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中华皮肤科杂志

中华皮肤科杂志

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Chinese Journal of Dermatology
中华医学会主办。本刊辟有述评、专家论坛、论著、短篇论著、新技术与新方法、临床经验、中医中药、皮肤外科、病例报告、皮肤科教程、药物与临床等原创性论文栏目，是了解中国在皮肤性病学预防和治疗、临床和实验研究工作最新进展的窗口。内容丰富，报道及时，具有指导性和实用性。
  • 发文量：7927
  • 被引量：2718
  • H指数：3
  • 开始收录时间：1989,22(2)
  • CNKI综合因子：0.603
  • 维普期刊影响因子： 1.92
  • 万方期刊影响因子：0.98
  • 创刊时间：1953
  • 国内刊号/CN：32-1138/R
    国际刊号/ISSN：0412-4030
  • 发行周期：月刊
    邮发代号：28-30
  • 主管单位：中国科学技术协会
    主办单位：中华医学会
中华皮肤科杂志-联系信息
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  • 邮箱：pifukezazhi@aliyun.com
  • 地址：南京市玄武区蒋王庙街12号
  • 邮编：210042
中华皮肤科杂志-获奖情况
  • • 2002年获第三届中国科协优秀科技期刊二等奖
  • • 2002年获华东地区第三届优秀期刊奖，同年在首届“江苏期刊方阵”评选中被评为双十佳期刊。
  • • 2000年本刊获中华医学会85周年优秀期刊银奖。
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中华皮肤科杂志 >2021年第19期

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  • 摘要：目的 探讨梅毒螺旋体对人脑微血管内皮细胞(HBMEC)趋化因子配体(CXCL)表达的影响.方法 将培养的HBMEC分为4组,分别加入用细胞培养液稀释的有活性的梅毒螺旋体、灭活的梅毒螺旋体、脂多糖和细胞培养液(梅毒螺旋体组、灭活梅毒螺旋体组、脂多糖组和空白对照组)刺激6、12、24h,用荧光定量PCR和酶联免疫吸附试验分别检测细胞中CXCL6、CXCL8和CXCL10基因和蛋白表达水平.利用Transwell小室细胞迁移实验检测梅毒螺旋体刺激后HBMEC对人早幼粒细胞白血病细胞HL-60的趋化作用.结果 在各时间点,梅毒螺旋体组HBMEC中CXCL6、CXCL8和CXCL10的mRNA表达水平均显著高于空白对照组和灭活梅毒螺旋体组(P＜0.05),而灭活梅毒螺旋体组与空白对照组之间差异无统计学意义(均P＞ 0.05).和脂多糖组相比,梅毒螺旋体组的CXCL6和CXCL8 mRNA表达降低(P＜0.05),CXCL10差异无统计学意义(P＞0.05).在各时间点,梅毒螺旋体组HBMEC培养上清液中CXCL6和CXCL8含量均高于空白对照组和灭活梅毒螺旋体组(均P＜0.05),而后2组间差异无统计学意义(均P＞0.05).各时间点培养上清液中CXCL10含量在梅毒螺旋体组、灭活梅毒螺旋体组和空白对照组间差异无统计学意义(均P＞ 0.05);梅毒螺旋体组Transwell小室下室中迁移的HL-60细胞数量(A570)均高于另2组(均P＜0.05).结论 有活性的梅毒螺旋体可上调HBMEC中CXCL6、CXCL8和CXCL10基因表达水平,增加CXCL6和CXCL8的分泌,增强HBMEC对HL-60细胞的趋化作用,这可能在神经梅毒的发病中起一定作用.%Objective To evaluate the effects of Treponema pallidum (T.pallidum) on the expression of chemokine ligands (CXCL) in human brain microvascular endothelial cells (HBMECs).Methods HBMECs were randomly divided into 4 groups,which were treated with viable T.pallidum suspension (T.pallidum group),heat-inactivated T.pallidum suspension (inactivated T.pallidum group),200 μg/L lipopolysaccharide (LPS group) and cell culture medium (blank control group),respectively,for 6,12 and 24 hours.Fluorescence-based quantitative PCR and enzyme-linked immunosorbent assay (ELISA) were performed to determine the mRNA and protein expression of CXCL6,CXCL8 and CXCL10 in HBMECs in the above groups respectively.Transwell migration assay was conducted to evaluate the effects of T.pallidum-stimulated HBMECs on the chemotaxis of human promyelocytic HL-60 leukemia cells (HL-60 cells).Results At 6,12 and 24 hours,the T.pallidum group showed significantly higher mRNA expression of CXCL6,CXCL8 and CXCL10 in HBMECs compared with the blank control group and inactivated T.pallidum group (all P ＜ 0.05),while there were no significant differences between the blank control group and inactivated T.pallidum group (all P ＞ 0.05).Compared with the LPS group,the T.pallidum group showed significantly decreased mRNA expression of CXCL6 and CXCL8 (P ＜ 0.05),but similar mRNA expression of CXCL10(P ＞ 0.05)at 6,12 and 24 hours.At these time points,the levels of CXCL6 and CXCL8 in the culture supernatant of HBMECs were significantly higher in the T.pallidum group than in the blank control group and the inactivated T.pallidum group (all P ＜ 0.05),but no significant differences were observed between the blank control group and the inactivated T.pallidum group (both P ＞ 0.05).Moreover,there were no significant differences in the level of CXCL10 in the culture supernatant of HBMECs among the T.pallidum group,the inactivated T.pallidum group and the blank control group (all P ＞ 0.05).The number of migratory HL-60 cells in the lower Transwell chambers was significantly higher in the T.pallidum group than in the inactivated T.pallidum group and the blank control group (both P ＜ 0.05).Conclusion Viable T.pallidum can up-regulate the gene expression of CXCL6,CXCL8 and CXCL10 in HBMECs,promote the secretion of CXCL6 and CXCL8,and enhance the chemotactic effect of HBMECs on HL-60 cells,which may play a certain role in the occurrence of neurosyphilis.
  • 摘要：目的 分离培养广东地区梅毒螺旋体(Tp)临床株(GD1)并探讨其与Nichols标准株非同义单核苷酸多态性(nsSNPs)的差异.方法 从广东地区1例硬下疳患者皮损中分离出GD1,在兔睾丸中连续传代培养.暗视野显微镜、TP0548基因PCR、测序和基因分型多重验证传代情况.泛影葡胺梯度离心法分离兔组织并浓缩GD1.测序验证TP0443和TP0584基因nsSNPs位点.结果 本研究分离培养出的GD1株为TP0548f亚型.与美国Nichols株比较,GD1株存在nsSNPs突变,位于TP0443的突变导致编码的第120位氨基酸由苏氨酸变为丙氨酸,TP0584的突变导致编码的第314位氨基酸由丙氨酸变为苏氨酸.结论 首次成功地从广东地区梅毒患者皮损中分离培养出GD1株Tp,并从病原学水平证实GD1存在nsSNPs突变.%Objective To isolate and culture a clinical strain (GDI) of Treponema pallidum (Tp) in Guangdong province,and to investigate the difference in nonsynonymous single nucleotide polymorphisms (nsSNPs) between the GD1 strain and Tp Nichols strain.Methods The GD1 strain was isolated from the hard chancre in a patient with primary syphilis in Guangdong province,and continuously subcultured in the testes of New Zealand white rabbit.The serial subcultivation of GD1 was multi-verified by dark-field microscopy,polymerase chain reaction (PCR) for TP0548 gene,DNA sequencing and genotyping.Meglumine diatrizoate density gradient centrifugation was performed to isolate rabbit tissues and concentrate GD1,and DNA sequencing was used to verify the nsSNPs in the TP0443 and TP0584 genes.Results The GD1 strain was successfully isolated from the lesions of the patient with syphilis,and classified as a subtype f of TP0548.Compared with the American Tp (Nichols strain),there were nsSNP mutations in the GD1 strain.One mutation was located in the TP0443 gene,leading to the the substitution of threonine by alanine at amino acid position 120,and another one was located in the TP0584 gene,which caused a change from alanine to threonine at amino acid position 314.Conclusion The GD1 strain was successfully isolated firstly from the lesions in a patient with syphilis in Guangdong province,and nsSNP mutations were confirmed in the GD 1 strain on the etiology.
