Pep tide segment ligation: A new method for synthesis of peptide and protein

MA Yuan; ZHAO Yufen

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Pep tide segment ligation: A new method for synthesis of peptide and protein

机译：肽段连接：合成肽和蛋白质的新方法

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The protein structure-function relationships are always highlighted in the field of life science. Protein synthesis from genomic sequence data is gaining significance in the "post-genomic era" of biomedical research by providing direct access to functional proteins. The manually or automatically stepwise solid phase peptide synthesis (SPPS) allows peptide of up to 60 residues to be routinely constructed in good yield and high purity. The assembly of longer proteins via the gene engineering technology (e.g. recombinant DNA-based molecular biology or site-directed mutagenesis) and convergent peptide synthesis are necessary. Although the current biosynthetic method allows unnatural amino acids to be incorporated into proteins or peptides, only α-peptide in the protein backbone can be obtained. A lot of problems associated with the classic convergent peptide synthesis approach, such as the poor solubility, inadequate purification techniques, and limited characterization methods with the fully protected segment. However, totally chemical synthetic method can easily obtain β- or γ-peptide and even branch peptide.

机译：蛋白质结构与功能的关系在生命科学领域中总是被强调。通过直接访问功能性蛋白质，从基因组序列数据合成蛋白质在生物医学研究的“后基因组时代”中正变得越来越重要。手动或自动逐步固相肽合成（SPPS）可以常规方式以高收率和高纯度构建多达60个残基的肽。必须通过基因工程技术（例如，基于重组DNA的分子生物学或定点诱变）和更长的肽合成来组装更长的蛋白质。尽管当前的生物合成方法允许将非天然氨基酸掺入蛋白质或肽中，但是只能获得蛋白质骨架中的α-肽。与经典的融合肽合成方法有关的许多问题，例如溶解性差，纯化技术不足以及完全保护的片段的表征方法有限。然而，完全化学合成方法可以容易地获得β-或γ-肽，甚至支链肽。

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《Chinese science bulletin》 |2003年第1期|p.1-5|共5页
作者
MA Yuan; ZHAO Yufen;
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Bioorganic Phosphorus Chemistry Laboratory of Educational Ministry, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China;

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正文语种 eng
中图分类自然科学现状及发展;
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6. A straightforward methodology to overcome solubility challenges for N-terminal cysteinyl peptide segments used in native chemical ligation [O] . Skander A. Abboud, El hadji Cisse, Michel Doudeau, 2021

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Pep tide segment ligation: A new method for synthesis of peptide and protein

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