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Collagen synthesis of articular cartilage explants in response to frequency of cyclic mechanical loading

机译：响应循环机械负荷频率的关节软骨外植体胶原蛋白合成

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摘要

Articular cartilage in vivo experiences the effects of both cell-regulatory proteins and mechanical forces. This study has addressed the hypothesis that the frequency of intermittently or continuously applied mechanical loads is a critical parameter in the regulation of chondrocyte collagen biosynthesis. Cyclic compressive pressure was applied intermittently to bovine articular cartilage explants by using a sinusoidal waveform of 0.1–1.0 Hz frequency with a peak stress of 0.5 MPa for a period of 5–20 s followed by a load-free period of 10–1,000 s. These loading protocols were repeated for a total duration of 6 days. In separate experiments, cyclic loading was continuously applied by using a sinusoidal waveform of 0.001–0.5 Hz frequency and a peak stress of 1.0 MPa for a period of 3 days. Unloaded cartilage discs of the same condyle were cultured in identically constructed loading chambers and served as controls. We report quantitative data showing that (1) no correlation exists between the relative rate of collagen synthesis expressed as the proportion of newly synthesized collagen among newly made proteins and either the frequency of intermittently or continuously applied loads or the overall time cartilage is actively loaded, and (2) individual protocols of intermittently applied loads can reduce the relative rate of collagen synthesis and increase the water content, whereas (3) continuously applied cyclic loads always suppress the relative rate of collagen synthesis compared with that of unloaded control specimens. The results provide further experimental evidence that collagen metabolism is difficult to manipulate by mechanical stimuli. This is physiologically important for the maintainance of the material properties of collagen in view of the heavy mechanical demands made upon it. Moreover, the unaltered or reduced collagen synthesis of cartilage explants might reflect more closely the metabolism of normal or early human osteoarthritic cartilage.
机译：体内关节软骨会同时受细胞调节蛋白和机械力的影响。这项研究解决了以下假设：间歇或连续施加的机械负荷的频率是调节软骨细胞胶原生物合成的关键参数。通过使用频率为0.1–1.0 Hz的正弦波形，峰值应力为0.5 MPa的正弦波形，在5–20 s内间歇性地对牛关节软骨外植体施加周期性压缩压力，持续10–1,000 s的时间。重复这些加载方案，总共持续6天。在单独的实验中，通过使用0.001–0.5 Hz频率的正弦波形和1.0 MPa的峰值应力连续施加循环载荷3天。在相同构造的装载室中培养相同con的未装载软骨盘，并用作对照。我们报告了定量数据，这些数据表明（1）胶原蛋白合成的相对速率（以新合成的胶原蛋白在新制成的蛋白质中所占的比例表示）与间歇或连续施加的载荷的频率或活跃的总软骨时间之间不存在相关性， （2）间歇施加负荷的个别方案可以降低胶原蛋白合成的相对速率并增加水含量，而（3）连续施加循环载荷与空载对照样品相比，总是会抑制胶原蛋白的相对速率。该结果提供了进一步的实验证据，表明胶原蛋白的代谢难以通过机械刺激来操纵。鉴于对胶原蛋白的大量机械需求，这对于维持胶原蛋白的材料特性在生理上是重要的。而且，软骨外植体的胶原合成未改变或减少可能更紧密地反映了正常或早期人类骨关节炎软骨的代谢。

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  • 来源
    《Cell and Tissue Research》 |2007年第1期|155-166|共12页
  • 作者

    Amela Wolf; Beate Ackermann; Jürgen Steinmeyer;

  • 作者单位

    Orthopaedic Research Laboratories Department of Orthopaedic Surgery University Clinics Giessen and Marburg Paul-Meimberg-Strasse 3 35385 Giessen Germany;

    Orthopaedic Research Laboratories Department of Orthopaedic Surgery University Clinics Giessen and Marburg Paul-Meimberg-Strasse 3 35385 Giessen Germany;

    Orthopaedic Research Laboratories Department of Orthopaedic Surgery University Clinics Giessen and Marburg Paul-Meimberg-Strasse 3 35385 Giessen Germany;

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  • 正文语种 eng
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  • 关键词

    Collagen; Proteins; Cartilage; Biomechanics; Osteoarthritis; Bovine;

    机译：胶原蛋白;软骨;生物力学;骨关节炎;牛;

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