...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Developmental changes in beta-subunit composition of Na,K-ATPase in the Drosophila eye.
【24h】

Developmental changes in beta-subunit composition of Na,K-ATPase in the Drosophila eye.

机译：果蝇眼中Na，K-ATPase的β亚基组成的发育变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Drosophila genome contains at least three loci for the Na,K-ATPase beta-subunit; however, only the protein products of nrv1 and nrv2 have been characterized hitherto. Here, we provide evidence that nrv3 also encodes for a functional Na,K-ATPase beta-subunit, as its protein product co-precipitates with the Na,K-ATPase alpha-subunit. Nrv3 expression in adult flies is restricted to the nervous system in which Nrv3 is enriched in selective types of sensory cells. Because Nrv3 expression is especially prominent in the compound eye, we have analyzed the subcellular and developmental distribution of Nrv3 within the visual cells and related this distribution to those of the alpha-subunit and of the beta-subunits Nrv1 and Nrv2. Prospective visual cells express Nrv2 in the third larval instar stage and during the first half of pupal development. During the last third of pupal life, Nrv3 gradually replaces Nrv2 as the Na,K-ATPase beta-subunit in the photoreceptor cells. Adult photoreceptors express Nrv3 as their major beta-subunit; the visual cells R1-R6 co-express Nrv2 at a low level, whereas R7 and R8 co-express Nrv1. Notably, beta-subunits do not co-distribute exactly with the alpha-subunit at some developmental stages, supporting the concept that the alpha-subunit and beta-subunit can exist in the plasma membrane without being engaged in alpha/beta heterodimers. The non-visual cells within the compound eye express almost exclusively Nrv2, which segregates together with the alpha-subunit to septate junctions throughout development.
机译：果蝇基因组包含至少三个Na，K-ATPaseβ亚基的基因座;然而，迄今为止，仅鉴定了nrv1和nrv2的蛋白质产物。在这里，我们提供的证据表明，nrv3还编码功能性的Na，K-ATPaseβ-亚基，因为其蛋白质产物与Na，K-ATPaseα-亚基共沉淀。成年果蝇中的Nrv3表达仅限于神经系统，其中Nrv3富含选择性类型的感觉细胞。因为Nrv3的表达在复眼中尤其突出，所以我们分析了Nrv3在视觉细胞内的亚细胞和发育分布，并将这种分布与α-亚基和β-亚基Nrv1和Nrv2的分布相关。预期的视觉细胞在幼虫的第三龄期和of发育的前半期表达Nrv2。在p寿命的最后三分之一期间，Nrv3逐渐取代Nrv2，成为感光细胞中的Na，K-ATPaseβ亚基。成年感光细胞将Nrv3表达为其主要的β亚基。视觉单元格R1-R6共表达Nrv2，而R7和R8共表达Nrv1。值得注意的是，β-亚基在某些发育阶段不能与α-亚基完全共分布，这支持了α-亚基和β-亚基可以存在于质膜中而不参与α/β异二聚体的概念。复眼内的非视觉细胞几乎只表达Nrv2，它与α亚基一起分离，从而在整个发育过程中分隔连接。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号