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Primary structure of helix C to helix G of a new retinal protein in H. Sp. Xz515

机译:H. Sp。中一种新的视网膜蛋白螺旋C至螺旋G的一级结构。 Xz515

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摘要

The gene fragment encoding the retinal protein from helix C to helix G in a new strain of halobacteria, H. Sp.xz515 has been amplified by PCR method. The nucleotide sequence of this fragment has been determined. The deduced amino acid sequence has been compared with halobium br and other two br-like proteins, ar -1 and ar-2. Results show that those amino acid residues in br, essential for proton pumping and binding to retinal, are conserved. The residue M145 in br may be important for isomerization reaction of retinal.
机译:通过PCR方法扩增了一种新的盐杆菌菌株H. Sp.xz515中编码从螺旋C到螺旋G的视网膜蛋白的基因片段。该片段的核苷酸序列已经确定。推导的氨基酸序列已与ha br和其他两个br类蛋白ar -1和ar-2进行了比较。结果表明,br中质子泵和与视网膜结合必不可少的br氨基酸残基是保守的。 br中的M145残基可能对视网膜的异构化反应很重要。

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