  • 摘要：目的 评估Bumet研究所自制的IgA快速检测试剂(POC)对早期梅毒的诊断性能.方法 利用性病中心参比实验室库存的455份血清标本进行梅毒IgA-POC应用性评估,标本根据梅毒螺旋体血球凝集试验(TPHA)、快速血浆反应素环状卡片试验(RPR)及IgM酶联免疫吸附试验(IgM-ELISA)结果分为未感染组、既往感染组和早期现症组.对3组标本进行IgA-POC盲样检测,评估其对现症梅毒的诊断能力.结果 IgA-POC对早期现症组的诊断灵敏度为92.6％(147/163),对既往感染组的排除诊断特异性为72.22％(104/144);对未感染的排除诊断特异性为97.97％(145/148),总特异性为85.27％,达到了WHO对梅毒快速诊断试剂的最低要求.IgA-POC对早期现症梅毒诊断灵敏度高于IgM-ELISA (59.51％)(Z=6.88,P＜0.05),但其对既往感染的排除诊断特异性低于IgM-ELISA(98.61％) (Z=6.18,P＜0.05),两种方法对未感染的排除诊断特异性差异无统计学意义(Z=1.16,P=0.25).结论 IgA-POC对早期现症梅毒的发现能力较IgM-ELISA强,可以应用于早期现症梅毒的筛查.%Objective To evaluate the application of Burnet Institute-made prototype IgA rapid test,a kind of point-of-care (POC) testing,in the diagnosis of early syphilis.Methods Totally,455 stored serum samples in the Reference Laboratory of Sexually Transmitted Disease,the Institute of Dermatology were used to evaluate the application of the prototype IgA rapid test (IgA-POC) in the diagnosis of early syphilis.According to resluts of Treponema pallidum hemagglutination assay (TPHA),rapid plasma reagin card test (RPR),and enzyme-linked immunosorbent assay for IgM antibodies (IgM-ELISA),these stored samples were divided into 3 groups:uninfected group,previously infected group and early active syphilis group.IgA-POC test was performed in the 3 groups to evaluate its diagnostic performance for active syphilis,and researchers were blind to the group information.Results The prototype IgA-POC test had a sensitivity of 92.6％ (147/163) for the early active syphilis group,a specificity of 72.22％ (104/144) for the previously infected group,and a specificity of 97.97％ (145/148) for the uninfected group.The total specificity of the prototype IgA-POC test was 85.27％,which met the minimum requirement of WHO for the POC test.The prototype IgA-POC test showed a significantly higher sensitivity for the diagnosis of early active syphilis compared with the IgM-ELISA (59.51％,Z =6.88,P ＜ 0.05),but a significantly lower specificity for the diagnosis of previous syphilis infection compared with the IgM-ELISA (98.61％,Z =6.18,P ＜ 0.05).Moreover,no significant difference in the specificity for the diagnosis of non-infection was observed between the prototype IgA-POC test and IgM-ELISA (Z =1.16,P =0.25).Conclusion The prototype IgA-POC test has better capacity for the diagnosis of early active syphilis compared with the IgM-ELISA,so it can be applied to the screening of early active syphilis.
  • 摘要：目的 探讨梅毒螺旋体(Tp)体外诱导巨噬细胞分泌的外泌体特征及其对人脐静脉内皮细胞(HUVEC)增殖水平的影响.方法 自雄兔睾丸分离Tp.将人单核巨噬细胞(THP-1)诱导为巨噬细胞后,分为实验组(Tp刺激)和对照组(不用Tp刺激),12h后继续培养48 h,收集巨噬细胞外泌体悬液,利用差速离心法和膜亲和试剂盒提取外泌体,运用透射电镜、Western印迹鉴定外泌体,Nanoparticle tracking analysis (NTA)粒径分析仪定量分析外泌体.将HUVEC分为实验组、对照组和外泌体洗脱液组,分别用外泌体悬液(4.5×108颗粒,实验组和对照组)、外泌体洗脱液刺激培养一定时间后,激光共聚焦扫描显微镜观察HUVEC对外泌体的吞噬,CCK8法检测HUVEC增殖活性.结果 透射电镜下的外泌体呈杯托样,为直径30～100 nm的微小囊泡.Western印迹显示,外泌体丰富表达膜蛋白CD63、CD9和CD81.相同实验条件下,NTA测定显示,实验组与对照组外泌体粒径(u=1.90,P＞ 0.05)、浓度(z=-1.604,P=0.109)差异均无统计学意义.两组外泌体分别与HUVEC共培养5h后,激光共聚焦显微镜下见胞内散在分布被吞噬的绿色荧光外泌体.实验组、对照组外泌体分别作用于HUVEC 12 h后细胞增殖水平均高于外泌体洗脱液组,差异均有统计学意义(P＜0.05);在24 h和48 h时对照组细胞增殖水平仍高于外泌体洗脱液组,在48 h达到峰值(P＜0.05),实验组细胞增殖水平趋于正常;在72 h时实验组、对照组细胞增殖水平与外泌体洗脱液组差异无统计学意义(P＞0.05).结论 THP-1来源的巨噬细胞分泌的外泌体在48 h内能显著提高HUVEC细胞增殖水平,在48 h达到峰值.在Tp刺激下,THP-1来源的巨噬细胞分泌的外泌体在形态、大小、浓度上与无Tp刺激巨噬细胞分泌的外泌体相似,但仅在短时间(12h)内提高HUVEC增殖水平.%Objective To investigate characteristics of Treponema pallidum (Tp)-induced macrophage-derived exosomes and its effect on the proliferation of human umbilical vein endothelial cells (HUVEC).Methods Tp strains were collected from the testis of male rabbit,which were infected with Tp (Nichols strain).The human mononuclear macrophages (THP-1) were induced into macrophages by incubation with propylene glycol methyl ether acetate (PMA),and then the macrophages were divided into 2 groups:experimental group incubated with Tp for 12 hours followed by 48-hour normal culture,and control group receiving normal culture.After the treatment,exosome suspensions were collected,and exosomes were extracted by differential centrifugation and exoEasy Maxi Kit.Transmission electron microscopy and Western blot analyses were performed to identify the exosomes,and nanoparticle tracking analysis (NTA) was conducted to measure the diameters and concentrations of exosomes.In vitro cultured HUVECs were divided into 3 groups,which were cultured with the 10 μl of suspensions containing exosomes derived from Tp-stimulated macrophages at a concentration of 4.5 × 108/ml (experimental group),10 μl of suspensions containing exosomes derived from untreated macrophages at a concentration of 4.5 × 108/ml (control group),and 10 μl of exosome eluents (exosome eluent group),respectively.After the treatment,confocal laser scanning microscopy was performed to observe the phagocytosis of exosomes of HUVECs,and cell counting kit-8 to evaluate the proliferative activity of HUVECs.Results The exosomes were saucer-like microvesicles with diameters of 30-100 nm under the transmission electron microscope.Western blot analyses showed that membrane proteins CD63,CD9 and CD81 were abundantly expressed by exosomes.Under the same conditions,NTA revealed that there were no significant differences in the particle diameter (u =1.90,P ＞ 0.05) and concentration of exosomes (Z =-1.604,P =0.109) between the experimental group and the control group.After co-culture with HUVECs for 5 hours,confocal laser scanning microscopy showed scatteredly distributed exosomes with green fluorescence in the HUVECs in the experimental group and control group.After 12-hour co-culture with the exosome suspensions,the proliferative activity of HUVECs was significantly higher in the experimental group and the control group than in the exosome eluent group (both P ＜ 0.05).After 24-and 48-hour treatment with exosome suspensions,the proliferative activity of HUVECs in the control group was still significantly increased compared with that in the exosome eluent group,and peaked at 48 hours (all P ＜ 0.05).Moreover,there were no significant differences in the proliferative activity of HUVECs between the experimental group and exosome eluent group at 24 and 48 hours (both P ＞ 0.05).At 72 hours,no significant differences in the proliferative activity of HUVECs were observed among the experimental group,the control group and the exosome eluent group (all P ＞ 0.05).Conclusions The exosomes secreted by THP-1 cells-derived macrophages evidently increased the proliferative activity of HUVECs within 48 hours,which peaked at 48 hours.After the stimulation with Tp,the exosomes secreted by THP-1 cells-derived macrophages were similar to those without Tp stimulation in morphology,size and concentration,and only increased the proliferative activity of HUVECs within 12 hours.
  • 摘要：A 55-year-old male patient presented with plaques on the face for more than 20 years,and no immunodeficiency diseases were diagnosed.Skin examination showed large areas of pink plaques on the nose,bilateral cheeks and upper oral lips with slight desquamation,verrucous hyperplasia on the dorsal area of the nose,and a bean-sized verrucous protuberance on the tip of the nose.Histopathological examination of the skin lesions revealed pseudoepitheliomatous hyperplasia in the epidermis and hyphae-like structures in the stratum corneum.Moreover,there was diffuse infiltration of inflammatory cells in the dermis,which mainly included neutrophils,lymphocytes,histiocytes and multinucleated giant cells.Periodic acid-Schiff (PAS)-positive spore-like structures were observed in the multinucleated giant cells.Culture of the lesional tissues on Sabouraud dextrose agar (SDA) medium showed grey-brown villous colonies.Microculture on the potato dextrose agar (PDA) medium yielded dark septate hyphae and pycnidia filled with a large number of spores.Microsphaeropsis arundinis was identified by fungal molecular biological techniques.The patient was diagnosed with cutaneous phaeohyphomycosis caused by Microsphaeropsis arundinis.The patient was treated with CO2 laser for the removal of verrucous protuberance on the tip of the nose,and oral itraconazole capsules at a dose of 200 mg twice a day.After 3-month treatment,the skin lesions subsided and the drug was withdrew.During 6-month follow-up,no relapse occurred.%患者男,55岁,面部斑块20余年,未发现合并免疫缺陷疾病.皮肤科检查:鼻部、双面颊、口唇上方大片淡红色斑块,少量脱屑,鼻背部疣状增生,鼻尖1个黄豆大小的疣状突起物.皮损组织病理检查:表皮假上皮瘤样增生,角质层中可见菌丝样结构.真皮内弥漫炎症细胞浸润,主要有中性粒细胞、淋巴细胞、组织细胞以及多核巨细胞.在多核巨细胞内见孢子样结构,过碘酸-雪夫染色阳性.皮损组织沙氏葡萄糖琼脂培养基培养出灰褐色绒毛状菌落.玻片小培养(马铃薯葡萄糖琼脂培养基)见内含大量孢子的分生孢子器及分隔分支的暗色菌丝.真菌分子生物学鉴定为Microsphaeropsisarundinis.诊断为Microsphaeropsis arundinis所致皮肤暗色丝孢霉病.治疗:CO2激光去除鼻尖部位疣状突起,口服伊曲康唑胶囊200 mg,每天2次,3个月后皮损消退停药,随访6个月无复发.
