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首页> 外文期刊>Cell and Tissue Research >Raver1 is an integral component of muscle contractile elements
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Raver1 is an integral component of muscle contractile elements

机译：Raver1是肌肉收缩元素的组成部分

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摘要

Raver1, a ubiquitously expressed protein, was originally identified as a ligand for metavinculin, the muscle-specific isoform of the microfilament-associated protein vinculin. The protein resides primarily in the nucleus, where it colocalises and may interact with polypyrimidine-tract-binding protein, which is involved in alternative splicing processes. During skeletal muscle differentiation, raver1 translocates to the cytoplasm and eventually targets the Z-line of sarcomeres. Here, it colocalises with metavinculin, vinculin and alpha-actinin, all of which have biochemically been identified as raver1 ligands. To obtain more information about the potential role of raver1 in muscle structure and function, we have investigated its distribution and fine localisation in mouse striated and smooth muscle, by using three monoclonal antibodies that recognise epitopes in different regions of the raver1 protein. Our immunofluorescence and immunoelectron-microscopic results indicate that the cytoplasmic accumulation of raver1 is not confined to skeletal muscle but also occurs in heart and smooth muscle. Unlike vinculin and metavinculin, cytoplasmic raver1 is not restricted to costameres but additionally represents an integral part of the sarcomere. In isolated myofibrils and in ultrathin sections of skeletal muscle, raver1 has been found concentrated at the I-Z-I band. A minor fraction of raver1 is present in the nuclei of all three types of muscle. These data indicate that, during muscle differentiation, raver1 might link gene expression with structural functions of the contractile machinery of muscle.
机译：Raver1是一种普遍表达的蛋白质，最初被确定为metavinculin的配体，metavinculin是与微丝相关的蛋白纽蛋白的肌肉特异性同种型。该蛋白质主要位于细胞核中，在该细胞中共定位并可能与聚嘧啶束结合蛋白相互作用，后者参与其他剪接过程。在骨骼肌分化过程中，raver1易位至细胞质，并最终靶向肉瘤的Z线。在这里，它与metavinculin，vincinin和α-actinin共定位，所有这些在生化上都被鉴定为raver1配体。为了获得有关raver1在肌肉结构和功能中潜在作用的更多信息，我们通过使用识别raver1蛋白不同区域表位的三种单克隆抗体，研究了其在小鼠横纹和平滑肌中的分布和精细定位。我们的免疫荧光和免疫电子显微镜结果表明，raver1的细胞质积聚不仅限于骨骼肌，而且还存在于心脏和平滑肌中。与新蛋白和metavinculin不同，胞质raver1不仅限于肋骨，而是肌节的组成部分。在分离的肌原纤维和骨骼肌超薄切片中，raver1被发现集中在I-Z-I谱带。在所有三种类型的肌肉的核中都存在少量的raver1。这些数据表明，在肌肉分化过程中，raver1可能将基因表达与肌肉收缩机制的结构功能联系起来。

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  • 来源
    《Cell and Tissue Research》 |2007年第3期|583-594|共12页
  • 作者

    Anke Zieseniss; Ulrich Schroeder; Sabine Buchmeier; Cora-Ann Schoenenberger; Joop van den Heuvel; Brigitte M. Jockusch; Susanne Illenberger;

  • 作者单位

    Cell Biology Zoological Institute Technical University of Braunschweig Biocentre Spielmannstrasse 7 38092 Braunschweig Germany;

    Maurice E. Mueller Institute for Structural Biology Biozentrum University of Basel CH-4056 Basel Switzerland;

    Cell Biology Zoological Institute Technical University of Braunschweig Biocentre Spielmannstrasse 7 38092 Braunschweig Germany;

    Maurice E. Mueller Institute for Structural Biology Biozentrum University of Basel CH-4056 Basel Switzerland;

    Division of Structural Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany;

    Cell Biology Zoological Institute Technical University of Braunschweig Biocentre Spielmannstrasse 7 38092 Braunschweig Germany;

    Cell Biology Zoological Institute Technical University of Braunschweig Biocentre Spielmannstrasse 7 38092 Braunschweig Germany;

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  • 正文语种 eng
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  • 关键词

    hnRNP; Striated muscle; Smooth muscle; Sarcomere; Myofibril; Mouse;

    机译：hnRNP;横纹肌;平滑肌;Sarcomere;肌原纤维;小鼠;

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