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首页> 外文期刊>Cell and Tissue Research >Calcitonin is expressed in the chicken pituitary gland: influence of gonadal steroids and sexual maturation
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Calcitonin is expressed in the chicken pituitary gland: influence of gonadal steroids and sexual maturation

机译：降钙素在鸡的垂体中表达：性腺类固醇和性成熟的影响

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Calcitonin (CT) is primarily produced by the thyroid C cells in mammals or by the ultimobranchial gland in chickens. CT is also expressed by the pituitary gland in rats in which it functions as a paracrine factor causing decreased lactotroph proliferation and prolactin (PRL) secretion. Gonadal steroids influence CT expression in the rat pituitary gland. However, the expression of the CT gene in the pituitary gland of chickens or of any other avian species has not previously been reported. We have tested the hypotheses that CT is expressed in the chicken pituitary gland, and that its expression is influenced by sexual maturation or in response to ovarian steroid administration. We have detected robust expression of CT cDNA in the chicken pituitary gland by reverse transcription/polymerase chain reaction (PCR). The sequence of the pituitary-derived CT cDNA is identical to that of the ultimobranchial gland. CT-immunoreactive (ir) cells have been observed throughout the anterior pituitary gland by confocal microscopy. Many of the PRL-ir cells show co-localization with CT-ir cells. Quantitative real-time PCR analysis has revealed an inverse relationship between the quantities of PRL mRNA and CT mRNA in the pituitary gland: sexually mature hens contain lower amounts of CT mRNA but larger quantities of PRL mRNA compared with sexually immature chickens. Estradiol and/or progesterone treatment of sexually immature chickens leads to a significant decrease in the quantity of pituitary CT mRNA relative to that in the vehicle-treated chickens. We conclude that pituitary CT plays an important paracrine/autocrine role in the control of lactotroph function and PRL secretion in the chicken.
机译：降钙素（CT）主要由哺乳动物的甲状腺C细胞或鸡的支气管腺产生。垂体CT在大鼠的脑垂体中也表达，它起旁分泌因子的作用，引起乳营养生长和催乳素（PRL）分泌减少。性腺类固醇会影响大鼠垂体CT的表达。但是，以前尚未报道过CT基因在鸡或任何其他禽类的垂体中的表达。我们已经测试了CT在鸡垂体中表达，并且其表达受性成熟或对卵巢类固醇激素反应的影响的假设。我们已经通过逆转录/聚合酶链反应（PCR）检测到鸡垂体中CT cDNA的表达。垂体CT cDNA的序列与支气管腺的序列相同。共聚焦显微镜在整个垂体前叶观察到CT免疫反应（ir）细胞。许多PRL-ir细胞显示与CT-ir细胞共定位。实时定量PCR分析显示，垂体腺中PRL mRNA和CT mRNA的数量呈反比关系：与性未成熟的鸡相比，有性成熟的母鸡的CT mRNA含量较低，但PRL mRNA的含量较高。雌二醇和/或孕激素治疗性不成熟鸡会导致垂体CT mRNA量相对于运载体治疗鸡而言显着减少。我们得出的结论是，垂体CT在控制鸡的营养性功能和PRL分泌中起着重要的旁分泌/自分泌作用。

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