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首页> 外文期刊>Cellular and Molecular Bioengineering >Lateral Mobility of E-Cadherin Enhances Rac1 Response in Epithelial Cells
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Lateral Mobility of E-Cadherin Enhances Rac1 Response in Epithelial Cells

机译：E-钙黏着蛋白的横向移动性增强上皮细胞中的Rac1反应。

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摘要

The fluidity of cellular membranes imparts lateral mobility of proteins across the cell surface. To understand the impact of lateral mobility on cell–cell communication, a protein consisting of the extracellular recognition domains of E-cadherin was associated with the surface of silica beads by either tethering to a bead-supported lipid bilayer or direct adsorption, resulting in laterally mobile and immobile presentations of this protein. These beads were then seeded onto the upper surface of MDCK cells. Functional engagement of these beads was compared by measurement of Rac1 recruitment around the bead. Lateral mobility enhanced recognition of E-cadherin, promoting cell response to the beads at lower per-area concentrations than their immobilized counterparts. A more complete understanding of how lateral mobility of membrane-associated proteins influences molecular recognition, and potentially other downstream responses, could provide new strategies for the design of materials and devices intended to capture the architecture of natural tissues.
机译：细胞膜的流动性赋予蛋白质跨细胞表面的横向迁移性。为了了解横向迁移对细胞间通讯的影响，一种由E-钙粘蛋白的细胞外识别域组成的蛋白质通过束缚至珠支持的脂质双层或直接吸附而与硅胶珠表面相关联，从而导致蛋白质的移动和固定展示。然后将这些珠粒接种到MDCK细胞的上表面。通过测量珠子周围Rac1募集，比较了这些珠子的功能参与。横向移动性增强了对E-钙黏着蛋白的识别，在比固定化的相应区域更低的单位面积浓度下促进了细胞对磁珠的反应。对膜相关蛋白的横向迁移性如何影响分子识别以及潜在的其他下游反应的更完整的了解，可以为旨在捕获天然组织结构的材料和装置的设计提供新的策略。

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