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首页> 外文期刊>Chemosphere >Effects of estrone on the early life stages and expression of vitellogenin and estrogen receptor genes of Japanese medaka (Oryzias latipes)
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Effects of estrone on the early life stages and expression of vitellogenin and estrogen receptor genes of Japanese medaka (Oryzias latipes)

机译：雌酮对的早期生长及卵黄蛋白原和雌激素受体基因表达的影响

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摘要

The fertilized eggs of Japanese medaka (Oryzias latipes) were exposed to estrone (E1) at 5-5000 ng L~(-1) for 15 d, and the hatched fry were exposed continuously to the same concentrations for the additional 15 d. Adverse effects on hatchability, time to hatching, and gross abnormalities occurred at 50 ng L~(-1) or above. Then the fry were divided into a continual exposure group, and a water recovery group. When the fry were exposed to E1 for another 60 d, there was a decrease in the hepatosomatic index (HSI) of males and the influence disappeared in the water recovery group. The gonadosonatic index (GSI) of females at 500 ng L~(-1) decreased significantly in another 60 d exposure. While the fry were maintained in dechlorinated tap water for 60 d, a significant decrease in female GSI was observed at 50 ng L~(-1) or above. An increased GSI was found in males in both continual exposure and water recovery groups at all E1 treatments. Quantitative RT-PCR showed that vitellogenin-Ⅰ (Vtg-Ⅰ) gene expressions in the female liver were significantly down-regulated at 50 ng L~(-1) in the continual exposure group, and at 500 ng L~(-1) in the water recovery group, while male Vtg-Ⅰ genes were significantly up-regulated for all E1 treatments. In addition, all E1 treatments caused sex reversal of males. These results suggest that E1 at 5 ng L~(-1) or above have unrecoverable impacts on the gonadal growth and development of medaka, even if only early life stages were exposed to E1.
机译：将日本高加索（Oryzias latipes）的受精卵在5-5000 ng L〜（-1）下暴露于雌酮（E1）15 d，再将孵化的鱼苗连续暴露于相同浓度下15 d。在50 ng L〜（-1）或以上时，会对孵化率，孵化时间和严重异常产生不利影响。然后将鱼苗分为连续暴露组和水回收组。当鱼苗再暴露于E1 60天后，雄性的肝体指数（HSI）下降，并且在水回收组中影响消失了。 500 ng L〜（-1）处的雌性性腺激素指数（GSI）在再暴露60 d后显着下降。在将鱼苗在脱氯自来水中保持60 d的同时，在50 ng L〜（-1）或更高浓度时，雌性GSI显着下降。在所有E1处理下，持续暴露组和水分回收组的男性中GSI均升高。定量RT-PCR显示，连续暴露组在50 ng L〜（-1）和500 ng L〜（-1）时，女性肝脏中的卵黄蛋白原-Ⅰ（Vtg-Ⅰ）基因表达显着下调。在水回收组中，雄性Vtg-Ⅰ基因在所有E1处理中均显着上调。此外，所有E1治疗都会导致男性性逆转。这些结果表明，即使只有早期生命阶段暴露于E1，E1在5 ng L〜（-1）或更高的水平对on的性腺生长和发育也没有可恢复的影响。

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  • 来源
    《Chemosphere》 |2013年第6期|1104-1110|共7页
  • 作者

    Bingli Lei; Yu Wen; Xuetong Wang; Jinmiao Zha; Wei Li; Zijian Wang; Yanfeng Sun; Jia Kang; Yipei Wang;

  • 作者单位

    Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Nanchen Rd 333, Baoshan District, Shanghai 200444, PR China;

    Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Nanchen Rd 333, Baoshan District, Shanghai 200444, PR China;

    Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Nanchen Rd 333, Baoshan District, Shanghai 200444, PR China;

    State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China;

    State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China;

    State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China;

    Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Nanchen Rd 333, Baoshan District, Shanghai 200444, PR China;

    Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Nanchen Rd 333, Baoshan District, Shanghai 200444, PR China;

    Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Nanchen Rd 333, Baoshan District, Shanghai 200444, PR China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Estrone; Early life stage; Vitellogenin gene; Estrogen receptor gene; Oryzias latipes; Water recovery group;

    机译：雌酮生命早期阶段;卵黄蛋白原基因;雌激素受体基因;稻（Oryzias latipes）;水回收组;

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