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首页> 外文期刊>Cell Stress and Chaperones >Triggers of the HSP70 stress response: environmental responses and laboratory manipulation in an Antarctic marine invertebrate (Nacella concinna)
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Triggers of the HSP70 stress response: environmental responses and laboratory manipulation in an Antarctic marine invertebrate (Nacella concinna)

机译：HSP70应激反应的触发因素：南极海洋无脊椎动物（Nacella concinna）的环境反应和实验室操作

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摘要

The Antarctic limpet, Nacella concinna, exhibits the classical heat shock response, with up-regulation of duplicated forms of the inducible heat shock protein 70 (HSP70) gene in response to experimental manipulation of seawater temperatures. However, this response only occurs in the laboratory at temperatures well in excess of any experienced in the field. Subsequent environmental sampling of inter-tidal animals also showed up-regulation of these genes, but at temperature thresholds much lower than those required to elicit a response in the laboratory. It was hypothesised that this was a reflection of the complexity of the stresses encountered in the inter-tidal region. Here, we describe a further series of experiments comprising both laboratory manipulation and environmental sampling of N. concinna. We investigate the expression of HSP70 gene family members (HSP70A, HSP70B, GRP78 and HSC70) in response to a further suite of environmental stressors: seasonal and experimental cold, freshwater, desiccation, chronic heat and periodic emersion. Lowered temperatures (?1.9°C and ?1.6°C), generally produced a down-regulation of all HSP70 family members, with some up-regulation of HSC70 when emerging from the winter period and increasing sea temperatures. There was no significant response to freshwater immersion. In response to acute and chronic heat treatments plus simulated tidal cycles, the data showed a clear pattern. HSP70A showed a strong but very short-term response to heat whilst the duplicated HSP70B also showed heat to be a trigger, but had a more sustained response to complex stresses. GRP78 expression indicates that it was acting as a generalised stress response under the experimental conditions described here. HSC70 was the major chaperone invoked in response to long-term stresses of varying types. These results provide intriguing clues not only to the complexity of HSP70 gene expression in response to environmental change but also insights into the stress response of a non-model species.
机译：南极帽贝Nacona continna表现出经典的热休克反应，响应于海水温度的实验控制，可诱导热休克蛋白70（HSP70）基因的重复形式上调。但是，此响应仅在实验室中的温度远远超过现场所经历的温度时发生。随后的潮间动物环境采样也显示了这些基因的上调，但其温度阈值比引起实验室反应所需的温度阈值低得多。假设这反映了潮间带遇到的压力的复杂性。在这里，我们描述了进一步的一系列实验，其中包括实验室操作和con。continna的环境采样。我们调查HSP70基因家族成员（HSP70A，HSP70B，GRP78和HSC70）的表达，以响应进一步的环境胁迫：季节性和实验性寒冷，淡水，干燥，慢性热和周期性出现。较低的温度（约1.9°C和约1.6°C）通常会导致所有HSP70家族成员的表达下调，而在冬季出现且海水温度升高时，HSC70也会上调。对淡水浸泡无明显反应。响应于急性和慢性热处理以及模拟潮汐周期，数据显示出清晰的模式。 HSP70A显示出对热的强烈但非常短期的响应，而复制的HSP70B也显示出热是触发因素，但对复杂的应力具有更持久的响应。 GRP78表达表明它在此处所述的实验条件下起普遍的应激反应作用。 HSC70是响应各种类型长期压力的主要伴侣蛋白。这些结果不仅为响应环境变化的HSP70基因表达的复杂性提供了有趣的线索，而且还为非模型物种的应激反应提供了见识。

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