Na~+-permeable channels of human sperm membrane re - assembled into giant liposome

Zhang Guangping; Bai Junping; Shi Yuliang

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Na~+-permeable channels of human sperm membrane re - assembled into giant liposome

机译：精子的Na〜+渗透通道重新组装成巨大的脂质体

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Previous data showed that a Na-transmem - brane flux was accompanied with acrosome reaction of sperm. However, the electrophysiological recording and characterization of Na current in human sperm membrane have not been yet reported. In the present investigation, membrane proteins extracted from human sperms were reassembled into liposome bilayer, and then the liposomes were fused y dehydration - rebydration into giant liposomes with the diameter of more than 10 μm.

机译：先前的数据表明，Na-跨膜-麸皮通量伴随着精子的顶体反应。但是，尚未报道人类精子膜中Na电流的电生理记录和表征。在本研究中，将从人精子中提取的膜蛋白重新组装成脂质体双层，然后通过脱水融合脂质体-将其重配成直径大于10μm的巨型脂质体。

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《Chinese science bulletin》 |2001年第13期|p.1085-1088|共4页
作者
Zhang Guangping; Bai Junping; Shi Yuliang;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
patch clamp; giant liposome; single channel;

机译：膜片夹;巨脂质体单通道;

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1. A patch‐clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes [J] . Berrier Catherine, Coulombe Alain, Ghazi Alexandre, FEBS Letters . 1989,第1期

机译：膜片钳研究融合到巨大脂质体中的内，外膜离子通道和大肠杆菌接触区
2. Membrane permeability and stability in buffer and in human serum of fluorinated di-O-alkylglycerophosphocholine-based liposomes [J] . Véronique Ravily, Catherine Santaella, Pierre Vierling Biochimica et biophysica acta. Biomembranes . 1996,第1期

机译：氟化的基于二邻烷基烷基甘油磷酸胆碱的脂质体在缓冲液和人血清中的膜通透性和稳定性
3. Ion-channels in human sperm membrane and contraceptive mechanisms of male antifertility compounds derived from Chinese traditional medicine [J] . SHI Yu-Liang, BAI Jun-Ping, WANG Wen-Ping Acta Pharmacologica Sinica . 2003,第1期

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4. Display of Recombinant Membrane Receptors on Giant Liposomes: Attempt to Construct a Cell Model with Integrated Membrane Protein Systems [C] . Kanta Tsumoto, Koki Kamiya, Tetsuro Yoshimura International Symposium on Micro-NanoMechatronics and Human Science . 2007

机译：巨型脂质体上的重组膜受体显示：试图用综合膜蛋白系统构建细胞模型
5. Characterization of membrane organization and dynamics in giant unilamellar vesicles assembled from rat kidney membrane extracts and fragments. [D] . Cheng, Melanie Anne B. 2005

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6. Highly permeable artificial water channels that can self-assemble into two-dimensional arrays [O] . Yue-xiao Shen, Wen Si, Mustafa Erbakan, 2015

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7. A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes Pressure-activated channels are localized in the inner membrane [O] . Berrier Catherine, Coulombe Alain, Houssin Christine, 1989

机译：膜片钳研究融合到巨大脂质体中的内，外膜离子通道和大肠杆菌接触区的压力激活通道位于内膜中

Na~+-permeable channels of human sperm membrane re - assembled into giant liposome

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