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首页> 外文期刊>Chemosphere >Bulk soil and rhizosphere bacterial community PCR-DGGE profiles and β-galactosidase activity as indicators of biological quality in soils contaminated by heavy metals and cultivated with Silene vulgaris (Moench) Garcke
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Bulk soil and rhizosphere bacterial community PCR-DGGE profiles and β-galactosidase activity as indicators of biological quality in soils contaminated by heavy metals and cultivated with Silene vulgaris (Moench) Garcke

机译：土壤和根际细菌群落的PCR-DGGE图谱和β-半乳糖苷酶活性，作为被重金属污染并用寻常硅藻（Moench）Garcke培育的土壤中生物质量的指标

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摘要

The biological quality of two heavy metal contaminated soils (soil C: Typic Calcixerept, pH 8.3 and soil H: Typic Haploxeraf, pH 7.3) was investigated after growing the metal-tolerant plant Silene vulgaris (Moench) Garcke for two vegetative periods. The activity of the enzyme p-galactosidase, which is sensitive to the presence of contaminants in soil, and the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiles of 16S rRNA gene fragments of culturable bacteria from bulk soil and rhizosphere were determined. The microbial enzymatic activity was higher in planted soils than in bare soils at the contamination level of 600 mg of total heavy metals kg~(-1) soil. After growing S. vulgaris, β-galactosidase activity was almost recovered in the calcareous soil. In this soil new bands appeared in the PCR-DGGE profiles of the rhizosphere bacterial community as a response to the exposure to heavy metals.
机译：在将耐金属性植物寻常硅（Moench）Garcke生长两个营养期后，研究了两种重金属污染土壤（土壤C：Typic Calcixerept，pH 8.3和土壤H：Typic Haploxeraf，pH 7.3）的生物质量。对土壤中污染物的存在敏感的p-半乳糖苷酶的活性，以及​​来自土壤和根际的可培养细菌的16S rRNA基因片段的聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）谱图决心。在土壤重金属总量为600 mg kg〜（-1）的情况下，种植土壤中的微生物酶活性高于裸露土壤。寻常葡萄球菌生长后，钙质土壤中的β-半乳糖苷酶活性几乎恢复。在这种土壤中，根际细菌群落的PCR-DGGE图谱中出现了新的条带，作为对重金属暴露的响应。

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  • 来源
    《Chemosphere》 |2009年第10期|1376-1381|共6页
  • 作者

    M.J. Martinez-Inigo; A. Perez-Sanz; I. Ortiz; J. Alonso; R. Alarcon; P. Garcia; M.C. Lobo;

  • 作者单位

    Instituto Madrileno de Investigation y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Km, 38.2 A-11, 28800 Madrid, Spain;

    Instituto Madrileno de Investigation y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Km, 38.2 A-11, 28800 Madrid, Spain;

    Instituto Madrileno de Investigation y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Km, 38.2 A-11, 28800 Madrid, Spain;

    Instituto Madrileno de Investigation y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Km, 38.2 A-11, 28800 Madrid, Spain;

    Instituto Madrileno de Investigation y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Km, 38.2 A-11, 28800 Madrid, Spain;

    Instituto Madrileno de Investigation y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Km, 38.2 A-11, 28800 Madrid, Spain;

    Instituto Madrileno de Investigation y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Km, 38.2 A-11, 28800 Madrid, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    heavy metals; soil; revegetation; silene vulgaris; rhizosphere; microbial activity;

    机译：重金属;泥;植被恢复寻常的硅根际微生物活性;

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