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首页> 外文期刊>Chinese science bulletin >Transfer of a eubacteria-type cell division site-determining factor CrMinD gene to the nucleus from the chloroplast genome in Chlamydomonas reinhardtii
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Transfer of a eubacteria-type cell division site-determining factor CrMinD gene to the nucleus from the chloroplast genome in Chlamydomonas reinhardtii

机译：从莱茵衣藻中将真细菌型细胞分裂位点决定因子CrMinD基因从叶绿体基因组转移到核中

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摘要

MinD is a ubiquitous ATPase that plays a crucial role in selection of the division site in eubacteria, chloroplasts, and probably Archaea. In four green algae, Mesostigma viride, Nephroselmis olivacea, Chlorella vulgaris and Prototheca wickerhamii, MinD homologues are encoded in the plastid genome. However, in Arabidopsis, MinD is a nucleus-encoded, chloroplast-targeted protein involved in chloroplast division, which suggests that MinD has been transferred to the nucleus in higher land plants. Yet the lateral gene transfer (LGT) of MinD from plastid to nucleus during plastid evolution remains poorly understood. Here, we identified a nucleus-encoded MinD homologue from unicellular green alga Chlamydomonas reinhardtii, a basal species in the green plant lineage. Overexpression of CrMinD in wild type £. coli inhibited cell division and resulted in the filamentous cell formation, clearly demonstrated the conservation of the MinD protein during the evolution of photosynthetic eukaryotes. The transient expression of CrMinD-egfp confirmed the role of CrMinD protein in the regulation of plastid division. Searching all the published plastid genomic sequences of land plants, no MinD homologues were found, which suggests that the transfer of MinD from plastid to nucleus might have occurred before the evolution of land plants.
机译：MinD是一种普遍存在的ATP酶，在选择真细菌，叶绿体和古细菌中的分裂位点中起着至关重要的作用。在四个绿藻中，Mesostigma viride，Nephroselmis olivacea，Chlorella vulgaris和Prototheca wickerhamii中，MinD同源物编码在质体基因组中。但是，在拟南芥中，MinD是参与叶绿体分裂的核编码，叶绿体靶向蛋白，这表明MinD已被转移到高地植物的核中。然而，在质体进化过程中，MinD从质体向核的侧向基因转移（LGT）仍然知之甚少。在这里，我们从单细胞绿藻莱茵衣藻（Chlamydomonas reinhardtii）（一种绿色植物谱系中的基础物种）中鉴定了一个核编码的MinD同源物。野生型£中CrMinD的过表达。大肠杆菌抑制细胞分裂并导致丝状细胞形成，清楚地证明了MinD蛋白在光合真核生物进化过程中的保守性。 CrMinD-egfp的瞬时表达证实了CrMinD蛋白在调节质体分裂中的作用。搜索所有已发表的陆地植物质体基因组序列，未发现MinD同源物，这表明MinD从质体向核的转移可能发生在陆地植物进化之前。

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