  • 摘要：Objective To investigate epidemiological features of genital herpes in China from 2008 to 2017,and to provide scientific evidences for developing control strategies.Methods A descriptive epidemiological study was conducted to analyze personal,spatial and temporal distribution of genital herpes cases reported from 105 sexually transmitted disease (STD) surveillance sites between 2008 and 2017.Results The reported incidence of genital herpes decreased from 8.30 per 100 000 in 2008 to 6.14 per 100 000 in 2017,with an average annual decline rate of 3.29％.There was marked variation in the reported incidence of genital herpes among different STD surveillance sites,with a range from ＜ 1 per 100 000 to 113.51 per 100 000.The reported incidence was relatively high in Guangdong,Guangxi,Hainan,Zhejiang,Fujian,Chongqing and Hubei,but low in north China and central China.In addition,no cases were reported at a few sites in rural areas.The reported annual incidence was higher in females than in males in 2016,but higher in males than in females in the other years.The ratio of male to female cases decreased from 1.77 ∶ 1 in 2008 to 1.03:1 in 2017.Among all the age groups,the sexually active population aged 20-39 years showed high reported incidence of genital herpes,with the highest reported incidence observed in the group aged 25-34 years (range,17.84/100 000-26.89/100 000).Among all kinds of medical institutions,general hospitals had the largest number of reported cases of genital herpes (range,58.13％-76.52％),followed by specialized dermatovenereology hospitals,gynecology hospitals and maternal and child health (MCH) hospitals.Conclusions Although the overall reported incidence of genital herpes shows a downward trend,the reported incidence is high in Guangdong,Guangxi,Hainan,Zhejiang,Fujian,Chongqing and Hubei.Therefore,different prevention strategies are needed to be developed according to the epidemiological features.%目的 了解2008-2017年全国生殖器疱疹流行特征,为制定防控对策提供依据.方法 应用描述性流行病学方法对2008-2017年全国105个国家性病监测点报告的生殖器疱疹病例资料进行“三间分布”分析.结果 生殖器疱疹报告发病率从2008年的8.30/10万下降到2017年的6.14/10万,年均下降3.29％.不同监测点报告发病率差异较大,最高可达113.51/10万,最低＜1/10万.高发地区主要为广东、广西、海南、浙江、福建、重庆和湖北,报告发病率较低的地区主要为华北和中部地区,少数农村监测点无病例报告.2016年女性报告发病率略高于男性,其他年份男性均高于女性,男女性别比由2008年的1.77∶1下降至2017年的1.03∶1.生殖器疱疹高发年龄段为20～ 39岁的性活跃人群,以25～34岁年龄组报告发病率最高(17.84/10万～26.89/10万).综合医院报告病例数最多,占58.13％～ 76.52％,其次为皮肤性病专科医院、妇科医院和妇幼保健院.结论 虽然中国生殖器疱疹报告发病率整体呈下降趋势,但在广东、广西、海南、浙江、福建、重庆和湖北发病率仍较高,应根据各地疾病流行特点有针对性地采取不同的防治措施.
  • 摘要：Objective To investigate the spatial distribution characteristics and patterns of congenital syphilis in China,and to make suggestions and recommendations for precise prevention of motherto-child transmission of syphilis.Methods The geographic information system software ArcGIS 10.0 was used to conduct exploratory spatial data analysis on the basis of congenital syphilis cases reported from 31 provinces,autonomous regions and municipalities directly under the central government in 2010 and 2015.Results In 2015,a total of 7 251 congenital syphilis cases were reported,which decreased by 40.00％ compared with the case number in 2010 (12 084 cases).The global spatial autocorrelation analysis revealed a positive spatial autocorrelation in the number of reported cases at the county level in China between 2010 and 2015,with Global Moran's I indices of 0.46 and 0.37,respectively.The degree of spatial clustering of congenital syphilis was lower in 2015 than in 2010.The local spatial autocorrelation analysis was further performed to output the hot-spot mappings of congenital syphilis at the county level in 2010 and 2015.In 2010,207 hot-spot counties with a high number of congenital syphilis cases (＞ 12 cases) were identified,and these counties were mainly distributed in 3 core areas including southwest China (Chongqing,eastern Sichuan and northern Guizhou),eastern China (southern Jiangsu,Zhejiang and northern Fujian),and southern and central China (Guangxi,Guangdong and mid-east Hunan).In 2015,72 hot-spot counties with a high number of congenital syphilis cases (＞ 12 cases)were identified,and mainly distributed in 5 core areas including northwest China (western Xinjiang),mid-east Qinghai,southwest and central China (Sichuan,Chongqing,Guizhou and Hunan),eastern China (Jiangsu,Anhui),and southern China and eastern China (Guangdong and Fujian).Conclusions In Zhejiang,Shanghai,Guangdong,Guangxi,Sichuan and Chongqing,the number of hot-spot counties with a high reported congenital syphilis cases was significantly lower in 2015 than in 2010,suggesting that the prevention of mother-to-child transmission of syphilis had achieved a primary success.At present,hot-spots counties with a high number of congenital syphilis cases are mainly distributed in western Xinjiang,mid-east Qinghai,Sichuan,Chongqing,Guizhou and so on,Therefore,the prevention of mother-to-child transmission of syphilis should be strengthened in these areas.%目的 分析我国胎传梅毒空间分布特征,为制定精准的梅毒母婴传播预防策略提供依据.方法 收集2010年、2015年全国31个省、自治区、直辖市报告的胎传梅毒病例数据,使用地理信息系统ArcGIS 10.0软件进行胎传梅毒疫情的探索性空间分析.结果 2015年全国共报告7 251例胎传梅毒,较2010年(12084例)下降了40.00％.全局空间自相关分析结果显示,2010年和2015年全国县区级胎传梅毒报告病例数全局Moran I指数分别为0.46和0.37,均呈显著的空间正相关,2015年胎传梅毒疫情的空间聚集性低于2010年.通过局部空间自相关分析输出我国县区级胎传梅毒疫情分布热点地图:2010年呈现3个核心区域,主要分布于西南部分地区,包括重庆、四川东部和贵州北部;华东部分地区,包括江苏南部、浙江和福建北部;华南和华中部分地区,包括广西、广东和湖南中东部,共发现207个热点县区(病例数＞12);2015年呈现5个核心区域,主要分布于西北地区的新疆西部;青海中东部;西南与华中部分地区,包括四川、重庆、贵州和湖南;华东部分地区,包括江苏与安徽;华南与华东部分地区,包括广东与福建,共发现72个热点县区(病例数＞ 12).结论 2015年浙江、上海、广东、广西、四川、重庆等地的胎传梅毒疫情热点县区数量明显少于2010年,预防梅毒母婴传播工作初见成效.现阶段我国胎传梅毒疫情热点县区主要分布于新疆西部、青海中东部、四川、重庆、贵州等地,今后应重点加强这些地区的梅毒母婴传播预防工作.
  • 摘要：目的 筛选鉴定并评价梅毒螺旋体(Tp)体内诱生抗原Tp0462的临床血清学诊断价值.方法 提取Tp Nichols株全基因组,PCR扩增Tp0462,克隆构建重组质粒pET30a(+)-Tp0462,转化至E.coli Rosetta (DE3)菌,大量诱导表达重组蛋白Tp0462并纯化、鉴定.18只新西兰兔随机平均分为活Tp接种组、紫外线照射灭活Tp接种组和未接种对照组,接种后不同时间点抽血分离血清,用ELISA鉴定Tp0462蛋白的体内诱生性特点.ELISA法(Tp0462-ELISA)、梅毒螺旋体明胶凝集试验(TPPA)、梅毒初筛ELISA试验及快速血浆反应素环状卡片试验(RPR)试验检测336份临床梅毒患者血清样本,初步评价该蛋白的诊断价值.结果 重组质粒Tp0462-pET30a(+)在E.coli Rosetta (DE3)中最适的表达条件为0.5 mmol/L IPTG,20℃,180rpm摇床诱导培养4h.活Tp接种组血清Tp0462特异性抗体水平从第2周起呈急剧上升趋势,至5周后趋于平稳,而灭活Tp接种组及未接种对照组血清Tp0462特异性抗体水平一直处于低水平.活Tp接种组Tp0462特异性抗体水平显著高于灭活Tp接种组及未处理组(P＜0.05),而灭活Tp接种组与未处理组相比,差异无统计学意义(P=0.256).活Tp接种组、灭活Tp接种组Tp92抗体水平显著高于未处理组(P＜0.05),而活Tp接种组与灭活Tp接种组差异无统计学意义(P=0.127).与TPPA相比,Tp0462-ELISA的灵敏度和特异度分别为91.7％和98.8％,符合率为95.2％,曲线下面积为0.997.Tp0462-ELISA与梅毒初筛ELISA试验的符合率为92.3％,Kappa值为0.846;与RPR试剂盒的符合率为83.9％,Kappa值为0.293.结论 Tp0462在梅毒血清学诊断中具有较高的灵敏度和特异度,可以作为潜在的梅毒诊断候选蛋白.%Objective To screen and identify the in vivo-induced antigen Tp0462 of Treponema pallidum (Tp),and to evaluate its value for clinical serological diagnosis of syphilis.Methods Genomewide DNA was extracted from the Tp Nichols strain,and polymerase chain reaction (PCR) was performed to amplify the Tp0462 gene.A recombinant plasmid pET30a (+)-Tp0462 was constructed and transfected into the Escherichia coli (E.coli) Rosetta (DE3) strain.Recombinant protein Tp0462 was abundantly expressed,purified and identified.A total of 18 New Zealand rabbits were randomly and equally divided into 3 groups:viable Tp-incubating group incubated with viable Tp in the testes,inactived Tp-incubating group incubated with ultraviolet irradiation-killed Tp in the testes,and control group receiving no treatment.After incubation,blood samples were collected at different time points,and the sera were isolated for identification of characteristics of in vivo-induced antigen Tp0462.Enzyme-linked immunosorbent assay for Tp0462 (Tp0462-ELISA),Treponema pallidum particle agglutination assay (TPPA),preliminary syphilis screening ELISA and rapid plasma reagin (RPR) card test were applied in 336 clinical serum samples from patients with syphilis,so as to preliminarily evaluate the value of Tp0462 for the diagnosis of syphilis.Results The optimum conditions for expression of the recombinant plasmid Tp0462-pET30a(+) in E.coli Rosetta (DE3) strains were the treatment with 0.5 mmol/L isopropyl thiogalactoside (IPTG) on a shaker at 180 rpm for 4 hours.In the viable Tp-incubating group,the serum level of specific anti-Tp0462 antibody sharply increased from week 2,and went steady after week 5.However,the specific anti-Tp0462 antibody maintained a low level in the inactived Tp-incubating group and the control group.The viable Tp-incubating group showed a significantly higher level of specific anti-Tp0462 antibody compared with the inactived Tp-incubating group and the control group (both P ＜ 0.05),while no significant difference was observed between the inactived Tp-incubating group and the control group (P =0.256).The level of anti-Tp92 antibody was significantly higher in the viable Tp-incubating group and the inactived Tp-incubating group than in the control group (P ＜ 0.05),while there was no significant difference between the viable Tp-incubating group and the inactived Tp-incubating group (P =0.127).Compared with TPPA,the sensitivity,specificity,consistency rate and area under the curve (AUC) of Tp0462-ELISA for the diagnosis of syphilis were 91.7％,98.8％,95.2％ and 0.997 respectively.Tp0462-ELISA was consistent to preliminary syphilis screening ELISA and RPR with a Kappa coefficient of 0.846 and 0.293,respectively.Conclusion Tp0462-ELISA has shown evidently higher sensitivity and specificity in the serodiagnosis of syphilis,and Tp0462 can serve as promising antigens for the diagnosis of syphilis.
