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首页> 外文期刊>Cell and Tissue Research >Peptide immunocytochemistry of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae
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Peptide immunocytochemistry of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae

机译：吹蝇突触到脑后复合体的神经元的肽免疫细胞化学

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摘要

Antisera against a variety of vertebrate and invertebrate neuropeptides were used to characterize neurons with somata in the pars intercerebralis (PI), pars lateralis (PL), and subesophageal ganglion (SEG), designated as PI neurons, PL neurons, and SEG neurons, respectively, all of which project to the retrocerebral complex in the blow fly, Protophormia terraenovae. Immunocytochemistry combined with backfills through the cardiac-recurrent nerve revealed that at least two pairs of PI and SEG neurons for each were FMRFamide-immunoreactive. Immunoreactivity against [Arg7]-corazonin, β-pigment-dispersing hormone (β-PDH), cholecystokinin8, or FMRFamide was observed in PL neurons. Immunoreactive colocalization of [Arg7]-corazonin with β-PDH, [Arg7]-corazonin with cholecystokinin8, or β-PDH with FMRFamide was found in two to three somata in the PL of a hemisphere. Based on their anatomical and immunocytochemical characteristics, PI neurons were classified into two types, PL neurons into six types, and SEG neurons into two types. Fibers in the retrocerebral complex showed [Arg7]-corazonin, β-PDH, cholecystokinin8, and FMRFamide immunoreactivity. Cholecystokinin8 immunoreactivity was also detected in intrinsic cells of the corpus cardiacum. The corpus allatum was densely innervated by FMRFamide-immunoreactive varicose fibers. These results suggest that PI, PL, and SEG neurons release [Arg7]-corazonin, β-PDH, cholecystokinin8, or FMRFamide-like peptides from the corpus cardiacum or corpus allatum into the hemolymph, and that some PL neurons may simultaneously release several neuropeptides.
机译：针对各种脊椎动物和无脊椎动物神经肽的抗血清被用来表征大脑皮层（PI），侧脑皮层（PL）和食管下神经节（SEG）中具有躯体细胞的神经元，分别称为PI神经元，PL神经元和SEG神经元。 ，所有这些都投射到了后脑复合体Protophormia terraenovae中。免疫细胞化学结合通过心脏递回神经的回填显示，每对至少有两对PI和SEG神经元具有FMRFamide免疫反应活性。在PL神经元中观察到针对[Arg7 ]-corazonin，β-色素分散激素（β-PDH），胆囊收缩素8或FMRFamide的免疫反应性。在半球体PL的两到三个躯体中发现了[Arg7 ]-corazonin与β-PDH，[Arg7 ]-corazonin与胆囊收缩素8或β-PDH与FMRFamide的免疫反应共定位。根据其解剖和免疫细胞化学特征，将PI神经元分为两种类型，PL神经元分为六种类型，而SEG神经元则分为两种类型。脑后复合物中的纤维显示[Arg7 ]-corazonin，β-PDH，胆囊收缩素8和FMRFamide免疫反应性。在cardiac体的内在细胞中也检测到了胆囊收缩素8的免疫反应性。 FMRFamide免疫反应性曲张纤维密集地支配了文体。这些结果表明，PI，PL和SEG神经元从cardiac体或文体中释放[Arg7 ]-corazonin，β-PDH，胆囊收缩素8或FMRFamide样肽进入血淋巴，并且某些PL神经元可能同时释放几种神经肽。

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