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首页> 外文期刊>Chinese science bulletin >Comparative analysis of A, B, C and D genomes in the genus Oryza with C_0t-1 DNA of C genome
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Comparative analysis of A, B, C and D genomes in the genus Oryza with C_0t-1 DNA of C genome

机译：与C基因组的C_0t-1 DNA比较分析稻属中的A，B，C和D基因组

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摘要

Fluorescence in situ hybridization (FISH) was applied to somatic chromosomes preparations of Oryza officinalis Wall. (CC), O. sativa L, (AA)xO. offi-cinalis F_1 hybrid (AC), backcross progenies BCi (AAC and ACC), O. latifolia Desv. (CCDD), O. alta Swallen (CCDD) and O. punctata Kotschy (BBCC) with a labelled probe of C_0t-1 DNA from O. officinalis. In O. officinalis, the homologous chromosomes showed similar signal bands probed by C_0t-1 DNA and karyotype analysis was conducted based on the band patterns. Using no blocking DNA, the probe Identified the chromosomes of C genome clearly, but detected few signals on chromosomes of A genome in the F_1 hybrid and two backcross progenies of BC_1. It is obvious that the highly and moderately repetitive DNA sequences were considerably different between C and A genomes. The chromosomes of C genome were also discriminated from the chromosomes of D-and B-genome in the tetraploid species O. latifolia, O. alta and O. punctata by C_0t-1 DNA-FISH. Comparison of the fluorescence intensity on the chromosomes of B, C and D genomes in O. latifolia, O. alta, and O. punctata indicated that the differentiations between C and D genomes are less than that between C and B genomes. The relationship between C and D genomes in O. alta is closer than that of C and D genomes in O. latifolia. This would be one of the causes for the fact that both the genomes are of the same karyotype (CCDD) but belong to different species. The above results showed that the Cot-1 DNA had a high specificity of genome and species. In this paper, the origin of allotetraploid in genus Oryza is also discussed.
机译：荧光原位杂交（FISH）应用于稻米壁的体细胞染色体制备。 （CC），水稻（O. sativa L），（AA）xO。 offi-cinalis F_1杂种（AC），回交后代BCi（AAC和ACC），O。latifolia Desv。 （CCDD），O。alta Swallen（CCDD）和O.punctata Kotschy（BBCC），以及来自O. officinalis的C_0t-1 DNA标记探针。在O. officinalis中，同源染色体显示出由C_0t-1 DNA探测到的相似信号带，并根据带型进行了核型分析。该探针不使用封闭性DNA，可以清楚地鉴定C基因组的染色体，但在F_1杂种和BC_1的两个回交子代中，在A基因组的染色体上几乎检测不到信号。显然，C和A基因组之间的高度和中等重复性DNA序列差异很大。还通过C_0t-1 DNA-FISH将四倍体物种O. latifolia，O。alta和O. punctata的D-基因组和B-基因组的染色体与C基因组的染色体区分开。比较了O. latifolia，O。alta和O. punctata中B，C和D基因组染色体的荧光强度，发现C和D基因组之间的差异小于C和B基因组之间的差异。 O. alta中C和D基因组之间的关系比O. latifolia中C和D基因组之间的关系更紧密。这可能是两个基因组具有相同核型（CCDD）但属于不同物种的事实的原因之一。以上结果表明，Cot-1 DNA具有高的基因组和物种特异性。本文还讨论了稻米属中异源四倍体的起源。

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