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首页> 外文期刊>Chinese science bulletin >Studies on the overexpression of the soybean GmNHXl in Lotus corniculatus: The reduced Na~+ level is the basis of the increased salt tolerance
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Studies on the overexpression of the soybean GmNHXl in Lotus corniculatus: The reduced Na~+ level is the basis of the increased salt tolerance

机译：大豆Cormulatus中大豆GmNHX1过表达的研究：降低的Na〜+水平是提高耐盐性的基础

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摘要

The full length cDNA coding for a novel vacuolar Na~+/H~+ antiporter (GmNHXl) was cloned from soybean and determined to consist of 2591 bp with a 5'-untranslated region of 464 bp, an open reading frame (ORF) of 1641 bp, and a 3'-untrans-lated region of 486 bp. The deduced protein sequence contains 546 aa with the typical characters of the vacuolar Na~+/H~+ antiporters, and shares high similarity with that of AtNHXI, OsNHXI and AgNHXI. The soybean genome showed a single copy of the GmNHXl gene. Northern blot analysis demonstrated that the expression of the GmNHXl was tissue-specific, increased by ABA treatment, NaCI, KCI, LiCI and dehydration stress, and lower in leaves but higher in roots and hypocotyls of salt-tolerant than salt-sensitive cultivars. The GmNHXl was overex-pressed under the control of a tandem cauliflower mosaic virus (CaMV) 35S promoter in the model leguminous plant Lotus corniculatus L. and conferred salt-tolerance of the transgenic plants. Measurements of Na~+ and K~+ contents inboth roots and shoots demonstrated that the plantlets of GmNHXI-overexpressing lines had lower Na~+ and K~+ content, and higher K~+/Na~+ ratio than the controlled lines that were transformed with the empty vector, which indicates that the salt-toleranceconferred by GmNHXl is closely related with decreased accumulation of Na~+ in the transgenic plants.
机译：从大豆中克隆出编码新型液泡Na〜+ / H〜+反转运蛋白（GmNHX1）的全长cDNA，确定其全长2591 bp，其中5'非翻译区为464 bp，其开放阅读框为ORF。 1641 bp，以及3'-非翻译区486 bp。推导的蛋白质序列含有546个氨基酸，具有液泡状Na〜+ / H〜+反转运蛋白的典型特征，与AtNHXI，OsNHXI和AgNHXI具有高度相似性。大豆基因组显示出GmNHX1基因的单拷贝。 Northern印迹分析表明，GmNHX1的表达是组织特异性的，通过ABA处理，NaCl，KCI，LiCl和脱水胁迫而增加，并且在叶中较低，但在根部和下胚轴中比耐盐品种高。在串状花椰菜花叶病毒（CaMV）35S启动子的控制下，GmNHX1在豆科植物莲花角顶莲的模型中过表达，并赋予了转基因植物耐盐性。对根和芽中Na〜+和K〜+含量的测量表明，与对照相比，过量表达GmNHXI的品系幼苗的Na〜+和K〜+含量较低，且K〜+ / Na〜+比例较高。用空载体转化，表明GmNHX1赋予的耐盐性与转基因植物中Na〜+的积累减少密切相关。

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