Molecular cloning, chromosomal mapping and expression analysis of disease resistance gene homologues in rice (oryza sativa L.)

Li Ziyin; Chen Shouyi

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Molecular cloning, chromosomal mapping and expression analysis of disease resistance gene homologues in rice (oryza sativa L.)

机译：水稻抗病基因同源物的分子克隆，染色体作图及表达分析

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Resistance-like sequences have been amplified from first strand cDNA and genomic DMA of rice by PCR using oligonucleotide primers designed from sequence motifs conserved Between resistance genes of tobacco and Arbidopsis thaliana. 3 PCR clones, designated Osr1, Osr2 and Osr3 which were 98/100 identical in nucleotide sequence level, have been found To be significantly homologous to known plant resistance genes and all contained the conserved Motifs of NBS-LRR type resistance genes, such as P-loop, kinse2a, kinase3a and transmem- Brane domain.

机译：使用从烟草和拟南芥的抗性基因之间保守的序列基序设计的寡核苷酸引物，通过PCR从水稻的第一链cDNA和基因组DMA扩增了抗性样序列。已发现3个PCR克隆，命名为Osr1，Osr2和Osr3，它们在核苷酸序列水平上具有98/100相同，与已知的植物抗性基因显着同源，并且均包含NBS-LRR型抗性基因的保守基序，例如P -环，kinse2a，激酶3a和跨膜Brane结构域。

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《Chinese science bulletin》 |1999年第13期|p.1202-1207|共6页
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Li Ziyin; Chen Shouyi;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
rice; resistance gene homologue; rice blast;

机译：白饭;抗性基因同源物稻瘟病;

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1. Molecular progress on the mapping and cloning of functional genes for blast disease in rice (Oryza sativa L.): current status and future considerations (vol 36, pg 1, 2014) [J] . Ashkani S., Rafii M. Y., Shabanimofrad M., Critical Reviews in Biotechnology . 2016,第1a6期

机译：稻瘟病功能基因的定位和克隆的分子研究进展：现状和未来考虑（第36卷，第1页，2014年）
2. Molecular progress on the mapping and cloning of functional genes for blast disease in rice (Oryza sativa L.): current status and future considerations [J] . Ashkani S., Rafii M. Y., Shabanimofrad M., Critical Reviews in Biotechnology . 2016,第1a6期

机译：水稻（Oryza sativa L.）稻瘟病功能基因的定位和克隆的分子进展：现状和未来考虑
3. Molecular progress on the mapping and cloning of functional genes for blast disease in rice (Oryza sativa L.): current status and future considerations (vol 36, pg 1, 2014) [J] . Ashkani S., Rafii M. Y., Shabanimofrad M., Critical Reviews in Biotechnology . 2016,第1a6期

机译：水稻（Oryza Sativa L.）爆炸疾病函数基因的测绘和克隆的分子进展：现状和未来考虑（Vol 36，PG 1，2014）
4. THE SEMI-DWARF GENE, sd-1, OF RICE: (ORYZA SATIVA L.) CONSTRUCTION OF A RICE YAC LIBRARY FOR MAP BASED CLONING [C] . M.Y. EUN, J.H. HAHN, Y.G. CHO, Induced mutations and molecular techniques for crop improvement . 1995

机译：水稻的半矮基因sd-1：（ORYZA SATIVA L.）基于地图克隆的水稻YAC文库的构建
5. Evaluation and molecular mapping of rice tungro resistance genes of rice (Oryza sativa L.). [D] . Sebastian, Leocadio Sotor. 1994

机译：水稻抗稻瘟病基因的评估和分子作图。
6. Genome-Wide Identification Classification and Expression Analysis of Autophagy-Associated Gene Homologues in Rice (Oryza sativa L.) [O] . Kuaifei Xia, Tao Liu, Jie Ouyang, 2011

机译：水稻（Oryza sativa L.）自噬相关基因同源基因的全基因组鉴定分类和表达分析
7. Genome-Wide Identification, Classification, and Expression Analysis of Autophagy-Associated Gene Homologues in Rice (Oryza sativa L.) [O] . Xia, Kuaifei, Liu, Tao, Ouyang, Jie, 2011

机译：水稻（Oryza sativa L.）自噬相关基因同源基因的全基因组鉴定，分类和表达分析

Molecular cloning, chromosomal mapping and expression analysis of disease resistance gene homologues in rice (oryza sativa L.)

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