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首页> 外文期刊>Chinese Medical Journal >Possible function of outward potassium currents in isolated Deiters' cells of guinea pig cochlea
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Possible function of outward potassium currents in isolated Deiters' cells of guinea pig cochlea

机译：豚鼠耳蜗分离的Deiters细胞中向外钾电流的可能作用

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Objective To study potassium currents in isolated Deiters' cells of guinea pig cochlea and explore possible function of potassium current in Deiters' cell. Methods The whole cell patch clamp recording technique was used to study potassium currents of Deiters' cells in normal external solution and solutions with different K~+ concentrations. We also studied the effects on reversal potentials and outward potassium currents. Results Isolated Deiters' cells possess voltage dependent, outwardly rectifying ion channels, which are K~+ selective. 50 mmol/L K~+ and 150 mmol/L K~+ in external solution reduced I_(K-max) from (10.06 +- 2.2)nA (n = 13) in normal external solution to (6.43 +- 1.67)nA (n = 6, P<0.05) and (5.49 +- 1.33)nA (n = 6, P <0.05), respectively. While the amplitude of tailcurrents decreased from (468.76 +- 61.76)pA in 5 mmol K~+ external solution to (224.74 +- 35.89)pA (P<0.05) in 50 mmol/L K~+ and to ( -911.59 +- 78.17)pA (P<0.01) in 150 mmol/L K~+ external solution. Conclusions Outwardly rectifying potassium in Deiters' cells could buffer extracellular K~+ in the small space between Deiters' cells and outer hair cells or neural fibers and participate in the diffusion of K~+ from endolymph to peri lymph.
机译：目的研究豚鼠耳蜗分离的Deiters细胞的钾电流，探讨钾电流在Deiters细胞中的可能作用。方法采用全细胞膜片钳记录技术研究正常外部溶液和不同K〜+浓度溶液中Deiters细胞的钾电流。我们还研究了对逆转电位和向外钾电流的影响。结果分离的Deiters细胞具有依赖电压的向外整流离子通道，这些通道具有K +选择性。外部溶液中的50 mmol / LK〜+和150 mmol / LK〜+将I_（K-max）从常规外部溶液中的（10.06 +-2.2）nA（n = 13）降低为（6.43 +-1.67）nA（n = 6，P <0.05）和（5.49±1.33）nA（n = 6，P <0.05）。尾电流幅值从5 mmol K〜+溶液中的（468.76 +-61.76）pA降至50 mmol / LK〜+溶液中的（224.74 +-35.89）pA（P <0.05）和（-911.59 +-78.17）在150 mmol / LK〜+外部溶液中的pA（P <0.01）。结论外向整流钾可在外膜与外毛细胞或神经纤维之间的小间隙中缓冲细胞外K〜+，并参与K〜+从内淋巴到周围淋巴的扩散。

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