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Multiple splicing types of OsRIX4, an RAD21 homolog in rice (Oryza sativa L.)

机译:水稻(Oryza sativa L.)的RAD21同源物OsRIX4的多种剪接类型

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摘要

The present paper describes multiple splicing types of OsRIX4, an RAD21 homolog in rice. A type of alternative splicing (AS), distinctive from all five previously known splicing types, was identified in which interior sequences of a constitutive exon could be spliced. Translation of the transcript produced with this AS type was demonstrated at the protein level. Expression of multiple transcripts was organ specific. The expression abundance of transcripts, OsRIX4-4 and OsRIX4-5, was positively correlated with fertility in rice. The splicing type identified in the present study provided the means to further understand and define different mRNA splicing types in plants and suggested that post-transcription processing of mRNA and its regulation mechanism are complex.
机译:本文描述了水稻中RAD21同源物OsRIX4的多种剪接类型。确定了一种与所有五个先前已知的剪接类型不同的替代剪接(AS)类型,其中可以剪接本构外显子的内部序列。在蛋白质水平上证实了这种AS类型产生的转录本的翻译。多种转录物的表达是器官特异性的。转录本OsRIX4-4和OsRIX4-5的表达丰度与水稻的育性正相关。本研究中鉴定的剪接类型为进一步理解和定义植物中不同的mRNA剪接类型提供了手段,并提示mRNA的转录后加工及其调控机制是复杂的。

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