• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chinese science bulletin >Transient transformation of pollen protoplasts via electroporation and temporal expression of Zm13-260-GUS-NOS chimeric gene in Brassica campestris var. purpurea
【24h】

Transient transformation of pollen protoplasts via electroporation and temporal expression of Zm13-260-GUS-NOS chimeric gene in Brassica campestris var. purpurea

机译：通过电穿孔和Zm13-260-GUS-NOS嵌合基因在甘蓝型油菜中的花粉原生质体的瞬时转化。紫癜

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using pollen as a vector of foreign genes for plant transformation is an active research area. An important link in this transformation system is the delivery of foreign genes into pollen. However, DNA transfer into pollen is difficult because of theexistence of a thick pollen wall, whereas pollen protoplast deprived of pollen wall should be advantageous in this respect. The isolation and culture of pollen protoplasts have made progress in recent years, so that the use of pollen protoplasts as transformation target becomes possible. For this purpose, we used the GUS gene controlled by pollen-specific promoter Zm13-260 as reporter gene for transient expression assay after electroporation and compared the transformation effects in pollen protoplastsand pollen grains. We also investigated the temporal expression of the GUS gene in electroporated pollen protoplasts at various developmental stages.
机译：使用花粉作为外源基因的载体进行植物转化是一个活跃的研究领域。该转化系统中的重要环节是将外源基因传递到花粉中。然而，由于存在厚的花粉壁，DNA难以转移到花粉中，而从花粉壁剥夺的花粉原生质体在这方面应是有利的。近年来，花粉原生质体的分离和培养取得了进展，因此将花粉原生质体用作转化目标成为可能。为此，我们使用由花粉特异性启动子Zm13-260控制的GUS基因作为报告基因进行电穿孔后的瞬时表达测定，并比较了在花粉原生质体和花粉粒中的转化效果。我们还研究了在不同发育阶段电穿孔花粉原生质体中GUS基因的时间表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号