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首页> 外文期刊>Chemosphere >Photodynamic effects of 31 different phthalocyanines on a human keratinocyte cell line
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Photodynamic effects of 31 different phthalocyanines on a human keratinocyte cell line

机译：31种酞菁对人角质形成细胞系的光动力效应

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摘要

Phthalocyanines (Pcs, colored macromolecular compounds with the ability to generate singlet oxygen) represent a promising group of photosensitizers due to their intense absorption in the red and UV portion of the spectrum which leads to their excitation. In order to characterize possible toxic effects associated with eventual practical use and application of these chemicals, we employed an in vitro cell culture model to evaluate cytotoxic effects of 31 different phthalocyanines using neutral red uptake assay. An immortalized human keratinocyte cell line HaCaT was exposed to the tested chemicals for 2 or 24 h, either with or without illumination in the last 60 min of the exposure period. After 2- or 24-h exposure without illumination, no cytotoxic effects or weak cytotoxic effects were induced by any Pc under the study and EC50 values could not be obtained within the tested concentration ranges (1.25-20 mg L~(-1) or 0.625-10 mg L~(-1)). On the other hand, exposure to phthalocyanines under illumination induced a significant cytotoxic effect. The most pronounced cytotoxicity was elicited by Pcs previously shown to have high positive charge densities at peripheral parts of substituent groups, which is most likely the factor responsible for the binding of Pc to negatively charged membranes on the cell surface and thus guaranteeing the tight connection necessary for the singlet oxygen attack on the cell surface.
机译：酞菁类化合物（Pcs，具有产生单线态氧的能力的有色大分子化合物）代表了一组有前景的光敏剂，因为它们在光谱的红色和紫外线部分被强烈吸收，从而导致它们被激发。为了表征与这些化学品的最终实际使用和应用相关的可能的毒性作用，我们采用了一种体外细胞培养模型，使用中性红吸收试验评估了31种不同的酞菁的细胞毒性作用。将永生化的人角质形成细胞系HaCaT暴露于受试化学物质2或24小时，在暴露期的最后60分钟内进行光照或不光照。在没有光照的情况下暴露2或24小时后，研究中的任何Pc均未诱导出细胞毒性作用或弱的细胞毒性作用，并且在测试浓度范围（1.25-20 mg L〜（-1）或0.625-10 mg L〜（-1））。另一方面，在光照下暴露于酞菁会引起明显的细胞毒性作用。 Pcs引起的最明显的细胞毒性是先前显示的，它在取代基的外围部分具有较高的正电荷密度，这很可能是造成Pc与细胞表面带负电的膜结合的因素，从而保证了必要的紧密连接用于单线态氧攻击细胞表面。

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