种群中采样的59个个体的新鲜叶片中提取的。通过2000多次PCR，对49位个体的DNA样本进行了深入的RAPD分析。在这三个种群中发现的多态性RAPD基因座的百分比分别为27.2％，18.6％和5.4％；群体内的平均遗传距离为0.055、0.036和0.008； （I-II），（I-III）和（II-III）群体之间的平均遗传距离为0.105、0.096和0.060。首次发现种群内部和种群之间的短枝曲霉的遗传多样性很差，因此揭示出先天因素是造成其濒危状态的原因。此外，我们的工作还为阐明其危害的根本原因及其保护生物学提供了基础材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号