Cloning and sequencing of phbA gene of poly-β-hydroxybuty rate synthesis and its expression analysis

Ye Liang; Li Cong; Song Yanru

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Cloning and sequencing of phbA gene of poly-β-hydroxybuty rate synthesis and its expression analysis

机译：-β-羟基丁酸酯合成的phbA基因的克隆，序列分析及表达分析

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The gene of the first key enzyme of poly-β-hydroxybutyrate synthesis, 3-ketothio- lase, has been amplified and cloned from chromosomal DNA of Alcaligenes eutrophus H16 by PCR. DNA sequencing results show that phbA cloned in pBluescriptSK~+ has an identical se- Quence with that reported previously except for one baser pair. The plant constitutive expression Vector has been constructed and tobacco has been transformed in order to examine the phbA Gene function and the efficiency of ctp gene product.

机译：已经通过PCR从拟南芥H16的染色体DNA上扩增并克隆了聚β-羟基丁酸酯合成的第一个关键酶3-酮硫醇酶的基因。 DNA测序结果表明，克隆在pBluescriptSK〜+中的phbA具有与先前报道的相同的序列，只是一对碱基对除外。为了检查phbA基因的功能和ctp基因产物的效率，已经构建了植物组成型表达载体，并对烟草进行了转化。

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《Chinese science bulletin》 |1999年第15期|p.1387-1391|共5页
作者
Ye Liang; Li Cong; Song Yanru;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
phbA gene; poly-β-hydroxybutyrate; 3-ketothiolase;

机译：phbA基因;聚-β-羟基丁酸酯;3-酮硫解酶;

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1. Cloning, Sequencing and Characterization of 3-Hydroxybuty- rate Dehydrogenase Encoding Gene (bdh A) in Bradyrhizobium japonicum USDA110 Strain [J] . DAI Mei-xue, WU Bo, BAI Xue-liang, Agricultural sciences in China . 2002,第9期

机译：日本根瘤菌根瘤菌USDA110菌株3-羟丁酸速率脱氢酶编码基因（bdh A）的克隆，序列分析与鉴定
2. Cloning, Sequencing and Characterization of 3-Hydroxybuty- rate Dehydrogenase Encoding Gene (bdh A) in Bradyrhizobium japonicum USDA110 Strain [J] . DAI Mei-xue, WU Bo, BAI Xue-liang, 农业科学学报（英文版） . 2002,第009期

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3. Molecular cloning, sequencing and expression in Escherichia coli of thepoly(3-hydroxyalkanoate) synthesis genes from Alcaligenes latus DSM1124 [J] . Genser KF., Renner G., Schwab H. Journal of Biotechnology . 1998,第2a3期

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Cloning and sequencing of phbA gene of poly-β-hydroxybuty rate synthesis and its expression analysis

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