  • 摘要：目的 评价群组看病及同伴教育对痤疮患者遵医行为的影响,探讨痤疮患者的有效健康教育方式.方法 选取2016年12月在中国医学科学院皮肤病医院就诊的80例痤疮患者作为研究对象,按单纯随机分组方法分为干预组和对照组,每组40例.干预组给予常规健康教育的同时接受群组看病及同伴教育,对照组给予常规健康教育.在干预后1、2、4周进行遵医行为调查,比较两组的遵医行为.结果 因拒访和无应答原因脱落18例,最终干预组32例、对照组30例纳入分析.在干预1、2、4周后,干预组遵医行为总分分别为83.6±9.3、85.9±9.1、91.2±8.4,对照组分别为77.1±7.3、77.1±8.6、79.1±10.2,干预组遵医行为的提高显著优于对照组(P组间＜0.05),随着干预时间的增加显著性地提高(P时间＜0.05),时间和分组之间有交互作用(P交互＜0.05).结论 群组看病及同伴教育的健康教育模式能提高痤疮患者遵医行为.%Objective To evaluate the effects of group medical visits and peer education on the compliance behaviors of patients with acne vulgaris,and to explore the effective health education approaches for patients with acne vulgaris.Methods A total of 80 patients with acne vulgaris were enrolled from Hospital of Dermatology,Chinese Academy of Medical Sciences during December 2016.They were randomly and equally divided into 2 groups:intervention group receiving conventional health education combined with group medical visits and peer education,and control group receiving conventional health education alone.At week 1,2 and 4 after the intervention,the compliance behaviors were compared between the 2 groups.Results Totally,18 patients were lost to the follow-up due to refusals and non-response,and 32 patients in the intervention group and 30 patients in the control group finally completed the study.At week 1,2 and 4 after the intervention,the scores of compliance behaviors were significantly higher in the intervention group (83.6 ± 9.3,85.9 ± 9.1,91.2 ± 8.4 respectively) than in the control group (77.1 ± 7.3,77.1 ± 8.6,79.1 ± 10.2 respectively;all P ＜ 0.05).Moreover,the scores of compliance behaviors significantly increased over time (P ＜ 0.05),and there was a significant interaction effect between the intervention methods and treatment duration (P ＜ 0.05).Conclusion Health education approaches including group medical visits and peer education can improve the compliance behaviors of patients with acne vulgaris.
  • 摘要：A female infant presented with skin defects and blisters for 2 hours after birth.Physical examination showed asymmetric skin defects on both lower extremities and left wrist,a thin-walled blister on the dorsal side of the right hand,and partial loss of the oral mucosa.No other abnormal signs were found.Genetic testing showed a heterozygous pathogenic mutation c.481C ＞ T (p.Gln161*) in exon 4 of the COL7A1 (NM-000094) gene and a heterozygous pathogenic mutation c.1837C ＞ T (p.Arg613*) in exon 14 of the COL7A1 (NM-000094) gene,which were also identified in the patient's father and mother respectively.The infant was diagnosed with congenital skin defects.The patient received protective isolation,focal washing with 0.9％ sodium chloride physiological solution,topical epidermal growth factor and comprehensive treatment for infection prevention.After 6-day treatment,the patient was discharged with dry and non-exudative skin lesions.This case prompted that abnormal heterozygosis mutation at C.481 and C.1837 sites on the COL7A1 (NM-000094) gene could form compound heterozygote,acting as pathogenic mutation.%患儿女,生后2h,因皮肤缺损并水疱2h就诊.体检:双下肢及左手腕皮肤成不对称缺损,右手手背可见一薄壁水疱,口腔黏膜部分缺失,余体检未见异常.基因检测结果:COL7A1(NM-000094) exon4:c.48 1c＞T;P.(Gln161*)杂合,致病突变;COL7A1 (NM-000094) exon 14:c.1837C＞T;P.(Arg613*)杂合,致病突变.患儿父母基因检测各有一致病突变.诊断:先天性皮肤发育不全.给予患儿保护性隔离,生理氯化钠溶液局部冲洗,并外用表皮生长因子、防治感染综合治疗,治疗6d出院,出院时皮损干燥无渗出.该病例提示,COL7A1 (NM-000094)基因C.481位点和C.1837位点的杂合异常组成复合杂合子,为致病突变.
  • 摘要：目的 评价308 nm准分子激光与高能紫外线治疗进展期局限型白癜风的疗效与安全性,皮肤共聚焦显微镜观察治疗前后皮损区改变.方法 进展期局限型白癜风患者60例203片皮损,每例均有相同解剖部位皮损≥3片,其中1片作为空白对照,另外≥2片皮损随机接受308 nm准分子激光(激光组)与高能紫外线(紫外线组)治疗,每周2次,共治疗25次.结果 48例患者169片皮损完成试验.激光组显效率[66.15％ (43/65)]高于紫外线组[44.64％(25/56)],x2=8.28,P＜0.01.白癜风活动度2分组显效率[67.69％(44/65)]高于3分组[44.64％(25/56)],x2=6.80,P＜0.01.随访3个月,未见复发.皮肤共聚焦显微镜下发现,部分皮损边界线内外真表皮交界处炎症细胞增多,治疗后,炎症细胞数量显著减少,部分皮损炎症细胞数量完全恢复正常.结论 308 nm准分子激光与高能紫外线治疗进展期局限型白癜风疗效显著,308 nm准分子激光见效更快,疗效更好.%Objective To investigate the efficacy and safety of 308-nm excimer laser compared to high-intensity ultraviolet radiation for the treatment of active localized vitiligo,and to observe changes in skin lesions before and after the treatment by confocal laser scanning microscopy.Methods Sixty patients with 203 skin lesions of active localized vitiligo and were enrolled into this study,and the vitiligo disease activity (VIDA) score of these patients ranged from 2 to 3.We selected more than 3 skin lesions from a same anatomical site of each patient,one of lesions served as a control and the other skin lesions (≥ 2) were randomly treated with 308-nm excimer laser (laser group) or high-intensity ultraviolet radiation (ultraviolet group).The treatment was conducted twice a week for 25 sessions,and a 3-month follow-up was performed.Results A total of 48 patients with 169 skin lesions completed the trial.The marked response rate was significantly higher in the laser group [66.15％ (43/65)] than in the ultraviolet group [44.64％ (25/56),x2 =8.28,P ＜ 0.01].The patients with a VIDA score of 2 showed a significantly higher marked response rate [67.69％(44/65)] compared with those with a VIDA score of 3 [44.64％(25/56),x2 =6.80,P ＜ 0.01].During the 3-month follow-up,no relapse was observed.Confocal laser scanning microscopy showed that the number of inflammatory cells increased at the dermal-epidermal junction of the intra-and extra-marginal lesional skin.After treatment,the number of inflammatory cells markedly decreased and returned to normal level in lesions.Conclusion Both 308-nm excimer laser and high-intensity ultraviolet radiation are effective in the treatment of active localized vitiligo,but the 308-nm excimer laser shows a more rapid onset of action and a better therapeutic effect.
