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首页> 外文期刊>Cell and Tissue Research >Expression of VEGF-A, Flt-1, and Flk-1 in the arterial endothelial cells of the uterine broad ligament throughout the estrous cycle
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Expression of VEGF-A, Flt-1, and Flk-1 in the arterial endothelial cells of the uterine broad ligament throughout the estrous cycle

机译：在整个动情周期中，VEGF-A，Flt-1和Flk-1在子宫宽韧带的动脉内皮细胞中的表达

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摘要

The aim of the present study was to determine the immunoreactivity of vascular endothelial growth factor (VEGF-A) and its two receptors, viz., Flt-1 (fms-like tyrosine kinase) and Flk-1 (fetal liver kinase), on the surface of endothelial cells of the uterine artery and its branches and of the arcuate arteries in the area of the uterine broad ligament during various phases of the estrous cycle in the pig. We also investigated their expression to determine whether this was phase-related. The highest immunoreactivity for VEGF-A was observed in the uterine artery and arcuate arteries at the early luteal phase and in the branches of the uterine artery during the follicular phase of the estrous cycle. The strongest immunostaining intensity of Flt-1 was found in the uterine artery and its branches at the follicular phase and in arcuate arteries at the mid-luteal phase, whereas Flk-1 immunostaining was at its highest in the uterine artery at the mid-luteal phase and in the branches of the uterine artery and arcuate arteries at the follicular phase. Additionally, VEGF-A expression was assessed by semi-quantitative Western blot analysis, which revealed significantly higher levels of VEGF-A protein during the early luteal and the follicular phase of the estrous cycle (P < 0.001). The phase-related differences in the immunoreactivity and expression of VEGF-A and VEGF receptors suggest that these factors are hormone-dependent during the estrous cycle in the pig.
机译：本研究的目的是确定血管内皮生长因子（VEGF-A）及其两个受体，即Flt-1（fms样酪氨酸激酶）和Flk-1（胎儿肝激酶）的免疫反应性。在猪发情周期的各个阶段，子宫宽韧带区域的子宫动脉及其分支的内皮细胞表面和弓形动脉的表面。我们还研究了它们的表达，以确定这是否与阶段有关。在发情周期的卵泡期，在黄体早期和子宫动脉的分支中，在子宫动脉和弓状动脉中观察到了最高的VEGF-A免疫反应性。 Flt-1的免疫染色强度最强的是在卵泡期的子宫动脉及其分支中以及在黄体中期的弓形动脉中，而Flk-1的免疫染色在黄体中部的子宫动脉中最高。在卵泡期，在子宫动脉和弓形动脉的分支中。此外，通过半定量蛋白质印迹分析评估了VEGF-A的表达，发现在黄体早期和发情周期的卵泡期，VEGF-A蛋白水平显着升高（P <0.001）。 VEGF-A和VEGF受体的免疫反应性和表达的相位相关差异表明，这些因素在猪的发情周期中是激素依赖性的。

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  • 来源
    《Cell and Tissue Research》 |2007年第2期|313-319|共7页
  • 作者

    A. Postek; A. Andronowska; M. Chrusciel; K. Jankowska;

  • 作者单位

    Department of Reproductive Histophysiology Division of Reproductive Endocrinology and Pathophysiology Institute of Animal Reproduction and Food Research of Polish Academy of Sciences Tuwima 10 10-747 Olsztyn Poland;

    Department of Reproductive Histophysiology Division of Reproductive Endocrinology and Pathophysiology Institute of Animal Reproduction and Food Research of Polish Academy of Sciences Tuwima 10 10-747 Olsztyn Poland;

    Department of Reproductive Histophysiology Division of Reproductive Endocrinology and Pathophysiology Institute of Animal Reproduction and Food Research of Polish Academy of Sciences Tuwima 10 10-747 Olsztyn Poland;

    Department of Reproductive Histophysiology Division of Reproductive Endocrinology and Pathophysiology Institute of Animal Reproduction and Food Research of Polish Academy of Sciences Tuwima 10 10-747 Olsztyn Poland;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Vascular endothelial growth factor-A; Fms-like tyrosine kinase; Fetal liver kinase-1; Estrous cycle; Pig;

    机译：血管内皮生长因子-A;Fms样酪氨酸激酶;胎儿肝激酶-1;雌激素循环;猪;

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