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首页> 外文期刊>Cell and Tissue Research >A novel collagen scaffold supports human osteogenesis—applications for bone tissue engineering
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A novel collagen scaffold supports human osteogenesis—applications for bone tissue engineering

机译：新型胶原蛋白支架支持人类成骨作用-在骨组织工程中的应用

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Collagen glycosaminoglycan (CG) scaffolds have been clinically approved as an application for skin regeneration. The goal of this study has been to examine whether a CG scaffold is a suitable biomaterial for generating human bone tissue. Specifically, we have asked the following questions: (1) can the scaffold support human osteoblast growth and differentiation and (2) how might recombinant human transforming growth factor-beta (TGF-β1) enhance long-term in vitro bone formation? We show human osteoblast attachment, infiltration and uniform distribution throughout the construct, reaching the centre within 14 days of seeding. We have identified the fully differentiated osteoblast phenotype categorised by the temporal expression of alkaline phosphatase, collagen type 1, osteonectin, bone sialo protein, biglycan and osteocalcin. Mineralised bone formation has been identified at 35 days post-seeding by using von Kossa and Alizarin S Red staining. Both gene expression and mineral staining suggest the benefit of introducing an initial high treatment of TGF-β1 (10 ng/ml) followed by a low continuous treatment (0.2 ng/ml) to enhance human osteogenesis on the scaffold. Osteogenesis coincides with a reduction in scaffold size and shape (up to 70% that of original). A notable finding is core degradation at the centre of the tissue-engineered construct after 49 days of culture. This is not observed at earlier time points. Therefore, a maximum of 35 days in culture is appropriate for in vitro studies of these scaffolds. We conclude that the CG scaffold shows excellent potential as a biomaterial for human bone tissue engineering.
机译：胶原蛋白糖胺聚糖（CG）支架已被临床批准用作皮肤再生的应用。这项研究的目的是检查CG支架是否适合用于生成人骨组织的生物材料。具体来说，我们提出了以下问题：（1）支架能否支持人类成骨细胞的生长和分化；（2）重组人类转化生长因子-β（TGF-β 1 ）如何增强长效性？术语体外骨形成？我们显示人类成骨细胞附着，渗透和均匀分布在整个构造，播种14天内到达中心。我们已经确定了完全分化的成骨细胞表型，按碱性磷酸酶，1型胶原蛋白，骨连接蛋白，骨唾液酸蛋白，双糖链蛋白和骨钙蛋白的时间表达分类。在播种后第35天，通过使用von Kossa和Alizarin S Red染色鉴定出矿化的骨形成。基因表达和矿物质染色均表明引入TGF-β 1 最初的高处理（10 ng / ml），然后进行低连续处理（0.2 ng / ml）以增强人类成骨的益处。脚手架。成骨与支架尺寸和形状的减少相吻合（最多可达原始支架尺寸的70％）。一个显着的发现是培养49天后，组织工程结构中心的核心降解。在较早的时间点未观察到此情况。因此，培养中最多35天适合于这些支架的体外研究。我们得出的结论是，CG支架作为人类骨组织工程的生物材料具有极好的潜力。

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