  • 摘要：Objective To evaluate the regulatory effects of lycopene on the key signaling receptors in human cutaneous squamous cell carcinoma cell line COLO16.Methods Cultured COLO16 cells were divided into 6 groups to be treated with lycopene at different concentrations of 0,5,10,15,20,and 25 μmol/L,respectively,for 24 hours (control group and 5,10,15,20,25 μmol/L lycopene groups),followed by estimation of the cell viability by lactate dehydrogenase (LDH) assay.Lycopene at a safe concentration was selected based on the LDH assay,and used for the determination of expression of signaling receptors,and Western blot analysis was performed to measure the expression of key signaling receptor proteins,including epidermal growth factor receptor (EGFR),glucocorticoid receptor (GR),retinoic acid receptor-alpha (RAR-α),retinoid X receptor-alpha (RXR-α),androgen receptor (AR) and progesterone receptor (PR).Statistical analysis was carried out by one-way analysis of variance (ANOVA),Tukey multiple comparison teat and Brown-Forsythe test with the SPSS software.Results After 24-hour treatment with lycopene at different concentrations,there were significant differences in the rate of cell death among these groups (F =13.116,P ＜ 0.05),and the rate of cell death in the 25 μmol/L lycopene group significantly differed from that in the control group (P ＜ 0.05).Therefor,lycopene at concentrations of 5,10 and 20 μmol/L were selected to treat COLO16 and HaCaT cells as well as human epidermal keratinocyte (HEK) for 24 hours in the following experiment.The treatment with lycopene significantly decreased the phosphorylation level of EGFR (P ＜ 0.05),but significantly increased the expression of GR protein (P ＜ 0.05),and showed no significant effects on the protein expression of RAR-α,RXR-α,AR,and PR in COLO16 cells.After 24-hour treatment with lycopene at concentrations of 5,10 and 20 μmol/L,there were no significant changes in the phosphorylation level of EGFR protein or the expression of GR protein in HaCaT cells and HEK (all P ＞ 0.05) compared with those without lycopene treatment.Conclusion Lycopene can decrease the viability of COLO16 cells,inhibit the activation of EGFR protein,and up-regulate the expression of GR,and these effects may be specific for tumor cells.%目的 探讨番茄红素对人皮肤鳞状细胞癌细胞系COLO16细胞关键信号受体调控的影响.方法 COLO16细胞分为0(对照组)、5、10、15、20、25 μmol/L番茄红素6个处理组,处理24 h后进行LDH实验确定对细胞活力的影响.根据LDH分泌量确定细胞死亡水平,选取番茄红素对细胞作用的安全浓度信号受体表达量实验,蛋白免疫印迹检测关键信号受体蛋白表达水平.使用SPSS软件进行单因素方差分析,采用Tukey Multiple Comparison和Brown-Forsythe (B)检验方法.结果 5、10、15、20、25 μmol/L番茄红素处理COLO16细胞24 h后,25 μmol/L组细胞死亡率和对照组比差异有统计学意义(F=13.116,P＜0.05),选取5、10、20μmol/L三个浓度番茄红素处理COLO16细胞24 h后,表皮生长因子受体蛋白磷酸化水平降低(P＜0.05),糖皮质激素受体蛋白表达水平升高(P＜0.05),而维A酸受体、雄激素受体、孕激素受体蛋白表达水平变化不显著.5、10、20 μmol/L番茄红素处理HaCaT、HEK细胞24 h后,HaCaT细胞和人原代角质形成细胞中表皮生长因子受体蛋白磷酸化水平和糖皮质激素受体蛋白表达量差异无统计学意义(均P＞ 0.05).结论 番茄红素可降低COLO16细胞活力,抑制表皮生长因子受体蛋白活化,上调糖皮质激素受体表达,这种效应可能有肿瘤细胞特异性.
  • 摘要：目的 探讨结缔组织痣的皮肤镜和反射式共聚焦显微镜(RCM)特征.方法 对1例结缔组织痣患者病程1年以内和1年以上的皮损分别进行皮肤镜和RCM观察,结合组织病理进行分析.结果 结缔组织痣皮肤镜特征:1年以上的皮损中央呈灰白色斑疹,边界清晰,在灰白色斑疹区域可见白色网纹样结构、棕色小球、红斑、点状或逗状血管,有时可见毳毛及毛周围色素环,在白色斑疹周围环绕较均匀的棕褐色色素,皮损境界清晰;1年以内的皮损中央呈灰白色斑疹,边界清晰,在灰白色斑疹区域有少许散在棕褐色色素、毳毛及毛周围色素环.RCM特征:1年以内皮损,表皮大小形状不同的真皮乳头呈环形拥挤在一起,形成蜂窝状或鹅卵石样结构,其真皮乳头环密度和亮度明显增加,真皮胶原纤维束折射增强,可见胶原束与真皮乳头相连接,个别区域交织或呈漩涡状排列;1年以上皮损,由于表皮增厚,Z轴方向2～3 mm深度处组织结构折射光减弱,胶原纤维束不明显,仅见少数淋巴细胞.结论 结缔组织痣有独有的皮肤镜特征,并与组织病理和RCM图像有明确的对应关系.RCM对1年以内的早期皮损诊断有帮助.%Objective To investigate manifestations of connective tissue nevi (CTN) by dermoscopy and reflectance confocal miscroscopy (RCM),and to analyze its pathological features.Methods According to the course of the disease,skin lesions of a patient with CTN were classified into 2 categories:skin lesions of less than 1-year duration and those of more than 1-year duration,which were observed by dermoscopy and RCM,and analyzed by the combination with histopathological examination.Results Dermoscopy imaging of the CTN lesions of more than 1-year duration showed grey-white maculae with clear boundaries in the center of lesions.There were white network structures,brown globules,red spots and punctiform or comma-shaped blood vessels in the gray-white macula area,and vellus hairs and dermal papillary rings could be seen sometimes.The white macula was evenly surrounded by brown to black pigments,and the boundary was clear.For the lesions of less than 1-year duration,gray-white maculae with clear boundaries were also found in the center of the lesions.Besides,there were a few scattered brown to black pigments,vellus hairs and dermal papillary rings in the gray-white macula area.RCM imaging of the lesions of less than 1-year duration or early skin lesions showed dermal papillae of different sizes and shapes crowded with each other and formed a honeycomb or cobblestone-like structure.The density and brightness of the dermal papilla ring obviously increased,and the refraction of the dermal collagen fiber bundles was also enhanced.Some of the dermal collagen fiber bundles were attached to the dermal papilla or intertwined and arranged in a whirlpool pattern.For the lesions of more than 1-year duration,the refraction of the tissue structure was weakened when the depth in Z-axis direction reached 2 to 3 millimetres due to the thickened epidermis,while the dermal collagen fiber bundles were not obvious and only a few lymphocytes could be seen.Conclusions CTN has particular characteristics in dermoscopy,and there is a definite correspondence among dermoscopy features,histopathological findings and RCM features.RCM is helpful for the diagnosis of lesions of less than 1-year duration.
  • 摘要：Objective To evaluate the efficacy and safety of omalizumab on the treatment of chronic spontaneous urticaria (CSU) by systemic review and meta-analysis.Methods Electronic databases,such as PubMed,Clinicaltrials.gov,the Cochrane Database of Systematic Reviews,and the Cochrane Central Register of Controlled Trials,were searched to collect randomized controlled trials (RCTs) about the efficacy and safety of omalizumab in the treatment of CSU.Two reviewers independently screened RCTs according to the inclusion and exclusion criteria,extracted data,and assessed the quality of the included RCTs.And then,a meta-analysis was carried out by using RevMan 5.3 software for comparisons of the efficacy and safety of the 75-,150-,300-,600-mg omalizumab groups versus the placebo group after 1-month treatment,as well as the total omalizumab group versus the placebo group.Results A total of 7 RCTs involving 1 365 patients were included in this meta-analysis.The results showed that the total omalizumab group and different omalizumab subgroups were superior in improving the urticaria activity score of 7 days (UAS7) and wheal number score of 7 days to the placebo group (all P ＜ 0.05).For the improvement in the itch severity score (ISS) of 7 days and complete response rate for main symptoms (UAS7 =0),the total omalizumab group,75-,150-and 300-mg omalizumab groups were superior to the placebo group (all P ＜ 0.05),but there were no significant differences between the 600-mg omalizumab group and the placebo group (P =0.07).The dermatology life quality index (DLQI) was better in the total omalizumab group,150-and 300-mg omalizumab groups than in the placebo group (all P ＜ 0.05),but no significant difference was observed between the 75-mg omalizumab group and the placebo group (P =0.50).There were no significant differences in the incidence of common adverse events or serious adverse events between the total omalizumab group as well as the 75-,150-and 300-mg omalizumab subgroups and the placebo subgroup (all P ＞ 0.05).Conclusions Omalizumab can improve clinical symptoms and life quality of patients with CSU,and is effective in improving the UAS,ISS,wheal number score,DLQI and complete response rate for main symptoms (UAS =0) with high safety.Subcutaneous injection of omalizumab at a dose of 150 or 300 mg/month shows the best efficacy in improving the clinical symptoms and life quality of patients with CSU.%目的 系统评价(Meta分析)奥马珠单抗治疗慢性自发性荨麻疹(CSU)的疗效及安全性.方法 在PubMed、Clinicaltrials.gov、the Cochrane Database of Systematic Reviews和the Cochrane Central Register of Controlled Trials等数据库搜索关于奥马珠单抗治疗CSU疗效和安全性的随机对照临床试验(RCT).2位系统评价员根据纳入与排除标准独立筛选文献,提取资料并评价纳入RCT的质量后,采用RevMan 5.3软件进行Meta分析,比较奥马珠单抗75、150、300、600 mg(1个月)剂量亚组与安慰剂组之间及合并剂量后的奥马珠单抗组与安慰剂组之间的疗效及安全性.结果 共纳入7个RCT,共1 365例病例.结果显示,奥马珠单抗组及各剂量亚组在改善1周荨麻疹活动评分(UAS7)、1周风团数量评分方面均优于安慰剂组(P＜0.05);奥马珠单抗组及75、150、300 mg亚组在改善1周瘙痒严重程度评分(ISS)、主要症状完全控制率方面优于安慰剂组(P＜0.05),尚不能认为600 mg亚组优于安慰剂组(P=0.07);奥马珠单抗组及150、300 mg亚组在改善皮肤病生活质量指数(DLQI)方面均优于安慰剂组(P＜0.05),尚不能认为75 mg亚组优于安慰剂组(P=0.50).奥马珠单抗组及各剂量亚组的一般不良事件发生率、严重不良事件发生率与安慰剂组差异均无统计学意义(P＞0.05).结论 奥马珠单抗可改善CSU患者的临床症状及生活质量,在改善UAS、ISS、风团数量评分、DLQI和控制主要症状(UAS=0)方面均有效,安全性高;皮下注射150 mg/月或300 mg/月奥马珠单抗改善CSU患者临床症状及生活质量的效果最佳.
