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首页> 外文期刊>Cell and Tissue Research >Differential distribution of adenosine receptors in rat cochlea
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Differential distribution of adenosine receptors in rat cochlea

机译：大鼠耳蜗中腺苷受体的差异分布

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Adenosine is a constitutive cell metabolite that can be released from cells via specific bi-directional transporters and is an end-point for nucleotide hydrolysis. In the extracellular space, adenosine becomes a signalling molecule for P1 (adenosine) receptors that modulate physiological responses in a wide range of mammalian tissues. Whereas adenosine signalling has been implicated in the regulation of cochlear blood flow and in cochlear protection from oxidative damage, the potential roles for adenosine signalling in the modulation of sound transduction and auditory neurotransmission have not been established. We have characterised the expression and distribution of adenosine receptors in the rat cochlea. mRNA transcripts for all four subtypes of adenosine receptors (A1, A2A, A2B and A3) were detected in dissected cochlear tissue by using reverse transcription/polymerase chain reaction analysis. The protein distribution for the A1, A2A and A3 receptor subtypes was identified by immunoperoxidase histochemistry and confocal immunofluorescence labelling. These receptors were differentially expressed in the organ of Corti, spiral ganglion neurones, lateral wall tissues and cochlear blood vessels. The distribution of adenosine receptors in sensory and neural tissues and in the vasculature coincided with other elements of purinergic signalling (P2X and P2Y receptors, ectonucleotidases), consistent with the integrative regulation of many physiological processes in the cochlea by extracellular nucleotides and nucleosides. Our study provides a framework for further investigation of adenosine signalling in the inner ear, including putative roles in oxidative stress responses.
机译：腺苷是一种组成型细胞代谢产物，可以通过特定的双向转运蛋白从细胞中释放出来，并且是核苷酸水解的终点。在细胞外空间中，腺苷成为P1（腺苷）受体的信号传导分子，可调节多种哺乳动物组织中的生理反应。尽管腺苷信号传导参与了耳蜗血流的调节以及对耳蜗的氧化损伤的保护作用，但尚未确定腺苷信号传导在调节声音传导和听觉神经传递中的潜在作用。我们已经表征了大鼠耳蜗中腺苷受体的表达和分布。通过逆转录/聚合酶链反应分析，在解剖后的耳蜗组织中检测出腺苷受体的四种亚型（A1 ，A2A ，A2B 和A3 ）的mRNA转录物。通过免疫过氧化物酶组织化学和共聚焦免疫荧光标记鉴定了A1 ，A2A 和A3 受体亚型的蛋白质分布。这些受体在Corti，螺旋神经节神经元，侧壁组织和耳蜗血管的器官中差异表达。腺苷受体在感觉和神经组织以及脉管系统中的分布与嘌呤能信号传导的其他元素（P2X和P2Y受体，胞外核苷酸酶）相吻合，这与耳蜗中细胞外核苷酸和核苷对许多生理过程的整体调节一致。我们的研究为进一步研究内耳的腺苷信号提供了框架，包括在氧化应激反应中的假定作用。

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