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Developmental changes in β-subunit composition of Na,K-ATPase in the Drosophila eye

机译：果蝇眼中Na，K-ATP酶β亚基组成的发育变化

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摘要

The Drosophila genome contains at least three loci for the Na,K-ATPase β-subunit; however, only the protein products of nrv1 and nrv2 have been characterized hitherto. Here, we provide evidence that nrv3 also encodes for a functional Na,K-ATPase β-subunit, as its protein product co-precipitates with the Na,K-ATPase α-subunit. Nrv3 expression in adult flies is restricted to the nervous system in which Nrv3 is enriched in selective types of sensory cells. Because Nrv3 expression is especially prominent in the compound eye, we have analyzed the subcellular and developmental distribution of Nrv3 within the visual cells and related this distribution to those of the α-subunit and of the β-subunits Nrv1 and Nrv2. Prospective visual cells express Nrv2 in the third larval instar stage and during the first half of pupal development. During the last third of pupal life, Nrv3 gradually replaces Nrv2 as the Na,K-ATPase β-subunit in the photoreceptor cells. Adult photoreceptors express Nrv3 as their major β-subunit; the visual cells R1–R6 co-express Nrv2 at a low level, whereas R7 and R8 co-express Nrv1. Notably, β-subunits do not co-distribute exactly with the α-subunit at some developmental stages, supporting the concept that the α-subunit and β-subunit can exist in the plasma membrane without being engaged in α/β heterodimers. The non-visual cells within the compound eye express almost exclusively Nrv2, which segregates together with the α-subunit to septate junctions throughout development.
机译：果蝇基因组包含至少三个Na，K-ATPaseβ亚基的基因座;然而，迄今为止，仅鉴定了nrv1和nrv2的蛋白质产物。在这里，我们提供的证据表明nrv3还编码功能性Na，K-ATPaseβ亚基，因为其蛋白质产物与Na，K-ATPaseα亚基共沉淀。 Nrv3在成年果蝇中的表达仅限于神经系统，其中Nrv3富含选择性类型的感觉细胞。由于Nrv3在复眼中的表达尤其突出，因此我们分析了Nrv3在视觉细胞内的亚细胞和发育分布，并将这种分布与α-亚基以及β-亚基Nrv1和Nrv2的分布相关。预期的视觉细胞在幼虫的第三龄期和of发育的前半期表达Nrv2。在p寿命的最后三分之一期间，Nrv3逐渐取代Nrv2，成为感光细胞中的Na，K-ATPaseβ亚基。成年感光细胞将Nrv3表达为其主要的β亚基。视觉细胞R1-R6共表达Nrv2，而R7和R8共表达Nrv1。值得注意的是，在某些发育阶段，β亚基不能与α亚基完全共分布，这支持了α亚基和β亚基可以不与α/β异二聚体结合而存在于质膜中的概念。复眼内的非视觉细胞几乎只表达Nrv2，它与α-亚基一起分离，从而在整个发育过程中分隔连接。

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