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首页> 外文期刊>Cell and Tissue Research >Telocytes and putative stem cells in the lungs: electron microscopy, electron tomography and laser scanning microscopy
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Telocytes and putative stem cells in the lungs: electron microscopy, electron tomography and laser scanning microscopy

机译：肺中的卵母细胞和推定的干细胞：电子显微镜，电子断层扫描和激光扫描显微镜

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This study describes a novel type of interstitial (stromal) cell — telocytes (TCs) — in the human and mouse respiratory tree (terminal and respiratory bronchioles, as well as alveolar ducts). TCs have recently been described in pleura, epicardium, myocardium, endocardium, intestine, uterus, pancreas, mammary gland, etc. (see www.telocytes.com). TCs are cells with specific prolongations called telopodes (Tp), frequently two to three per cell. Tp are very long prolongations (tens up to hundreds of μm) built of alternating thin segments known as podomers (≤ 200 nm, below the resolving power of light microscope) and dilated segments called podoms, which accommodate mitochondria, rough endoplasmic reticulum and caveolae. Tp ramify dichotomously, making a 3-dimensional network with complex homo- and heterocellular junctions. Confocal microscopy reveals that TCs are c-kit- and CD34-positive. Tp release shed vesicles or exosomes, sending macromolecular signals to neighboring cells and eventually modifying their transcriptional activity. At bronchoalveolar junctions, TCs have been observed in close association with putative stem cells (SCs) in the subepithelial stroma. SCs are recognized by their ultrastructure and Sca-1 positivity. Tp surround SCs, forming complex TC-SC niches (TC-SCNs). Electron tomography allows the identification of bridging nanostructures, which connect Tp with SCs. In conclusion, this study shows the presence of TCs in lungs and identifies a TC-SC tandem in subepithelial niches of the bronchiolar tree. In TC-SCNs, the synergy of TCs and SCs may be based on nanocontacts and shed vesicles.
机译：这项研究描述了人类和小鼠呼吸树（末端和呼吸细支气管以及肺泡管）中的一种新型间质（基质）细胞— telocytes（TCs）。 TC在胸膜，心外膜，心肌，心内膜，肠，子宫，胰腺，乳腺等中已有描述（参见www.telocytes.com）。 TC是具有特定延伸的细胞，称为端粒（Tp），通常每个细胞2至3个。 Tp是非常长的延伸部分（数十到数百微米），由交替的细小片段（称为Podomer）组成（≤200 nm，低于光学显微镜的分辨力），而扩张的片段称为Podoms，可以容纳线粒体，粗糙的内质网和小窝。 Tp一分为二，形成具有复杂的同质和异质细胞连接的3维网络。共聚焦显微镜显示TCs是c-kit和CD34阳性的。 Tp释放会脱落小泡或外泌体，将大分子信号发送至邻近细胞，并最终改变其转录活性。在支气管肺泡连接处，已观察到TC与上皮下基质中的推定干细胞（SC）密切相关。 SC的超微结构和Sca-1阳性被认可。 Tp环绕SC，形成复杂的TC-SC壁ni（TC-SCN）。电子断层扫描可以识别桥接Tp与SC的纳米结构。总之，这项研究显示了TC在肺中的存在，并确定了TC-SC串联在支气管树上皮下位。在TC-SCN中，TC和SC的协同作用可能基于纳米接触和脱落的囊泡。

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