  • 摘要：Objective To evaluate the effect of aminolevulinic acid-based photodynamic therapy (ALA-PDT) on the expression of Toll-like receptor 2 (TLR2) and downstream signaling pathway molecules,and secretion of cytokines in murine RAW264.7 cells.Methods The RAW264.7 murine macrophages were induced by inactivated Propionibacterium acnes suspension for the establishment of a cell model of inflammation.The cultured RAW264.7 cells were divided into 5 groups:blank control group receiving normal culture followed by the treatment with phosphate buffer saline (PBS),model group treated with inactivated Propionibacterium acnes suspension followed by the treatment with PBS,and three ALA groups treated with inactivated Propionibacterium acnes suspension followed by the treatment with 0.03,0.06 and 0.12 mmol/L ALA,respectively,and infrared radiation at a dose of 16 J/cm2.Enzyme-linked immunosorbent assay (ELISA) was performed to detect levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the culture supematant of RAW264.7 cells,and Western blot analysis to determine the protein expression of TLR2 and myeloid differentiation factor 88 (MyD88),as well as p38,c-Jun N-terminal kinase (JNK),extracellular signal-regulated kinase (ERK),inhibitor of κB kinase α (IκBα) and their phosphorylated forms (p-p38,p-JNK,p-ERK and p-IκBα).Results Compared with the blank control group,the model group showed significantly higher levels of TNF-α ([0.34 ± 0.02] ng/L,P ＜ 0.01) and IL-6 ([0.21 ± 0.03] ng/L,P ＜ 0.05).Compared with the 0.03 mmol/L ALA group,the 0.12 mmol/L ALA group showed a similar level of TNF-α ([0.03 ± 0.01] ng/L,P ＞ 0.05),but a significantly lower level of IL-6 ([0.07 ± 0.01] ng/L,F =114.813,P ＜ 0.01).The protein expression of TLR2,MyD88,p-p38,p-IκBα,p-JNK and p-ERK was all significantly higher in the model group (0.90 ± 0.14,1.11 ± 0.13,0.84 ± 0.04,1.45 ± 0.20,2.56 ± 0.06,3.70 ± 0.40) than in the blank control group (all P ＜ 0.01),and gradually decreased along with the increase of ALA concentration in a dose-dependent manner.Conclusion Photodynamic therapy can suppress the expression of TLR2 in RAW264.7 murine macrophages,and decrease the secretion of cytokines likely by the TLR2 signaling pathway.%目的 探讨氨基酮戊酸光动力疗法(ALA-PDT)对小鼠RAW264.7细胞Toll样受体(TLR)2表达、下游信号转导通路分子和细胞因子分泌的影响.方法 用灭活痤疮丙酸杆菌诱导小鼠RAW264.7巨噬细胞株产生炎症模型,实验分为空白组和模型组,予不同浓度ALA孵育细胞,并予16 J/cm2红光照射.采用酶联免疫吸附法(ELISA)检测细胞培养上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)浓度.Western印迹检测TLR2、髓样分化因子88(MyD88)、磷酸化p38、JNK、ERK、IκBα蛋白表达.采用SPSS 18.0统计软件,各组间计量资料比较采用单因素方差分析或Tambane's T2检验.结果 模型组TNF-α、IL-6的浓度分别为(0.34±0.02) ng/L(P＜ 0.01)、(0.21±0.03) ng/L(P＜ 0.05),均高于空白组.与0.03 mmol/L ALA组比较,0.12 mmol/L ALA组TNF-α浓度为(0.03±0.01) ng/L,差异无统计学意义(P＞0.05),IL-6浓度为(0.07±0.01) ng/L,差异有统计学意义(F=114.813,P＜ 0.01).模型组TLR2、MyD88、p-p38、p-IκBα、p-JNK、p-ERK蛋白表达水平(A值)分别为0.90±0.14(P＜ 0.01)、1.11±0.13(P＜ 0.01)、0.84±0.04(P＜ 0.01)、1.45±0.20(P＜ 0.01)、2.56±0.06(P＜ 0.01)、3.70±0.40(P＜ 0.01),均高于空白组,差异有统计学意义,并随着ALA浓度升高而降低,呈剂量依赖性.结论 光动力治疗可减少小鼠巨噬细胞株RAW264.7细胞TLR2的表达,可能通过细胞信号转导通路,引起细胞因子分泌减少发挥作用.
  • 摘要：Objective To investigate the roles of Dectin-1 in phagocytosis of Candida albicans (C.albicans) by macrophage-like cells derived from a human acute monocytic leukemia cell line THP-1.Methods THP-1 macrophage-like cells served as the target cells,and were transfected with small interfering RNA (siRNA) targeting Dectin-1 to down-regulate the expression of Dectin-1 receptor (siRNA-Dectin-1 group).THP-1 macrophage-like cells transfected with nonsense siRNA (siRNA-NC) served as a negative control group.After transfection,the THP-1 macrophage-like cells in the above 2 groups were cocultured with heat-killed C.albicans separately.And then,fluorescence microscopy was performed to count THP-1 macrophage-like cells phagocytosing C.albicans,and flow cytometry was used to determine the mean fluorescence intensity (MFI) of dihydrorhodamine (DHR)-123 fluorescent cells.Statistical analysis was done by one-way analysis of variance (ANOVA) and t test with the SPSS19.0 software.Results After transfection with siRNA-Dectin-1,the mRNA and protein expression of Dectin-1 significantly decreased in THP-1 macrophage-like cells (t =26.163,P ＜ 0.001).After 1-,2-,4-hour co-culture of THP-1 macrophagelike cells with C.albicans,fluorescence microscopy showed that the phagocytosis rates of C.albicans by THP -1 macrophage-like cells were significantly lower in the siRNA-Dectin-1 group than in the negative control group (17.5％ vs.22.1％,18.6％ vs.24.3％,39.2％ vs.59.1％,respectively,all P ＜ 0.05),so were the percentage of THP-1 macrophage-like cells phagocytosing more than 3 C.albicans cells (2.2％ vs.4.7％,2.5％ vs.5.4％,5.1％ vs.8.3％,respectively,all P ＜ 0.05).After 30-minute,1-,2-and 4-hour co-culture of THP-1 macrophage-like cells with DHR-123-labelled C.albicans,flow cytometry showed that the MFI of C.albicans-phagocytosing cells was significantly lower in the siRNA-Dectin-1 group than in the negative control group (36.8 vs.45.7,54.3 vs.62.4,72.1 vs.84.9,93.6 vs.116.7,respectively,all P ＜ 0.05).Conclusion Dectin-1 receptor plays an important role in the phagocytosis of C.albicans by macrophages.%目的 探讨Dectin-1受体在源于人急性单核细胞白血病细胞(THP-1)的巨噬细胞样细胞吞噬白念珠菌中的作用.方法 以THP-1巨噬细胞样细胞为靶细胞,siRNA靶向下调Dectin-1受体表达,并与热灭活白念珠菌共培养,通过荧光显微镜计数、流式细胞仪检测其对白念珠菌的吞噬作用.应用SPSS19.0统计软件,采用单因素方差分析及t检验进行统计分析.结果 细胞转染siRNA-Dectin-1后,Dectin-1 mRNA相对表达量及蛋白表达水平明显降低(t=26.163,P＜0.001).显微镜观察转染siRNA-Dectin-1和siRNA无义片段(NC)的THP-1巨噬细胞样细胞与白念珠菌共培养1、2、4h吞噬率分别为17.5％和22.1％、18.6％和24.3％、39.2％和59.1％,两两比较差异均有统计学意义(P＜0.05);吞噬≥3个菌的细胞百分比分别为2.2％比4.7％、2.5％比5.4％、5.1％比8.3％,两两比较差异均有统计学意义(P＜0.05).流式细胞仪检测显示,THP-1巨噬细胞样细胞与带有荧光的白念珠菌共培养30 min、1h、2h、4h后,转染siRNA-Dectin-1组较转染siRNA-NC组平均荧光强度明显降低,分别为36.8和45.7、54.3和62.4、72.1和84.9、93.6和116.7,两两比较差异均有统计学意义(P＜0.05).结论 Dectin-1受体在巨噬细胞吞噬白念珠菌的效应中发挥重要作用.
