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首页> 外文期刊>Chinese science bulletin >Transfer of a eubacteria-type cell division site-determining factor CrMinD gene to the nucleus from the chloroplast genome in Chlamydomonas reinhardtii
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Transfer of a eubacteria-type cell division site-determining factor CrMinD gene to the nucleus from the chloroplast genome in Chlamydomonas reinhardtii

机译：从莱茵衣藻的叶绿体基因组转移真细菌型细胞分裂位点决定因子CrMinD基因到细胞核。

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摘要

MinD is a ubiquitous ATPase that plays a crucial role in selection of the division site in eubacteria ehloroplasts, and probably Archaea. In four green algae, Mesostigma viride, Nephroselmis olivacea Chlorella vulgaris and Prototheca wickerhamii, MinDhomologues are encoded in the plastid genome. However, in Arabidopsis, MinD is a nucleus-encoded, chloroplast-targeted protein involved in chloroplast division, which suggests that MinD has been transferred to the nucleus in higher land plants Yet the lateral gene transfer (LGT) of MD from plastid to nucleus during plastid evolution remains poorly understood. Here, we identified a nucleus-encoded MinD homologue from unicellular green alga Chlamydomonas reinhardtii, a basal species in the green plantlineage. Overexpression of CrMinD in wild type E. coll inhibited cell division and resulted in the filamentous cell formation, clearly demonstrated the conservation of the MinD protein during the evolution of photosynthetic eukaryotes The transient expression of CrMinD-egfp confirmed the role of CrMinD protein in the regulation of plastid division. Searching all the published plastid genomic sequences of land plants, no MinD homologues were found, which suggests that the transfer of MD from plastid tonucleus might have occurred before the evolution of land plants.
机译：MinD是一种普遍存在的ATP酶，在真细菌叶绿体（可能是古细菌）的分裂位点的选择中起着至关重要的作用。在四个绿藻中，Mesostigma viride，Nephroselmis olivacea Chlorella vulgaris和Prototheca wickerhamii中的MinDhomologues被编码在质体基因组中。然而，在拟南芥中，MinD是一种参与叶绿体分裂的核编码，叶绿体靶向蛋白，这表明MinD已被转移到高地植物的核中，但M / nD的侧向基因转移（LGT）是从质体向质体进化过程中细胞核仍然知之甚少。在这里，我们从单细胞绿藻莱茵衣藻（Chlamydomonas reinhardtii）（一种绿色植物谱系中的基础物种）中鉴定了一个核编码的MinD同源物。 CrMinD在野生型大肠杆菌中的过表达抑制细胞分裂并导致丝状细胞形成，清楚地证明了光合真核生物进化过程中MinD蛋白的保守性。CrMinD-egfp的瞬时表达证实了CrMinD蛋白在调控中的作用。质体分裂。搜索所有已发表的陆地植物质体基因组序列，未发现MinD同源物，这表明M / nD从质体核转移可能是在陆地植物进化之前发生的。

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