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首页> 外文期刊>Cell and Tissue Research >New insights into autophagic cell death in the gypsy moth Lymantria dispar: a proteomic approach
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New insights into autophagic cell death in the gypsy moth Lymantria dispar: a proteomic approach

机译：吉普赛蛾Lymantria dispar中自噬细胞死亡的新见解：蛋白质组学方法

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Autophagy is an evolutionary ancient process based on the activity of genes conserved from yeast to metazoan taxa. Whereas its role as a mechanism to provide energy during cell starvation is commonly accepted, debate continues about the occurrence of autophagy as a means specifically activated to achieve cell death. The IPLB-LdFB insect cell line, derived from the larval fat body of the lepidoptera Lymantria dispar, represents a suitable model to address this question, as both autophagic and apoptotic cell death can be induced by various stimuli. Using morphological and functional approaches, we have observed that the culture medium conditioned by IPLB-LdFB cells committed to death by the ATPase inhibitor oligomycin A stimulates autophagic cell death in untreated IPLB-LdFB cells. Moreover, proteomic analysis of the conditioned media suggests that, in IPLB-LdFB cells, oligomycin A promotes a shift towards lipid metabolism, increases oxidative stress and specifically directs the cells towards autophagic activity.
机译：自噬是一个古老的进化过程，它基于从酵母到后生类群保守基因的活性。尽管人们普遍接受其作为在细胞饥饿期间提供能量的机制的作用，但关于自噬作为特定激活的手段来实现细胞死亡的手段的争论仍在继续。 IPLB-LdFB昆虫细胞系来源于鳞翅目鳞翅目幼虫的幼体脂肪，它代表了解决这一问题的合适模型，因为自噬和凋亡细胞死亡均可通过各种刺激来诱导。使用形态学和功能方法，我们已经观察到由IPLB-LdFB细胞条件化并由ATPase抑制剂寡霉素A致死的培养基刺激了未经处理的IPLB-LdFB细胞的自噬细胞死亡。此外，对条件培养基的蛋白质组学分析表明，在IPLB-LdFB细胞中，寡霉素A促进了脂质代谢的转变，增加了氧化应激，并特别指导细胞实现自噬活性。

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