  • 摘要：Objective To determine the expression of cathepsin L2 (CTSL2)and evaluate its activity in skin lesions of seborrheic keratosis (SK),to observe the ultrastructural changes of melanosomes in the skin lesions of SK,and to estimate the effect of CTSL2 on the degradation of melanosomes.Methods Twenty patients with SK were enrolled from the Department of Dermatology,Renmin Hospital of Wuhan University.The lesional tissue and the perilesional normal skin were biopsied from each patient.Among 15 of the 20 patients,hematoxylin and eosin (HE)staining and Fontana-Masson silver staining were performed to observe the distribution of melanin granules,transmission electron microscopy (TEM)was conducted to observe the ultrastructural changes of melanosomes,and immunohistochemical staining was performed to estimate the cellular proliferative activity.RT-PCR and fluorogenic substrate cleavage assay were performed in the other 5 patients to determine the mRNA expression of CTSL2 and evaluate its activity,respectively.Sucrose density gradient ultracentrifugation was performed to isolate and purify melanosomes from the retinal pigment epithelium (RPE) harvested from a discarded eyeball of a 35-year old male patient with informed consent.The purified melanosomes were incubated with epidermal lysates of SK lesions,and TEM was used to observe the changes in the membrane structure of melanosomes.Statistical analysis was carried out by paired t test,and a P value ＜ 0.05 was considered statistically significant.Results A large number of melanin granules were deposited in SK lesions,while the linear deposition of melanin granules was only seen in the basal layer of the normal skin.TEM showed that the percentage of damaged melanosomes was much higher in the normal skin (49.00％ ± 4.00％) than in the SK lesions (24.33％ ± 3.06％)(t =8.49,P ＜ 0.05).RT-PCR revealed that the mRNA expression and activity of CTSL2 were both significantly lower in the SK lesions than in the normal skin (mRNA:0.35 ± 0.09 vs.0.43 ± 0.08,t =3.17,P ＜ 0.05;activity:17.46 ± 0.45 vs.28.78 ± 0.58,t =34.29,P ＜ 0.05).Moreover,TEM also showed that the percentage of damaged melanosome was lower in the SK lesion lysate-treated group (32.33％ ± 4.93％) than in the normal skin lysate-treated group (43.00％ ± 2.65％,t =3.30,P ＜ 0.05).Conclusion Decreased expression of CTSL2 in the SK lesions can affect the degradation of melanosomes by keratinocytes.However,whether CTSL2 directly takes part in the pathogenesis of SK or not is still needed to be further confirmed.%目的 检测脂溢性角化病(SK)皮损中组织蛋白酶L2(CTSL2)表达及活性,观察SK皮损中黑素小体超微结构的变化,研究CTSL2对黑素小体降解的影响.方法 2016年1-8月招募武汉大学人民医院皮肤科门诊SK患者20例,取患者皮损和周围正常皮肤组织,其中15例用HE染色、Fontana-Masson嗜银染色观察黑素颗粒分布,透射电镜(TEM)观察黑素小体超微结构变化,Ki67免疫组化染色检测细胞增殖状态.5例用RT-PCR和荧光底物裂解法分别检测CTSL2 mRNA表达水平及酶活性;用蔗糖梯度离心法从废弃人眼球视网膜色素上皮组织分离纯化黑素小体,并与SK皮损表皮裂解物共孵育,TEM观察孵育后黑素小体膜结构的变化.两组间均数比较采用配对t检验,P＜0.05为差异有统计学意义.结果 SK皮损中可见大量黑素颗粒沉积,而正常皮肤仅在基底层见线形黑素颗粒沉积.TEM观察显示,SK皮损中黑素小体破损率为24.33％±3.06％,正常皮肤为49.00％±4.00％,两组比较,t=8.49,P＜0.05.RT-PCR显示,5例SK皮损中CTSL2 mRNA相对表达水平为0.35±0.09,正常皮肤组织为0.43±0.08,两组比较,t=3.17,P＜0.05.SK皮损中CTSL2酶活性(17.46±0.45)也低于正常皮肤组织(28.78±0.58),t=34.29,P＜0.05.TEM还观察到,SK皮损裂解物处理的黑素小体破损率为32.33％±4.93％,低于正常皮肤43.00％±2.65％,两组比较,t=3.30,P＜0.05.结论 SK皮损中CTSL2表达水平减低影响角质形成细胞对黑素小体的降解,是否直接参与SK皮损的病理发生有待进一步证实.
  • 摘要：Objective To investigate the role of reflectance confocal microscopy (RCM),dermoscopy and VISIA skin detector in the efficacy evaluation of comprehensive treatment of melanosis.Methods Six patients with melanosis were treated with comprehensive therapy,which included avoiding sunlight exposure,sunscreen,moisturizing,intravenous drips of compound glycyrrhizin injection at a singledose of 40 ml once every 3 days for 8 sessions,oral tranexamic acid at a dose of 0.25 g twice a day,oral vitamin E at a dose of 60 mg/d,oral vitamin C at a dose of 0.1 g/d,self-made traditional Chinese medicine in the hospital,topical hydroquinone cream,asiaticoside ointment and levorotatory vitamin C serum.All patients were followed up once every 2 months for 12 months.RCM,dermoscopy and VISIA skin detector were performed in these patients before the treatment and during the follow-up.Statistical analysis was carried out to assess differences in VISIA indices before and after treatment using t test with the SPSS 19.0 software.Results After 12-month treatment,the skin lesions became ligher in color and and smaller in size.Before the treatment,RCM imaging showed disappearance of normal structure at the dermo-epidermaljunction and lots of highly refractive cells of various shapes,which were consistent with histopathological liquefaction degeneration in the basal layers.There were many mildly to moderately refractive round or oval cells in the superficial dermis,which were consistent with melanophages and lymphocytes in histopathology.After 12-month treatment,liquefaction degeneration and infiltration of inflammatory cells were both markedly improved.Before the treatment,dermoscopy showed gray-brown dots or small balls with a grid-like appearance,and indistinct blood vessel shadows.After 2-to 4-month treatment,the number of gray-brown dots or small balls obviously decreased,and linear,branched or reticular blood vessels can be observed.After 12-month treatment,there were no obvious dark brown dots/small balls or blood vessels by dermoscopy.The VISIA brown spot index and erythema index before the treatment were both significantly decreased compared with those after 12-month treatment (brown spot index:47.7 ± 0.9 vs.42.3 ± 0.4,t =6.740,P =0.001;erythema index:36.2 ± 2.1 vs.32.8 ± 1.4,t =6.69,P =0.001).Conclusion The comprehensive therapy of melanosis is effective.RCM,dermoscopy and VISIA skin detector can assist in the evaluation of curative effect on melanosis.%目的 探讨反射式共聚焦显微镜(RCM)、皮肤镜和VISIA皮肤检测仪在评估综合疗法治疗黑变病疗效中的作用.方法 收集2015年3月至2016年10月杭州市第三人民医院皮肤科黑变病患者6例,采用综合疗法治疗,即避光、防晒、保湿,静脉滴注复方甘草酸苷注射液40 ml/次,每3天1次,连续使用8次,口服氨甲环酸片0.25 g每日2次、维生素E 60 mg/d、维生素C 0.1 g/d、自制中药,外用氢醌乳膏、积雪苷霜软膏、左旋维C精华液.每2个月随访1次,随访12个月.治疗前及每次随访时均使用RCM、皮肤镜及VISIA皮肤检测仪检测.采用SPSS 19.0统计学软件,治疗前后指标比较采用t检验.结果 治疗12个月后,6例患者皮损颜色均变淡,面积变小.治疗前RCM表现为真表皮交界处正常结构消失,可见较多强折光形态各异的细胞,对应组织病理上基底层液化变性;真皮浅层有较多轻至中度折光的圆形/椭圆形细胞,对应组织病理上噬黑素细胞及淋巴细胞,治疗12个月后液化变性及炎症细胞浸润均明显改善.治疗前皮肤镜下可见灰褐色小点/小球,呈假网格状外观,隐约可见血管影,治疗2～4个月后灰褐色小点/小球明显减少,可见线状、分枝状或网状血管,治疗12个月后镜下未见明显灰褐色小点/小球及血管.VISIA棕色斑指数治疗前为47.7±0.9,治疗12个月后为42.3±0.4,治疗前后比较,t=6.740,P=0.001;VISIA红色区指数治疗前为36.2±2.1,治疗12个月后为32.8±1.4,治疗前后比较,t=6.69,P=0.001.结论 综合疗法治疗黑变病有效,RCM、皮肤镜和VISIA皮肤测试仪可辅助评估疗效.
  • 摘要：Objective To evaluate effects of targeted silencing of growth arrest and DNA damage inducible gene 45α (Gadd45α) by small interference RNA (siRNA) on the invasion and migration of a cutaneous squamous cell carcinoma cell line A431.Methods Real-time fluorescence-based quantitative PCR and Western blot analysis were performed to detect the mRNA and protein expression of A431 and Colon16 cells respectively,and then A431 cells with highly expressed Gadd45α served as a research object.Cultured A431 cells were divided into 3 groups:experimental group transfected with Gadd45α-siRNA-1,negative control group transfected with negative control siRNA,and blank control group receiving no treatment.After the RNA interference,the mRNA and protein expression of Gadd45α in the above 3 groups were measured by real-time fluorescence-based quantitative PCR and Western blot analysis,respectively,and the effect of the RNA interference on the invasion and migration abilities of A431 cells was evaluated by Transwell and wound scratch assays.Results Gadd45α mRNA and protein were highly expressed in the A431 cells.After the RNA interference,the experimental group showed markedly lower mRNA and protein expressions of Gadd45 in the A431 cells (0.286 ± 0.013,0.33 ± 0.007,respectively) compared with the negative control group (1.028 ± 0.183,0.87 ± 0.002,respectively)and blank control group (1.001 ± 0.057,0.86 ± 0.004,respectively),and there were significant differences in the mRNA and protein expressions of Gadd45 among the 3 groups (F =5 893.857,2 763.000,both P ＜ 0.001).The number of A431 cells crossing the polycarbonate membrane was higher in the experimental group (66.33 ± 3.79) than in the negative control group (26.00 ± 4.36) and the blank control group (28.33 ± 4.16),and there was a significant difference among the 3 groups (F =20.084,P =0.002).Moreover,the wound scratch assay showed that the number of migrating A431 cells per high-power field was significantly higher in the experimental group than in the negative control group and the blank control group (315.33 ± 6.66,154.67 ± 2.08,130.67 ± 3.51 respectively;F =1 676.255,P ＜ 0.001).Conclusion Gadd45α is highly expressed in the cutaneous squamous cell carcinoma cell line A431,and targeted silencing of Gadd45α gene can enhance the in vitro invasion and migration abilities of A431 cells.%目的 探讨靶向沉默生长抑制和DNA损伤诱导45蛋白α(Gadd45α)对皮肤鳞状细胞癌(简称鳞癌)细胞株A431侵袭和迁移的影响.方法 采用实时荧光定量PCR、Western印迹检测A431和Colon16细胞Gadd45α基因和蛋白的表达,选取高表达Gadd45α的A431细胞株作为研究对象.将A431细胞分为实验组、阴性对照组和空白对照组:实验组转染Gadd45α-siRNA-1,阴性对照组转染阴性对照siRNA,空白对照组不做任何处理.采用实时荧光定量PCR和Western印迹法分别检测干预后Gadd45α mRNA和蛋白的表达,Transwell小室法及细胞划痕法检测干预对A431细胞株侵袭及迁移能力的影响.结果 A431细胞高表达Gadd45α基因和蛋白.干预后实验组A431细胞Gadd45α mRNA及蛋白相对表达量分别为0.286±0.013、0.33±0.007,明显低于阴性对照组组(1.028±0.183、0.87±0.002)以及空白对照组(1.001±0.057、0.86±0.004),差异均有统计学意义(F值分别为5 893.857、2 763.000,均P＜0.001).实验组A431细胞株穿过聚碳酸酯膜的细胞数为66.33±3.79,明显多于阴性对照组(26.00±4.36)和空白对照组(28.33±4.16),3组间差异有统计学意义(F=20.084,P=0.002).划痕实验中,实验组每高倍视野下细胞迁移数为315.33±6.66,明显多于阴性对照组(154.67±2.08)和空白对照组(130.67±3.51),3组间差异有统计学意义(F=1 676.255,P＜0.001).结论 A431细胞株高表达Gadd45α,靶向沉默Gadd45α表达能增强A431细胞株的侵袭和迁移能力.
