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首页> 外文期刊>Cell and Tissue Research >Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle
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Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle

机译：大鼠骨髓，滑膜，骨膜，脂肪组织和肌肉间充质干细胞的比较

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摘要

Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of experiments in vivo. To determine a suitable cell source, we isolated rat MSCs from bone marrow, synovium, periosteum, adipose, and muscle and compared their properties for yield, expansion, and multipotentiality. After two passages, the cells in each population were CD11b (?), CD45 (?), and CD90 (+). The colony number per nucleated cells derived from synovium was 100-fold higher than that for cells derived from bone marrow. With regard to expansion potential, synovium-derived cells were the highest in colony-forming efficiency, fold increase, and growth kinetics. An in vitro chondrogenesis assay demonstrated that the pellets derived from synovium were heavier, because of their greater production of cartilage matrix, than those from other tissues, indicating their superiority in chondrogenesis. Synovium-derived cells retained their chondrogenic potential after a few passages. The Oil Red-O positive colony-rate assay demonstrated higher adipogenic potential in synovium- and adipose-derived cells. Alkaline phosphatase activity was greater in periosteum- and muscle-derived cells during calcification. The yield and proliferation potential of rat MSCs from solid tissues was much better than those from bone marrow. In particular, synovium-derived cells had the greatest potential for both proliferation and chondrogenesis, indicating their usefulness for cartilage study in a rat model.
机译：间充质干细胞（MSCs）越来越多地被报道存在于各种组织中。尽管从人骨髓中提取MSC相对容易，但是分离啮齿类MSC却更加困难，从而限制了体内实验的数量。为了确定合适的细胞来源，我们从骨髓，滑膜，骨膜，脂肪和肌肉中分离了大鼠MSC，并比较了它们的产量，扩增和多潜能特性。经过两次传代后，每个群体中的细胞分别是CD11b（？），CD45（？）和CD90（+）。滑膜来源的每个有核细胞的菌落数比骨髓来源的细胞高100倍。关于扩增潜力，滑膜来源的细胞在菌落形成效率，倍数增加和生长动力学方面最高。体外软骨形成试验表明，滑膜来源的沉淀物比其他组织的沉淀物重，因为它们的软骨基质产量更高，这表明它们在软骨形成方面具有优势。滑膜衍生细胞经过几代后仍保留了其成软骨潜力。 Oil Red-O阳性菌落率测定表明滑膜和脂肪来源的细胞具有更高的成脂潜能。钙化过程中，骨膜和肌肉来源的细胞中碱性磷酸酶活性较高。来自实体组织的大鼠MSC的产量和增殖潜能要比来自骨髓的MSC高得多。特别是，滑膜来源的细胞具有最大的增殖和软骨形成潜能，表明它们对大鼠模型软骨研究有用。

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