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首页> 外文期刊>Chemosphere >Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: Linking biomarkers to behaviour
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Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: Linking biomarkers to behaviour

机译：铜和汞对河口鱼类Pomatoschistus微生物的急性影响：将生物标志物与行为联系起来

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摘要

The main objective of the present study was to investigate possible links between biomarkers and swimming performance in the estuarine fish Pomatoschistus microps acutely exposed to metals (copper and mercury). In independent bioassays, P. microps juveniles were individually exposed for 96 h to sub-lethal concentrations of copper or mercury. At the end of the assays, swimming performance of fish was measured using a device specially developed for epibenthic fish (SPEDE). Furthermore, the following biomarkers were measured: lipid peroxidation (LPO) and the activity of the enzymes acetylcholinesterase (AChE), lactate dehydrogenase (LDH), glutathione S-transferases (GST), 7-ethoxyresorufin-O-deethylase (EROD), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione peroxi-dase (GPx). LC_(50)s of copper and mercury (dissolved throughout metal concentrations) at 96 h were 568 μg L~(-1) and 62 ug L~(-1) respectively. Significant and concentration-dependent effects of both metals on swimming resistance and covered distance against water flow were found at concentrations equal or higher than 50 μg L~(-1) for copper and 3 μg L~(-1) for mercury (dissolved throughout metal concentrations). These results indicate that SPEDE was efficacious to quantify behavioural alterations in the epibenthic fish P. microps at ecologically relevant concentrations. Significant alterations by both metals on biomarkers were found including: inhibition of AChE and EROD activities, induction of LDH, GST and anti-oxidant enzymes, and increased LPO levels, with LOEC values ranging from 25 to 200 μg L~(-1) for copper and from 3 to 25 μg L~(-1) for mercury (dissolved throughout metal concentrations). Furthermore, significant and positive correlations were found between some biomarkers (AChE and EROD) and behavioural parameters, while negative correlations were found for others (LPO, anti-oxidant enzymes and LDH) suggesting that disruption of cholinergic function through AChE inhibition, decreased detoxification capability due to EROD inhibition, additional energetic demands to face chemical stress, and oxi-dative stress and damage may contribute to decrease the swimming performance of fish. Since a reduced swimming capability of fish may reduce their ability to capture preys, avoid predators, and interfere with social and reproductive behaviour, the exposure of P. microps to copper and/or mercury concentrations similar to those tested here may decrease the fitness of wild populations of this species.
机译：本研究的主要目的是调查在急性暴露于金属（铜和汞）的河口鱼类Pomatoschistus显微菌中生物标志物与游泳性能之间的可能联系。在独立的生物测定法中，将肺吸虫的幼体分别暴露于亚致死浓度的铜或汞96小时。分析结束时，使用专门为表皮鱼类开发的设备（SPEDE）测量了鱼类的游泳性能。此外，还测量了以下生物标志物：脂质过氧化（LPO）和乙酰胆碱酯酶（AChE），乳酸脱氢酶（LDH），谷胱甘肽S-转移酶（GST），7-乙氧基异佛手菌素-O-脱乙基酶（EROD），超氧化物酶的活性歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GPx）。铜和汞（在整个金属浓度中溶解）的LC_（50）s在96 h时分别为568μgL〜（-1）和62 ug L〜（-1）。当铜的浓度等于或高于50μgL〜（-1）且汞的浓度等于或高于3μgL〜（-1）时，两种金属对游泳阻力和覆盖距离的阻隔作用均具有显着且与浓度有关的影响（全金属浓度）。这些结果表明，SPEDE可以有效地量化生态学上相关浓度的表皮鱼类小球藻的行为变化。发现两种金属对生物标志物的显着改变包括：抑制AChE和EROD活性，诱导LDH，GST和抗氧化酶以及增加LPO水平，LOEC值在25〜200μgL〜（-1）之间。铜和3至25μgL〜（-1）中的汞（在整个金属浓度中均溶解）。此外，在某些生物标记物（AChE和EROD）与行为参数之间发现了显着正相关，而在其他生物标记物（LPO，抗氧化酶和LDH）之间则发现了负相关，这表明通过抑制AChE破坏胆碱能功能，降低了排毒能力。由于EROD抑制，面对化学应激的额外能量需求以及氧化应激和损害可能会导致鱼类游泳性能下降。由于鱼类游泳能力的下降可能会降低其捕获猎物，避开捕食者以及干扰社交和生殖行为的能力，因此，与此处测试的相似，铜绿假单胞菌暴露于铜和/或汞的浓度可能会降低野生动物的适应性该物种的种群。

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  • 来源
    《Chemosphere》 |2009年第10期|1416-1427|共12页
  • 作者

    L.R. Vieira; C. Gravato; A.M.V.M. Soares; F. Morgado; L. Guilhermino;

  • 作者单位

    CIMAR - LA/CIIMAR-Centro Interdisciplinar de Investigacao Marinha e Ambiental, Laboratorio de Ecotoxicologia, Rua dos Bragas, 289, 4050-123 Porto, Portugal Universidade de Aveiro, CESAM - Centra de Estudos do Ambiente e do Mar, Departamento de Biologia, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal;

    CIMAR - LA/CIIMAR-Centro Interdisciplinar de Investigacao Marinha e Ambiental, Laboratorio de Ecotoxicologia, Rua dos Bragas, 289, 4050-123 Porto, Portugal;

    Universidade de Aveiro, CESAM - Centra de Estudos do Ambiente e do Mar, Departamento de Biologia, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal;

    Universidade de Aveiro, CESAM - Centra de Estudos do Ambiente e do Mar, Departamento de Biologia, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal;

    CIMAR - LA/CIIMAR-Centro Interdisciplinar de Investigacao Marinha e Ambiental, Laboratorio de Ecotoxicologia, Rua dos Bragas, 289, 4050-123 Porto, Portugal ICBAS - Institute de Ciencias Biomedicas de Abel Salazar, Universidade do Porto, Departamento de Estudos de Populacoes, Laboratorio de Ecotoxicologia, Universidade do Porto, Lg. Prof. Abel Salazar, 2, 4099-003 Porto, Portugal;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    pomatoschistus microps; metals; swimming performance; biomarkers;

    机译：胃丝虫金属;游泳表演;生物标志物;

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