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首页> 外文期刊>Chinese Journal of Chemistry >Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC
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Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC

机译：反相高效液相色谱法灵敏地分析注射液中的氨基酸

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摘要

A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobenzene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good repro-ducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis.
机译：描述了一种以1-氟-2,4-二硝基苯为反应试剂的方便的氨基酸衍生方法和分离系统。在特定的化学键合相色谱柱上，通过简单的线性梯度洗脱（由水性缓冲液和甲醇组成）分离衍生氨基酸。用普通的紫外线吸收检测器在360nm处检测洗脱液。氨基酸的检出限低至10皮摩尔。该方法已成功应用于医院中氨基酸注射液的测定。它具有良好的重现性和精度。该程序避免了常规氨基酸分析中使用的特定衍生设备和分析仪的要求。

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