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首页> 外文期刊>Cell and Tissue Research >Expression of Sox2 in mouse taste buds and its relation to innervation
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Expression of Sox2 in mouse taste buds and its relation to innervation

机译：Sox2在小鼠味蕾中的表达及其与神经支配的关系

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Sox2, which encodes an HMG box transcription factor, is known to regulate the differentiation of progenitor cells of the tongue into taste bud cells versus keratinocytes during development. To determine the neural dependence of Sox2 expression, glossopharyngeal nerves of mice were cut bilaterally. In unoperated mice, the expression of Sox2 mRNA and protein was restricted to a subset of taste bud cells and to the epithelium surrounding the taste buds of the circumvallate papillae. During the period of denervation, the taste buds largely disappeared; the taste bud cells and the epithelial cells with Sox2-immunoreactive (IR) nuclei decreased in number and totally disappeared from the epithelium by 16 days after denervation. When regenerated nerve fibers entered the epithelium, Sox2 expression reappeared, first in the epithelial cells, and then in the regenerating taste bud cells. In prenatal mice, Sox2 was expressed in the epithelium of the dorsal surface of circumvallate papillae, in regions into which numerous nerve fibers had entered. The results suggested that Sox2 expression was dependent on gustatory innervation. Sox2-IR cells in the taste buds were also examined by double-immunolabeling for 5-bromo-2′-deoxyuridine and cell-type markers such as cytokeratin 14, neural cell adhesion molecule, inositol 1,4,5-triphosphate receptor 3, and blood group H antigen. Sox2-IR cells were found in the populations of basal cells and of immature and some mature taste bud cells. A large number of Sox2-IR cells were identified as type-I cells, with a few being type-II and type-III cells.
机译：已知编码HMG框转录因子的Sox2在发育过程中可调节舌头祖细胞向味蕾细胞与角质形成细胞的分化。为了确定Sox2表达的神经依赖性，双侧切除小鼠的舌咽神经。在未经手术的小鼠中，Sox2 mRNA和蛋白质的表达仅限于味蕾细胞的一个子集，以及周围的乳突乳头的味蕾周围的上皮细胞。去神经期间，味蕾基本上消失了。去神经后16天，具有Sox2免疫反应（IR）核的味蕾细胞和上皮细胞数量减少，并从上皮中完全消失。当再生的神经纤维进入上皮时，Sox2表达重新出现，首先出现在上皮细胞中，然后出现在再生的味蕾细胞中。在产前小鼠中，Sox2在环状神经乳头背面的上皮中，在其中许多神经纤维进入的区域中表达。结果表明Sox2表达取决于味觉神经支配。还通过双重免疫标记5-溴2'-脱氧尿苷和细胞类型标记（例如细胞角蛋白14，神经细胞粘附分子，肌醇1,4,5-三磷酸受体3，和血型H抗原。在基础细胞，未成熟和一些成熟味蕾细胞中发现了Sox2-IR细胞。大量的Sox2-IR细胞被鉴定为I型细胞，少数是II型和III型细胞。

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