Imaging surface structure of purple membrane partly dissolved by CHAPS with atomic force microscope

Li Hui; Chen Deliang; Hu Kunsheng; Han Baoshan

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Imaging surface structure of purple membrane partly dissolved by CHAPS with atomic force microscope

机译：CHAPS原子力显微镜对部分溶解的紫色膜的成像表面结构的成像

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Atomic force microscope was employed to study the surface structure of purple membrane (PM) using contact mode in liquid. The surface structures of native PM and PM partly dissolved by detergent CHAPS were compared. Results show that 6 mmol/L CHAPS could induce some cracks and holes in PM. While Bateriorhodopsin (BR) was still presented as trimers in the two-dimensional hexagonal structure. We assume that it was caused by the interaction between CHAPS micellar and PM. Both absorption spectra and flash photolysis kinetic spectra indicated that BR's biochemical functions have been changed.

机译：利用原子力显微镜，采用液体接触模式研究了紫色膜的表面结构。比较了天然PM和被洗涤剂CHAPS部分溶解的PM的表面结构。结果表明，6 mmol / L CHAPS会引起PM的一些裂纹和孔洞。尽管视紫红质细菌视紫红质（BR）在二维六边形结构中仍作为三聚体存在。我们假设这是由CHAPS胶束和PM之间的相互作用引起的。吸收光谱和快速光解动力学光谱均表明BR的生化功能已改变。

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来源
《Chinese science bulletin》 |2003年第6期|p. 573-576|共4页
作者
Li Hui; Chen Deliang; Hu Kunsheng; Han Baoshan;
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作者单位

State Key Laboratory of Magnetism, Institute of Physics, Center of Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China;

Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;

Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;

State Key Laboratory of Magnetism, Institute of Physics, Center of Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China;

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正文语种 eng
中图分类自然科学现状及发展;
关键词
purple membrane; bacteriorhodopsin; detergent; CHAPS; atomic force microscope;

机译：紫色膜;细菌视紫红质;洗涤剂;CHAPS;原子力显微镜;

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专利

1. Imaging surface structure of purple membrane partly dissolved by CHAPS with atomic force microscope [J] . Li Hui, Chen Deliang, Hu Kunsheng, Chinese science bulletin . 2003,第6期

机译：CHAPS原子力显微镜对部分溶解的紫色膜的成像表面结构的成像
2. Investigation of structural change of purple membrane in storage by transmission electron microscope and atomic force microscope [J] . Li Xiaodong, Li Zongli, Yan Jiusheng Chinese science bulletin . 2001,第20期

机译：透射电子显微镜和原子力显微镜研究贮藏紫色膜的结构变化
3. ATOMIC FORCE MICROSCOPE STUDIES OF MEMBRANES - SURFACE PORE STRUCTURES OF DIAFLO ULTRAFILTRATION MEMBRANES [J] . Bowen WR., Lovitt RW., Williams PM., Journal of Colloid and Interface Science . 1996,第2期

机译：膜的原子力显微镜研究-非球形超滤膜的表面孔结构。
4. Fast Simulation of Atomic-Force-Microscope Imaging of Atomic and Polygonal Surfaces Using Graphics Hardware [C] . Gokul Varadhan, Warren Robinett, Dorothy Erie, Conference on Visualization and Data Analysis 2002, Jan 21-22, 2002, San Jose, USA . 2002

机译：使用图形硬件对原子和多边形表面进行原子力显微镜成像的快速仿真
5. Quantitative imaging of subsurface structures and mechanical properties at nanoscale using atomic force microscope. [D] . Parlak, Zehra. 2010

机译：使用原子力显微镜在纳米尺度下对地下结构和力学性能进行定量成像。
6. Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis [O] . Stefan W. Schneider, Kumudesh C. Sritharan, John P. Geibel, 1997

机译：原子力成像的活腺泡细胞的表面动力学显微镜：鉴定所涉及的质膜结构胞吐
7. Fast Simulation of Atomic-Force-Microscope Imaging of Atomic and Polygonal Surfaces Using Graphics Hardware [O] . Gokul Varadhan, Warren Robinett, Dorothy Erie, 2002

机译：使用图形硬件对原子和多边形表面的原子力显微镜成像进行快速仿真
8. Imaging Purple Membranes Dry and in Water with the Atomic Force Microscope [R] . Butt, H. J., Prater, C. B., Hansma, P. K. 1991

机译：用原子力显微镜成像干燥和水中的紫膜

Imaging surface structure of purple membrane partly dissolved by CHAPS with atomic force microscope

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