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首页> 外文期刊>Cell and Tissue Research >Identification of neuron types in the submucosal ganglia of the mouse ileum
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Identification of neuron types in the submucosal ganglia of the mouse ileum

机译：小鼠回肠粘膜下神经节中神经元类型的鉴定

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The continuing and even expanding use of genetically modified mice to investigate the normal physiology and development of the enteric nervous system and for the study of pathophysiology in mouse models emphasises the need to identify all the neuron types and their functional roles in mice. An investigation that chemically and morphologically defined all the major neuron types with cell bodies in myenteric ganglia of the mouse small intestine was recently completed. The present study was aimed at the submucosal ganglia, with the purpose of similarly identifying the major neuron types with cell bodies in these ganglia. We found that the submucosal neurons could be divided into three major groups: neurons with vasoactive intestinal peptide (VIP) immunoreactivity (51% of neurons), neurons with choline acetyltransferase (ChAT) immunoreactivity (41% of neurons) and neurons that expressed neither of these markers. Most VIP neurons contained neuropeptide Y (NPY) and about 40% were immunoreactive for tyrosine hydroxylase (TH); 22% of all submucosal neurons were TH/VIP. VIP-immunoreactive nerve terminals in the mucosa were weakly immunoreactive for TH but separate populations of TH- and VIP-immunoreactive axons innervated the arterioles in the submucosa. Of the ChAT neurons, about half were immunoreactive for both somatostatin and calcitonin gene-related peptide (CGRP). Calretinin immunoreactivity occurred in over 90% of neurons, including the VIP neurons. The submucosal ganglia and submucosal arterioles were innervated by sympathetic noradrenergic neurons that were immunoreactive for TH and NPY; no VIP and few calretinin fibres innervated submucosal neurons. We conclude that the submucosal ganglia contain cell bodies of VIP/NPY/TH/calretinin non-cholinergic secretomotor neurons, VIP/NPY/calretinin vasodilator neurons, ChAT/CGRP/somatostatin/calretinin cholinergic secretomotor neurons and small populations of cholinergic and non-cholinergic neurons whose targets have yet to be identified. No evidence for the presence of type-II putative intrinsic primary afferent neurons was found.
机译：转基因小鼠的持续乃至扩大应用，以研究肠道神经系统的正常生理学和发育，以及在小鼠模型中研究病理生理学，这强调了确定小鼠中所有神经元类型及其功能作用的需要。最近完成了一项化学和形态学研究，确定了小鼠小肠肌层神经节中所有主要神经元类型与细胞体的关系。本研究针对粘膜下神经节，目的是类似地识别这些神经节中具有细胞体的主要神经元类型。我们发现粘膜下神经元可以分为三大类：具有血管活性肠肽（VIP）免疫反应性的神经元（占神经元的51％），具有胆碱乙酰转移酶（ChAT）免疫反应性的神经元（占41％的神经元）和不表达两者的神经元这些标记。大多数VIP神经元含有神经肽Y（NPY），约40％对酪氨酸羟化酶（TH）具有免疫反应性。所有粘膜下神经元的22％是TH / VIP。粘膜中VIP免疫反应性神经末梢对TH的免疫反应较弱，但TH和VIP免疫反应性轴突的单独种群支配了粘膜下层的小动脉。在ChAT神经元中，约有一半对生长抑素和降钙素基因相关肽（CGRP）具有免疫反应性。 Calretinin免疫反应性发生在90％以上的神经元中，包括VIP神经元。粘膜下神经节和粘膜下小动脉被交感性去甲肾上腺素能神经元支配，对TH和NPY具有免疫反应性。没有VIP和很少的钙网蛋白纤维支配粘膜下神经元。我们得出的结论是，粘膜下神经节包含VIP / NPY / TH / calretinin非胆碱能分泌运动神经元，VIP / NPY / calretinin血管扩张神经元，ChAT / CGRP / somatostatin / calretinin胆碱能分泌运动神经元和少量胆碱能和非胆碱能神经元的细胞体其目标尚未确定的神经元。没有证据表明存在II型推定的固有原代传入神经元。

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