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首页> 外文期刊>Cell and Tissue Research >Immunolocalization of α1A-adrenoceptors in rat and human epididymis
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Immunolocalization of α1A-adrenoceptors in rat and human epididymis

机译：大鼠和人附睾中α1A-肾上腺素受体的免疫定位

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摘要

Immunohistochemistry was conducted to analyze the cellular localization of α1A-adrenoceptors along rat and human epididymis. ADR-A, a polyclonal antibody that recognizes the specific C-terminal region of α1A-adrenoceptors, immunostained this adrenoceptor subtype in smooth muscle cells surrounding the epididymal tubules and interstitial blood vessels and in subpopulations of epithelial cells from adult rat and human caput and cauda epididymidis. The same cell types from rat epididymidis were immunostained by ADR-1, a polyclonal antibody that recognizes a common region of the three α1-adrenoceptor subtypes, α1A, α1B, and α1D. Immunostaining with both antibodies was also conducted in adult rat and human vas deferens and seminal vesicle used as positive controls because of the abundance of α1A-adrenoceptors in these tissues. ADR-A- and ADR-1-positive immunostaining was differentially distributed depending on the antibody, method of tissue fixation (Bouin-fixed and fresh frozen tissues), species (rat and human), tissue (caput and cauda epididymidis), and age (immature and adult rats) analyzed. This is the first report immunolocalizing α1A-adrenoceptor along rat and human epididymis. The presence of this adrenoceptor subtype in epididymal smooth muscle and epithelial cells indicates their contribution to smooth muscle contractile responses and a possible role in the absorptive and/or secretory activities of the epithelium lining the epididymal duct. Taken together, our results should contribute to a better understanding of the physiological role of α1-adrenoceptors in the epididymidis and the importance of the sympathetic nervous system for male (in)fertility.
机译：免疫组织化学分析了α1A-肾上腺素受体在大鼠和人附睾中的细胞定位。 ADR-A是一种识别α1A-肾上腺素能受体特定C端区域的多克隆抗体，在成年大鼠和上皮小管周围的平滑肌细胞和上皮细胞亚群中免疫染色这种肾上腺素能受体亚型。人参和附睾。用多克隆抗体ADR-1对大鼠附睾上的相同细胞类型进行免疫染色，该多克隆抗体可识别三种α1-肾上腺素受体亚型，α1A，α1B和α1D的共同区域。子>。在成年大鼠和人输精管中也使用了这两种抗体进行免疫染色，并且由于这些组织中存在大量的α1A-肾上腺素受体，因此将精囊用作阳性对照。 ADR-A-和ADR-1阳性免疫染色的分布取决于抗体，组织固定方法（鲍恩固定和新鲜冷冻组织），物种（大鼠和人），组织（附睾和附睾）和年龄（未成熟和成年大鼠）进行了分析。这是第一个关于大鼠和人附睾免疫定位α1A-肾上腺素受体的报道。附睾平滑肌和上皮细胞中这种肾上腺素能受体亚型的存在表明它们对平滑肌的收缩反应作出了贡献，并可能在上皮导管上皮的吸收和/或分泌活动中发挥了作用。两者合计，我们的结果应有助于更好地了解附睾中α1-肾上腺素受体的生理作用以及交感神经系统对男性（不育）的重要性。

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  • 来源
    《Cell and Tissue Research》 |2008年第3期|509-522|共14页
  • 作者

    Daniel Barboza Cava Queiróz; Catarina S. Porto; Gail Grossman; Peter Petrusz; Maria Christina W. Avellar;

  • 作者单位

    Section of Experimental Endocrinology Department of Pharmacology Universidade Federal de São Paulo - Escola Paulista de Medicina Rua 3 de maio 100 INFAR Vila Clementino São Paulo 04044-020 Brazil;

    Section of Experimental Endocrinology Department of Pharmacology Universidade Federal de São Paulo - Escola Paulista de Medicina Rua 3 de maio 100 INFAR Vila Clementino São Paulo 04044-020 Brazil;

    Department of Cell and Developmental Biology and Pediatrics Laboratories for Reproductive Biology University of North Carolina Chapel Hill NC 27599-7500 USA;

    Department of Cell and Developmental Biology and Pediatrics Laboratories for Reproductive Biology University of North Carolina Chapel Hill NC 27599-7500 USA;

    Section of Experimental Endocrinology Department of Pharmacology Universidade Federal de São Paulo - Escola Paulista de Medicina Rua 3 de maio 100 INFAR Vila Clementino São Paulo 04044-020 Brazil;

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  • 关键词

    Epididymidis; Adrenoceptor; Male reproductive tract; Immunohistochemistry; Rat (Wistar); Human;

    机译：附睾;肾上腺素受体;男性生殖道;免疫组织化学;大鼠（Wistar）;人类;

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