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首页> 外文期刊>Cell Stress and Chaperones >Hsp27 (HSPB1): a possible surrogate molecular marker for loss of heterozygosity (LOH) of chromosome 1p in oligodendrogliomas but not in astrocytomas
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Hsp27 (HSPB1): a possible surrogate molecular marker for loss of heterozygosity (LOH) of chromosome 1p in oligodendrogliomas but not in astrocytomas

机译：Hsp27（HSPB1）：在少突胶质细胞瘤中而非星形细胞瘤中可能缺失1p号染色体的杂合性（LOH）的替代分子标记

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In oligodendrogliomas, 1p loss of heterozygosity (LOH) is a predictor of good prognosis and treatment response. In contrast, in uveal melanomas, LOH of chromosome 3 has been linked to poor prognosis and downregulation of Hsp27. In the present study, we have analyzed the expression of heat-shock proteins (Hsps) to characterize subtypes of gliomas and their histopathologic features and to correlate with other molecular markers including LOH of 1p. Biopsies from patients with primary gliomas (n = 65) were analyzed by immunohistochemistry, chromogenic in situ hybridization and fluorescent in situ hybridization and methylation-specific PCR (MSP). Elevated Hsp27 and total Hsp70 expression levels were associated with high-grade astrocytomas (p = 0.0001 and p = 0.01, respectively). In grade III oligodendrogliomas, the Hsp27 levels were significantly higher (p = 0.03). Low O6-methylguanine-DNA methyltransferase (MGMT) expression was associated with grade II astrocytomas. Elevated β-catenin expression was associated with grade III/IV astrocytomas (p = 0.003); p53 (+) tumors were more frequently found in grade III/IV astrocytomas (p = 0,001). LOH on 1p was associated with oligodendroglial tumours. In addition, a higher Hsp27 expression correlated with LOH of 1p (p = 0.017); this was also tested in two glioma cell lines. MSP was successful in only six samples. No significant correlations were found for the other markers. In conclusion, in oligodendroglial tumors, Hsp27 appeared as a surrogate marker of LOH of 1p which could also help to predict the disease prognosis. In gliomas, p53, Hsp27, Hsp70, MGMT, and β-catenin correlated with histopathological characteristics, suggesting that these markers could predict the disease outcome and the response to treatments.
机译：在少突胶质细胞瘤中，1p杂合性丧失（LOH）是预后和治疗反应良好的预测指标。相反，在葡萄膜黑色素瘤中，第3号染色体的LOH与Hsp27的不良预后和下调有关。在本研究中，我们分析了热休克蛋白（Hsps）的表达，以表征神经胶质瘤的亚型及其组织病理学特征，并与包括1p LOH在内的其他分子标记物相关联。通过免疫组织化学，显色原位杂交，荧光原位杂交和甲基化特异性PCR（MSP）对原发性神经胶质瘤（n = 65）患者的活检进行了分析。 Hsp27和总Hsp70表达水平升高与高度星形细胞瘤相关（分别为p = 0.0001和p = 0.01）。在III级少突胶质细胞瘤中，Hsp27水平明显更高（p = 0.03）。 O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）的低表达与II级星形细胞瘤相关。 β-catenin表达升高与III / IV级星形细胞瘤相关（p = 0.003）； p53（+）肿瘤在III / IV级星形细胞瘤中更为常见（p = 0,001）。 1p的LOH与少突胶质瘤有关。此外，较高的Hsp27表达与LOH为1p（p = 0.017）相关；这也在两个神经胶质瘤细胞系中进行了测试。 MSP仅在六个样本中成功。没有发现其他标记的显着相关性。总之，在少突神经胶质瘤中，Hsp27可以作为LOH为1p的替代标志物，也有助于预测疾病的预后。在神经胶质瘤中，p53，Hsp27，Hsp70，MGMT和β-catenin与组织病理学特征相关，表明这些标记物可以预测疾病的结果和对治疗的反应。

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