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Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)

机译:水稻(Oryza sativa)核糖体蛋白S4编码cDNA的克隆与表达

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摘要

A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80S ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the Expression level varies and it cannot be induced by mechanical wounding in leaves.
机译:已经从由约1.0mm长的花药制成的cDNA文库中分离出cDNA克隆pS4。 DNA序列分析和数据库搜索表明该cDNA编码的蛋白质与真核80S核糖体蛋白亚基4(S4)高度同源。 Northern杂交表明该基因在所有分析的组织中表达,尽管表达水平变化并且不能被叶片的机械损伤诱导。

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