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首页> 外文期刊>Cell and Tissue Research >Expression and role of receptor 1 for orexins in seminiferous tubules of rat testis
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Expression and role of receptor 1 for orexins in seminiferous tubules of rat testis

机译：食欲素受体1在大鼠睾丸生精小管中的表达及其作用

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Orexins (OxA and OxB) and their receptors (Ox1R and Ox2R), originally detected in the hypothalamus, have also been localized in multiple cerebral areas and peripheral organs. Thus, in addition to their central function in the regulation of food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual behaviour, arousal, and hypothalamic/hypophyseal axis, these neuropeptides may exert a local action in various peripheral organs and tissues. Emerging evidence suggests a main role of OxA and its highly specific receptor Ox1R in the male genital tract of mammals. We previously demonstrated OxA localization in Sertoli cells and spermatids of rat testis. Here, we show positive stainings of Ox1R in developing spermatocytes, and spermatids of rat testis by immunohistochemistry. The expression of Ox1R mRNA and the protein in the tissue was also established by reverse-transcription polymerase chain reaction and Western blotting respectively. The addition of OxA to fresh testis slices significantly increased testosterone (T) secretion which, conversely, was inhibited by Mullerian inhibiting substance (MIS). The sequential treatment of testis samples with the two substances highlighted an antagonizing activity of OxA versus MIS in regulating T levels. Furthermore, the stimulating effect on T production by OxA was prevented by the addition of the selective Ox1R inhibitor SB-408124. Overall, our findings suggest that locally secreted OxA interacting with Ox1R activates signals which antagonize MIS action in the control of T levels in mammalian testis.
机译：最初在下丘脑中发现的食欲素（OxA和OxB）及其受体（Ox1R和Ox2R）也已定位在多个大脑区域和周围器官中。因此，这些神经肽除了在调节食物摄入量，动脉血压，心率，睡眠/苏醒周期，性行为，唤醒和下丘脑/垂体干轴方面发挥中心作用外，还可能在各种外周器官和组织中发挥局部作用。组织。新兴证据表明，OxA及其高度特异性的受体Ox1R在哺乳动物的雄性生殖道中起主要作用。我们之前证明OxA定位在大鼠睾丸的支持细胞和精细胞中。在这里，我们通过免疫组织化学显示发育中的精母细胞和大鼠睾丸的精子中Ox1R的阳性染色。还通过逆转录聚合酶链反应和蛋白质印迹法分别确定了组织中Ox1R mRNA和蛋白的表达。向新鲜睾丸切片中添加OxA会显着增加睾丸激素（T）的分泌，反之，则被穆勒抑制物质（MIS）抑制。用这两种物质对睾丸样品进行的顺序处理突显了OxA对MIS在调节T水平方面的拮抗活性。此外，通过添加选择性Ox1R抑制剂SB-408124阻止了OxA对T产生的刺激作用。总体而言，我们的发现表明，局部分泌的OxA与Ox1R相互作用会激活信号，该信号在哺乳动物睾丸的T水平控制中拮抗MIS的作用。

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