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Assembly of Human Umbilical Vein Endothelial Cells on Compliant Hydrogels

机译：在符合标准的水凝胶上组装人脐静脉内皮细胞

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摘要

Angiogenesis is the process by which endothelial cells grow and disassemble into functional blood vessels. In this study, we examine the fundamental processes that control the assembly of endothelial cells into networks in vitro. Network assembly is known to be influenced by matrix mechanics and chemical signals. However, the roles of substrate stiffness and chemical signals in network formation are unclear. In this study, human umbilical vein endothelial cells (HUVECs) were seeded onto RGD or GFOGER functionalized polyacrylamide gels of varying stiffness. Cells were either treated with bFGF, VEGF, or left untreated and observed over time. We found that cells form stable networks on soft gels (Young’s modulus 140 Pa) when untreated but that growth factors induce increased cell migration which leads to network instability. On stiffer substrates (Young’s modulus 2500 Pa) cells do not assemble into networks either with or without growth factors in any combination. Our results indicate that cells assemble to networks below a critical compliance, that a critical cell density is needed for network formation, and that growth factors can inhibit network formation through an increase in motility.
机译：血管生成是内皮细胞生长并分解成功能性血管的过程。在这项研究中，我们研究了在体外控制内皮细胞组装成网络的基本过程。已知网络组装受矩阵力学和化学信号的影响。但是，衬底硬度和化学信号在网络形成中的作用尚不清楚。在这项研究中，将人脐静脉内皮细胞（HUVEC）播种到具有不同刚度的RGD或GFOGER功能化聚丙烯酰胺凝胶上。用bFGF，VEGF处理细胞，或不对其进行处理并随时间推移进行观察。我们发现，未经处理的细胞会在软凝胶（杨氏模量140 Pa）上形成稳定的网络，但生长因子会诱导细胞迁移增加，从而导致网络不稳定。在较硬的基材（杨氏模量2500 Pa）上，无论有无生长因子，细胞都不会组装成网络。我们的结果表明，细胞在低于临界柔度的情况下组装到网络中，形成网络需要临界细胞密度，并且生长因子可以通过运动性增加来抑制网络形成。

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