  • 摘要：Objective To investigate clinical and histopathological features of cutaneous epithelioid hemangioma (EH).Methods Clinical and histological data were collected from 22 patients with EH and analyzed.Results Of the 22 EH patients,10 were male,and 12 were female.The age at onset ranged from 16 to 62 years,with an average age of 45.91 years.The duration of disease varied from 1 month to 20 years,with an average disease duration of 37 months.Skin lesions most frequently occurred on the scalp in 14 cases (63.6％),followed by the ear in 6 cases (27.3％),the forehead in 2 cases (9.1％),and the thigh in 1 case (4.5％).Lesions affected both the scalp and forehead in 1 case.Histopathological examination of the 22 cases revealed vascular proliferation.The blood vessels were lined with epithelioid endothelial cells,and a large number of lymphocytes and eosinophils infiltrated around the vessels.The dermis were involved in all cases,and subcutaneous tissues were involved in 8 cases (36.4％).Immunohistochemical examination of 6 cases showed positive staining for CD31,CD34 and factor Ⅷ in blood vessel walls.Conclusion EH is an uncommon disease characterized by vascular proliferation and inflammatory infiltration,and clinical manifestations in combination with histopathological examination facilitate its diagnosis.%目的 探讨皮肤上皮样血管瘤的临床表现与组织病理学特征.方法 回顾分析22例上皮样血管瘤患者的临床和病理资料.结果 22例上皮样血管瘤患者中,男10例,女12例;发病年龄16～62岁,平均45.91岁;病程1个月至20年,平均37个月.皮损位于头部14例(63.6％),耳部6例(27.3％),额部2例(9.1％),大腿部1例(4.5％),其中1例同时累及头皮及额部.组织病理学检查:22例均表现为血管增生,内衬上皮样内皮细胞,管周大量淋巴细胞及嗜酸性粒细胞浸润.所有皮损均累及真皮层,另外皮下组织受累8例(36.4％).6例免疫组化显示,血管壁CD31、CD34、Ⅷ因子均为阳性.结论 上皮样血管瘤是一种少见的以血管增生和炎症浸润为主要特征的皮肤疾病,需要临床结合组织病理检查进行诊断.
  • 摘要：目的 报告1例TSC2基因发生体细胞镶嵌突变导致结节性硬化症患者的临床特点和基因突变情况.方法 采集1例临床拟诊断结节性硬化症患者及其父母外周血提取基因组DNA,采用聚合酶链反应(PCR)及第二代测序法扩增TSC1和TSC2基因的全部外显子及其侧翼序列,并进行DNA测序分析,查找基因突变位点,以200例无关健康人DNA作为对照.提取患者皮损处皮肤组织DNA,利用PCR扩增TSC2基因目的片段并测序.结果 患者临床出现面部血管纤维瘤、腰部色素减退斑、甲周纤维瘤及肾血管平滑肌脂肪瘤,符合结节性硬化症的诊断标准.患者TSC2基因存在c.5130_5131insT突变(p.V1711Cfs*18),且瘤体DNA较外周血DNA携带更高频率突变.患者父母、无关正常对照及公开数据库均未发现该突变.结论 TSC2基因发生体细胞镶嵌突变c.5130_5131insT是导致该患者较轻结节性硬化症表型的原因.%Objective To investigate clinical features and detect mutations in a case of tuberous sclerosis complex (TSC) caused by a somatic mosaic mutation in the TSC2 gene.Methods Peripheral blood samples were obtained from a patient with suspected TSC,his parents,and 200 unrelated healthy controls.Genomic DNA was extracted from these blood samples,polymerase chain reaction (PCR)and nextgeneration sequencing were performed to amplify all the exons and their flanking sequences of the TSC 1 and TSC2 genes followed by DNA sequencing,so as to identify mutations in the TSC 1 and TSC2 genes.DNA was also extracted from lesional skin tissues of the patient,and PCR was conducted to amplify the target fragment of the TSC2 gene followed by DNA sequencing.Results The patient clinically presented with facial angiofibroma,depigmented patches on the waist,periungual fibroma and angioleio-myolipoma of the kidney,which were consistent with the diagnosis of TSC.A mutation c.5130_5131insT(p.V1711Cfs* 18) was identified in the TSC2 gene in the patient.A higher frequency of the mutation was found in the DNA of the tumor tissue than in that of the peripheral blood.No such a mutation was found in his parents'DNA,unrelated healthy controls or any public database.Conclusion The somatic mosaic mutation c.5130_513 1insT in the TSC2 gene is responsible for the phenotype of TSC in the patient.
  • 摘要：目的 评价菌落PCR在快速鉴定头癣病原菌中的可靠性及临床实用性.方法 于2016年1月至2017年3月在无锡市第二人民医院皮肤科门诊收集儿童头癣病例17例,采用菌落PCR技术检测病原菌,同时与常规PCR及形态学鉴定结果进行比较,评价菌落PCR应用于头癣病原菌鉴定的可靠性.结果 17例儿童头癣患者临床标本经培养收集菌种、菌落PCR均扩增成功,从断发或皮屑标本中取材至DNA模板制备成功耗时(3.82±0.50)d,较传统形态学鉴定(14d)明显缩短.以常规PCR鉴定结果为标准,菌落PCR菌种鉴定正确率为100％,优于传统的形态学鉴定(正确率88.2％).结论 菌落PCR可用于临床头癣病原菌的检测,是一种快速、经济、可靠的分子检测技术.%Objective To evaluate the reliability and clinical practicality of colony PCR in rapid detection of pathogenic fungi causing tinea capitis.Methods Totally,17 children with tinea capitis were enrolled from the Department of Dermatology of Wuxi No.2 People's Hospital between January 2016 and March 2017.Colony PCR was performed to detect pathogenic fungi.The results of colony PCR were compared with those of routine PCR and morphological identification,so as to evaluate the reliability of colony PCR in the identification of pathogenic fungi causing tinea capitis.Results After clinical specimens (broken hairs and scales) from the 17 patients were subjected to fungal culture,the mycelia were collected and successfully amplified by colony PCR.The time of clony PCR (mean,3.82 ± 0.50 days) for DNA template preparation was short than that of traditional morphological identification (14 days).Based on the results of conventional PCR,the accuracy of colony PCR for fungal identification was 100％,which was superior to that of conventional PCR (88.2％).Conclusions Colony PCR can be applied to the clinical detection of pathogenic fungi causing tinea capitis at specy level,and is a kind of rapid,economic and reliable molecular detection technique.

    中华皮肤科杂志的期刊信息

    • 创刊时间：1953
    • 地区：CN
    • 语言：中文
    • 热门主题：中华医学会
    • 学科分类： 皮肤病学与性病学;

    中华皮肤科杂志的获奖情况

    • • 2002年获第三届中国科协优秀科技期刊二等奖
    • • 2002年获华东地区第三届优秀期刊奖，同年在首届“江苏期刊方阵”评选中被评为双十佳期刊。
    • • 2000年本刊获中华医学会85周年优秀期刊银奖。

    中华皮肤科杂志的收录情况

    • • 北大核心期刊（1992版）
    • • 北大核心期刊（1996版）
    • • 北大核心期刊（2000版）
    • • 北大核心期刊（2004版）
    • • 北大核心期刊（2008版）
    • • 北大核心期刊（2011版）
    • • 北大核心期刊（2014版）
    • • 北大核心期刊（2017版）
    • • 农业与生物科学研究中心文摘
    • • 化学文摘（网络版）
    • • 中国科学引文数据库（2011-2012）
    • • 中国科学引文数据库（2013-2014）
    • • 中国科学引文数据库（2015-2016）
    • • 中国科学引文数据库（2017-2018）
    • • 中国科学引文数据库（2019-2020）
    • • 日本科学技术振兴机构数据库
    • • 中国科技核心期刊

    中华皮肤科杂志的投稿信息

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