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首页> 外文期刊>Chinese Journal of Traumatology >Effects of chitosan on cell proliferation and collagen production of tendon sheath fibroblasts. epitenon tenocytes, and endotenon tenocytes
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Effects of chitosan on cell proliferation and collagen production of tendon sheath fibroblasts. epitenon tenocytes, and endotenon tenocytes

机译：壳聚糖对肌腱鞘成纤维细胞增殖和胶原生成的影响。表皮肌腱细胞和内膜肌腱细胞

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Objective: To study the proliferation and collagen production of tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes; and the effects of chitosan on cell proliferation and collagen production in the 3 cell types of rabbit flexor tendon. Methods: Three cell lines of tendon sheath, epitenon, and endotenon were isolated from rabbit flexor tendon and cultured. Cell culture media was added with chitosan. The cell number and production of collagens Ⅰ, Ⅱ, and Ⅲ were measured and compared with those cultured without chitosan. The expression of type Ⅰ collagen in tendon sheath fibroblasts was determined by quantitative analysis of reverse-transcription polymerase chain reaction. Results : All 3 cell lines produced collagens Ⅰ, Ⅱ, and Ⅲ. Adding chitosan to cell media resulted in a significant decrease in cell number in all 3 cell lines. In addition, there was a significant decrease in collagens Ⅰ, Ⅱ, and Ⅲ production in all 3 cell lines as well as the expression levels of type Ⅰ collagen in tendon sheath fibroblasts (P < 0.05). Conclusions: Chitosan can inhibit cell proliferation and collagen production of the tendon sheath, epitenon, and endotenon, and may provide a promising approach to obviating tendon adhesion formation clinically.
机译：目的：研究肌腱鞘成纤维细胞，表皮肌腱细胞和内膜肌腱细胞的增殖和胶原蛋白的产生。以及壳聚糖对家兔三种屈肌腱细胞增殖和胶原生成的影响。方法：从兔屈肌腱中分离并培养3个肌腱鞘，上皮肌腱和肌腱细胞系。向细胞培养基中加入壳聚糖。测量胶原Ⅰ，Ⅱ和Ⅲ的细胞数和产量，并与无壳聚糖培养的胶原进行比较。通过定量分析逆转录聚合酶链反应确定肌腱鞘成纤维细胞中Ⅰ型胶原蛋白的表达。结果：3种细胞系均产生Ⅰ，Ⅱ，Ⅲ型胶原。向细胞培养基中添加壳聚糖会导致所有3种细胞系的细胞数显着减少。此外，所有3种细胞系的Ⅰ，Ⅱ，Ⅲ型胶原蛋白产量均明显下降，且腱鞘成纤维细胞中Ⅰ型胶原蛋白的表达水平显着降低（P <0.05）。结论：壳聚糖可抑制肌腱鞘，上皮和内膜的细胞增殖和胶原蛋白的产生，为临床消除肌腱粘连的形成提供了有希望的方法。

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